Gibberellin 20-oxidase promotes initiation and elongation of cotton fibers by regulating gibberellin synthesis

Xiao, Yazhong; Li, De-Mou; Yin, Meng; Li, Xianbi; Mi, Zhang; Wang, Yanjun; Dong, Jing; Jun, Zhao; Luo, Ming; Luo, Xiaoying; Hou, Lei; Hu, Lin; Pei, Yan

doi:10.1016/j.jplph.2010.01.003

Cited by 126 publications

(78 citation statements)

References 17 publications

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“…Regulation of fiber development by phytohormones has been evaluated by modification of phytohormone biosynthesis or responsiveness in transgenic cotton plants. Luo et al (2007) showed that increased expression of GhDET2, a steroid 5d-reductase critical for brassinosteroid biosynthesis, increased fiber initiation and elongation and similar results were recently reported for cotton plants with increased expression of GA 20 oxidase (Xiao et al 2010). …”

Section: Discussionsupporting

confidence: 79%

Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation

Lee

Burns

Light

et al. 2010

Planta

117

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Plant cell wall extensibility is mediated, in part, by xyloglucan endotransglycosylases/hydrolases (XTH) that are able to cleave and reattach xyloglucan polymers that make up the hemicelluloses matrix of type I cell walls. In Arabidopsis and other plants, XTHs are encoded by relatively large gene families that are regulated in specific spatial and temporal patterns. In silico screening of a cotton expressed sequence tag (EST) database identified 23 sequences with close sequence similarity to Arabidopsis XTH coding sequences. Analysis of full-length cotton cDNAs derived from these ESTs allow for the identification of three distinct GhXTH cDNAs (denoted GhXTH1, GhXTH2 and GhXTH3) based primarily on their 3' untranslated sequences. The three GhXTH genes were expressed differently with GhXTH1 predominantly expressed in elongating cotton fibers. The function of GhXTH1 in mediating cotton fiber elongation was analyzed in transgenic cotton plants that express a transgene consisting of the GhXTH1 coding sequence under transcriptional control of the CaMV 35S promoter. Plants that over-expressed GhXTH1 had increased XTH activity and produced mature cotton fibers that were between 15 and 20% longer than wild-type cotton plants under both greenhouse and field growth conditions. Segregation analysis showed that the 35S::GhXTH1 transgene acts as a dominant fiber length allele in transgenic cotton. These results confirm that GhXTH1 is the predominant XTH in elongating fibers and its expression limits cotton fiber elongation.

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Section: Discussionsupporting

confidence: 79%

Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation

Lee

Burns

Light

et al. 2010

Planta

117

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“…These results demonstrate that GbWRKY1 might be an important negative regulator in the JA signaling pathway. Although the transcript of GbWRKY1 barely responded to GA treatment (data not shown), the overexpression cotton lines exhibited morphological phenotypes similar to those of GA-overdose cotton plants under normal growth conditions as described by Xiao et al (2010), including petiole elongation and pale green leaves (Fig. 3, A, C, and E).…”

Section: Gbwrky1 Negatively Regulates the Ja Signaling Pathwaymentioning

confidence: 62%

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

Luo

et al. 2014

Plant Physiology

133

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Plants have evolved an elaborate signaling network to ensure an appropriate level of immune response to meet the differing demands of developmental processes. Previous research has demonstrated that DELLA proteins physically interact with JASMONATE ZIM-DOMAIN1 (JAZ1) and dynamically regulate the interaction of the gibberellin (GA) and jasmonate (JA) signaling pathways. However, whether and how the JAZ1-DELLA regulatory node is regulated at the transcriptional level in plants under normal growth conditions or during pathogen infection is not known. Here, we demonstrate multiple functions of cotton (Gossypium barbadense) GbWRKY1 in the plant defense response and during development. Although GbWRKY1 expression is induced rapidly by methyl jasmonate and infection by Verticillium dahliae, our results show that GbWRKY1 is a negative regulator of the JA-mediated defense response and plant resistance to the pathogens Botrytis cinerea and V. dahliae. Under normal growth conditions, GbWRKY1-overexpressing lines displayed GA-associated phenotypes, including organ elongation and early flowering, coupled with the down-regulation of the putative targets of DELLA. We show that the GA-related phenotypes of GbWRKY1-overexpressing plants depend on the constitutive expression of Gossypium hirsutum GhJAZ1. We also show that GhJAZ1 can be transactivated by GbWRKY1 through TGAC core sequences, and the adjacent sequences of this binding site are essential for binding specificity and affinity to GbWRKY1, as revealed by dual-luciferase reporter assays and electrophoretic mobility shift assays. In summary, our data suggest that GbWRKY1 is a critical regulator mediating the plant defense-to-development transition during V. dahliae infection by activating JAZ1 expression.

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“…For example, targeted expression of the indole-3-acetic acid biosynthetic gene iaaM increased the number of lint fibers and fiber fineness (Zhang et al, 2011). Transgenic cotton, overexpressing gibberellin 20-oxidase, produced more fiber initials per ovule and longer fibers (Xiao et al, 2010). Another upregulated gene in fiber-bearing ovules of KTN-silenced plants was a MPK4 ortholog (TC182568).…”

Section: Discussionmentioning

confidence: 99%

Dissecting Functions of KATANIN and WRINKLED1 in Cotton Fiber Development by Virus-Induced Gene Silencing

Jian²,

Geng³

et al. 2012

Plant Physiology

101

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Most of the world's natural fiber comes from cotton (Gossypium spp.), which is an important crop worldwide. Characterizing genes that regulate cotton yield and fiber quality is expected to benefit the sustainable production of natural fiber. Although a huge number of expressed sequence tag sequences are now available in the public database, large-scale gene function analysis has been hampered by the low-efficiency process of generating transgenic cotton plants. Tobacco rattle virus (TRV) has recently been reported to trigger virus-induced gene silencing (VIGS) in cotton leaves. Here, we extended the utility of this method by showing that TRV-VIGS can operate in reproductive organs as well. We used this method to investigate the function of KATANIN and WRINKLED1 in cotton plant development. Cotton plants with suppressed KATANIN expression produced shorter fibers and elevated weight ratio of seed oil to endosperm. By contrast, silencing of WRINKLED1 expression resulted in increased fiber length but reduced oil seed content, suggesting the possibility to increase fiber length by repartitioning carbon flow. Our results provide evidence that the TRV-VIGS system can be used for rapid functional analysis of genes involved in cotton fiber development.

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Gibberellin 20-oxidase promotes initiation and elongation of cotton fibers by regulating gibberellin synthesis

Cited by 126 publications

References 17 publications

Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation

Xyloglucan endotransglycosylase/hydrolase genes in cotton and their role in fiber elongation

Cotton WRKY1 Mediates the Plant Defense-to-Development Transition during Infection of Cotton byVerticillium dahliaeby ActivatingJASMONATE ZIM-DOMAIN1Expression

Dissecting Functions of KATANIN and WRINKLED1 in Cotton Fiber Development by Virus-Induced Gene Silencing

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