Gibberellin and abscisic acid transporters facilitate endodermal suberin formation in Arabidopsis

Sharon, Eilon; Binenbaum, Jenia; Wulff, Nikolai; Camut, Lucie; Kiradjiev, Kristian; Tal, Iris; Vasuki, Himabindu; Anfang, Moran; Zhang, Yuqin; Sakvarelidze-Achard, Lali; Davière, Jean-Michel; Ripper, Dagmar; Carrera, Esther; Manasherova, Ekaterina; Lazary, Shani; Novák, Vlastimil; Crocoll, Christoph; Weinstein, R.; Cohen, Hagai; Ragni, Laura; Aharoni, Asaph; Band, Leah R.; Achard, Patrick; Nour-Eldin, Hussam Hassan

doi:10.21203/rs.3.rs-1670556/v1

Cited by 6 publications

(7 citation statements)

References 54 publications

(85 reference statements)

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“…However, since the suberization pattern as well as PHO1:H3 expression in myb68 roots was least affected in PPAE and NPAE cells, additional MYB68-independent mechanisms must exists which positively affects suberization decision in the PPAE. This is supported by a recent study which, via a modeling approach, proposed a role for GA in PPAE suberization (Binenbaum, et al, 2023). Moreover, our analyses expands on this model by proposing that the hormonal sensitivity of the XPAE cells is not solely determined by AHP6, but likely also other unknown cytokinin-responseive factors present in the XPAE (Figure 6A).…”

Section: Discussionsupporting

confidence: 86%

MYB68 regulates suberin patterning and radially distinct endodermal differentiation

Kraska,

Nakano,

Molina

et al. 2024

Preprint

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Roots are composed of concentric tissue layers that enable selective uptake of nutrients, attributed to the vascular-embracing endodermis. In mature root regions, endodermal cells coat their cell surface in a hydrophobic polymer termed suberin, which blocks solute transport across the plasma membrane. Intriguingly, specific cells adjacent to the water-conducting xylem remain unsuberized. These are termed passage cells based on the assumption that they create a passage for nutrient influx into vasculature in the otherwise sealed-off root regions. The identity and function of passage cells remain unknown, but their presence proposes the existence of individual identities within the endodermis. In this study, we probe this by performing an in-depth investigation of passage cells and endodermal suberization in the model plant Arabidopsis thaliana. Our work identifies the transcription factor MYB68 as a key regulator of suberization and endodermal lineage-specification related to passage cell formation. Through transcriptional profiling of myb68 mutants, we unravel genes with passage cell-associated expression that suggest a function in cation homeostasis. Collectively, our findings create a framework for a deeper understanding of radial cell identities within the endodermis and highlight that dynamic spatiotemporal patterning is an important physiological feature of root ground tissues.

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Section: Discussionsupporting

confidence: 86%

MYB68 regulates suberin patterning and radially distinct endodermal differentiation

Kraska,

Nakano,

Molina

et al. 2024

Preprint

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“…The degradation of DELLA by GA in darkgrown roots releases these TFs and probably triggers transcriptional activation of numerous pathways independently of light. These may include the regulation of root architecture (Castro-Camba et al, 2022), secondary xylem development, lignification (Wang et al, 2017) and endodermal suberin formation (Binenbaum et al, 2023), but not cell elongation. Several DELLA-interacting TFs are involved in GA regulation of cell elongation (de Lucas et al, 2008;Feng et al, 2008;Gallego-Bartolome et al, 2012;Chaiwanon & Wang, 2015).…”

Section: Discussionmentioning

confidence: 99%

Red light perception by the root is essential for gibberellin-induced primary-root elongation in tomato

Ramon,

Adiri,

Cheriker

et al. 2023

Preprint

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SummaryThe promoting effect of gibberellin (GA) on primary-root elongation was demonstrated in several species, including Arabidopsis (Arabidopsis thaliana). However, in some other species its effect is less clear.We have used the tomato (Solanum lycopersicum) GA-deficient mutantsgib-1andga 20-oxidase(ga20ox1) to study the role of GA in primary-root elongation and examined the involvement of light in this response.Application of GA togib-1following germination, promoted primary-root elongation, but later, when roots were deeper in the dark, GA had no effect. RNA-seq analysis revealed a typical GA transcriptional response in dark-grown roots, but the output toward cell-expansion was blocked. When dark-grown roots were illuminated deep in the ground, the hormone promoted their elongation. We demonstrated that direct red light to the root is essential for GA-induced elongation. A similar light dependent GA-elongation response was found in wheat (Triticum aestivum) roots, but not in Arabidopsis.We suggest that in tomato GA promotes root elongation following germination when roots are exposed to low light intensity underground, and this contributes to seedling establishment. As root grow deeper into the soil, the insensitivity to GA may be important for continues root growth under fluctuating water availability, as water deficiency suppresses GA accumulation.

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“…This is closely related to the aforementioned involvement of ABA in suberization. In addition, it has been reported that gibberellin and ABA accumulate in the root endodermis via NPF2.14 acting as a subcellular transporter, and the accumulated hormones regulate suberization [ 107 ]. However, the regulatory network through in which auxin and GA control suberization remains largely unknown.…”

Section: Suberin Biosynthesis In Plant Roots and Drought-derived Modi...mentioning

confidence: 99%

Hormonal Crosstalk and Root Suberization for Drought Stress Tolerance in Plants

2022

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Higher plants in terrestrial environments face to numerous unpredictable environmental challenges, which lead to a significant impact on plant growth and development. In particular, the climate change caused by global warming is causing drought stress and rapid desertification in agricultural fields. Many scientific advances have been achieved to solve these problems for agricultural and plant ecosystems. In this review, we handled recent advances in our understanding of the physiological changes and strategies for plants undergoing drought stress. The activation of ABA synthesis and signaling pathways by drought stress regulates root development via the formation of complicated signaling networks with auxin, cytokinin, and ethylene signaling. An abundance of intrinsic soluble sugar, especially trehalose-6-phosphate, promotes the SnRK-mediated stress-resistance mechanism. Suberin deposition in the root endodermis is a physical barrier that regulates the influx/efflux of water and nutrients through complex hormonal and metabolic networks, and suberization is essential for drought-stressed plants to survive. It is highly anticipated that this work will contribute to the reproduction and productivity improvements of drought-resistant crops in the future.

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Gibberellin and abscisic acid transporters facilitate endodermal suberin formation in Arabidopsis

Cited by 6 publications

References 54 publications

MYB68 regulates suberin patterning and radially distinct endodermal differentiation

MYB68 regulates suberin patterning and radially distinct endodermal differentiation

Red light perception by the root is essential for gibberellin-induced primary-root elongation in tomato

Hormonal Crosstalk and Root Suberization for Drought Stress Tolerance in Plants

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