Giardia intestinalis: Electrophoretic evidence fora species complex

Andrews, Ross H.; Adams, Mark; Boreham, P. F. L.; Mayrhofer, Graham; Meloni, Bruno P.

doi:10.1016/0020-7519(89)90006-4

Cited by 137 publications

(119 citation statements)

References 26 publications

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“…Our TPI gene sequence showed a 99.8% similarity to G. duodenalis assemblage A, group I, AD-1 strain (AF069556, Monis et al, 1999), which was originally isolated from a human duodenal aspirate and characterized by Andrews et al (1989). The ␤-giardin gene sequence showed 98.1% similarity to assemblage A (AY072724), reported by Cacciò et al (2002).…”

mentioning

confidence: 59%

Identification of Assemblage A Giardia in White-Tailed Deer

Trout¹,

Santı́n²,

Fayer³

2003

Journal of Parasitology

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confidence: 59%

Identification of Assemblage A Giardia in White-Tailed Deer

Trout¹,

Santı́n²,

Fayer³

2003

Journal of Parasitology

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“…Therefore, whilst cyst morphology may be able to distinguish between populations of Giardia in two hosts of the same species, it is inadvisable to use differences in size alone as a means of species distinction within the genus Giardia (Filice, 1952). Indeed it is the lack of reliably consistent differences between the G. duodenalis assemblages A and B that is retarding their recognition as distinct species (Thompson et al, 2000b), even though it has been demonstrated that the genetic distance separating them exceeds that used to delineate other species of protozoa ( Andrews et al, 1989, Mayrhofer et al, 1995and Monis et al, 1996. This suggests that genetic and biological data is more reliable than morphology when delineating species of Giardia.…”

Section: Discussionmentioning

confidence: 99%

Cyst morphology and sequence analysis of the small subunit rDNA and ef1α identifies a novel Giardia genotype in a quenda (Isoodon obesulus) from Western Australia

Adams

Monis

Elliot

et al. 2004

Infection, Genetics and Evolution

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Sequence analysis of the small subunit ribosomal DNA (SSU-rDNA) and elongation factor 1 alpha (ef1α) was performed on Giardia cysts isolated from faeces collected from a quenda (Isoodon obesulus) in the southwest of Western Australia. The SSU-rDNA and ef1α were sequenced in their entirety and correspondingly aligned with the published sequence information of other known species and genotypes of Giardia. Phylogenetic analysis of the SSU-rDNA and ef1α sequences identified the quenda isolate as a novel genotype of Giardia not previously reported. We believe that this quenda Giardia isolate constitutes a distinct species, which may be endemic within the Australian native fauna.

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“…Sequences were then sorted and grouped into their assemblages and subassemblages (where possible) according to the positions of their single nucleotide polymorphisms (SNPs) relative to previously characterized reference isolates. The isolates used as subassemblage reference isolates included AI -WB, Portland 1 and/or Ad-1, AII -JH, AB, KC8, Ad-2, Bris-136 and/or Ad-113, BIII -BAH-12 and BIV -Ad-7, Ad-19, Ad-28 and/or Ad-45 (Andrews et al 1989 ;Nash, 1992 ;Weiss et al 1992 ;Ey et al 1992 ;Mayrhofer et al 1995 ;Monis et al 1996Monis et al , 1999.…”

Section: Alignmentsmentioning

confidence: 99%

Comparative evaluation ofGiardia duodenalissequence data

2007

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A review of the Giardia duodenalis sequences currently available on the GenBank database was completed to compare the different genotyping loci (small subunit ribosomal DNA, glutamate dehydrogenase, triose-phosphate isomerase and beta giardin) for their ability to discern assemblage and subassemblage groups and infer phylogenetic relationships. In total, 405 Giardia duodenalis sequences were sorted and aligned to examine the substitutions within and between the assemblages -A and B (zoonotic), C and D (dogs), E (livestock), F (cats) and G (rodents). It was found that all of the genes could reproducibly group isolates into their assemblages and that the AI/AII subassemblage groups were robust and identifiable at all loci. However, the assemblage B subgroups were not reproducible at half of the loci (small subunit ribosomal DNA and beta giardin), not due to their conserved nature, but because there was insufficient sequence data of reference isolates available for comparison. It is anticipated that further investigation of these loci may reveal the core subgroups of this medically important and zoonotic assemblage and also those of others. The closer, more recent, phylogenetic relationships amongst the assemblages appear to be resolved ; however, more sequence data from the current loci, and possibly new loci, will be required to establish the remaining relationships.

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Giardia intestinalis: Electrophoretic evidence fora species complex

Cited by 137 publications

References 26 publications

Identification of Assemblage A Giardia in White-Tailed Deer

Identification of Assemblage A Giardia in White-Tailed Deer

Cyst morphology and sequence analysis of the small subunit rDNA and ef1α identifies a novel Giardia genotype in a quenda (Isoodon obesulus) from Western Australia

Comparative evaluation ofGiardia duodenalissequence data

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