Gibberellic acid, a metabolic product of the fungus Gibberella fujikuroi: Some observations on its production and isolation

Abstract

Some strains of Gibberella fujikuroi (Saw.) Wr. (conidial state Fusarium moniliforme (Sheld.) emend. Snyder & Hansen) produce metabolic products with plant‐growth‐promoting properties. The capacity to produce such substances is common in rice‐infecting strains of G. fujikuroi; isolates from other hosts only infrequently produce such metabolites. From a selected rice‐infecting strain a plant‐growth‐promoting substance has been isolated in pure form. This substance, gibberellic acid, is distinct in chemical properties from the gibberellins described by Japanese investigators. In surface culture on Raulin‐Thom medium (2.5% sucrose) yields of 40 mg./1. are obtained in 14 days at 25°. In submerged aerated culture yields approaching 200 mg./1. are obtained on Raulin‐Thom medium (4% sucrose) in 18 days at 25°. Under such conditions, although gibberellic acid begins to appear in the medium during the latter part of the phase of active growth, at least 75%, and probably more, of the total final yield is produced after maximum dry weight has been reached and rapid uptake of sugar and ammonia‐nitrogen has ceased.

Characterization of Rhizobacteria for Multiple Plant Growth Promoting Traits from Mung Bean Rhizosphere

“…Characterization of rhizobacterial isolates for IAA and GA production was done as per the methods given by Gordon and Weber (1951) and Borrow et al, (1955) respectively.…”

Potency of Bacillus spp from Potato Rhizosphere as Active Ingredients for Biostimulant Formulation

“…The production of gibberellic acid was tested according to standard methods (Borrow at al, 1995). The absorbent was measured at 254 nm on the UV-VIS spectrophotometer.…”

Functional Attributes of Psychrotolerant Rhizobacteria from Wheat (Triticum aestivum L.) Rhizoshpere

“…Characterization of isolates for the production of IAA and gibberellic acid was determined as per the method given by Gordon and Weber (1951) and Borrow et al, (1955) respectively.…”