Giardia duodenalis: Genetic recombination and its implications for taxonomy and molecular epidemiology

Cacciò, Simone M.; Sprong, Hein

doi:10.1016/j.exppara.2009.02.007

Cited by 75 publications

(60 citation statements)

References 36 publications

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“…In the present study, all the assemblage B isolates typed at the gdh locus (n = 13) are considered BIV variants, whereas at the tpi locus, two of the 11 assemblage B isolates were typed as BII, with the remainder BIV. Lack of concordance in the assignment to assemblages and sub-assemblages has been reported in many recent studies (Cacciò et al 2008;Ryan and Cacciò, 2013) and is explained by either mixed infections or recombination (Cacciò and Sprong, 2010). In the first case, two different assemblages/ sub-assemblages are responsible for the infection of a single host and their detection depends on the relative proportion (with the majority population being favoured by PCR) and on the lack of bias during amplification.…”

Section: Discussionmentioning

confidence: 99%

Molecular typing ofGiardia duodenalisin humans in Queensland – first report of Assemblage E

2017

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SUMMARYLittle is known about the genetic diversity of the protozoan parasite, Giardia duodenalis, infecting humans in Queensland, Australia. The present study typed 88 microscopically Giardia-positive isolates using assemblage-specific primers at the triose phosphate isomerase (tpi) gene and sequenced a subset of isolates at the glutamate dehydrogenase (gdh) gene (n = 30) and tpi locus (n = 27). Using the tpi-assemblage specific primers, G. duodenalis assemblage A and assemblage B were detected in 50% (44/88) and 38·6% (34/88) of samples, respectively. Mixed infections with assemblages A and B were identified in 4·5% (4/88) and assemblage E was identified in 6·8% (6/88) of samples. Sequence analysis at the gdh and tpi loci also confirmed the presence of assemblage E in these isolates. Cyst numbers per gram of feces (g−1) were determined using quantitative polymerase chain reaction and of the isolates that were typed as assemblage E, cyst numbers ranged 13·8–68·3 × 106 cysts g−1. This is the first report of assemblage E in humans in Australia, indicating that in certain settings, this assemblage may be zoonotic.

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Section: Discussionmentioning

confidence: 99%

Molecular typing ofGiardia duodenalisin humans in Queensland – first report of Assemblage E

2017

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“…As a matter of fact, it is remarkable that meiosis genes have been identified in organisms that exhibit a typical PCE pattern. They are present in Giardia (Birky, 2005;Cacci o and Sprong, 2010;Heitman, 2006;Lasek-Nesselquist et al, 2009;Ortega-Pierres et al, 2009;Monis et al, 2009;, Leishmania major (Birky, 2005;Heitman, 2006), L. donovani (Birky, 2005), T. cruzi (Heitman, 2006) and T. vivax (Duffy et al, 2009), among others. In Giardia, there is no apparent correlation between the presence or absence of meiotic genes and the observation of meiotic life cycles (Andersson, 2012).…”

Section: Meiosis Genes and Experimental Evolution: What Do They Tell mentioning

confidence: 99%

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

Tibayrenc

Ayala

2017

Advances in Parasitology

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“…intestinalis presents high levels of genetic diversity, and its genotypes are clustered into distinct assemblages. Parasites isolated from humans have been characterized into two assemblages, A and B, both of which are distributed globally [8].…”

Section: Introductionmentioning

confidence: 99%

Giardia intestinalis infection associated with malnutrition in children living in northeastern Brazil

Coronato-Nunes

Calegar

Monteiro

et al. 2017

J Infect Dev Ctries

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Introduction: The present study aimed to determine the prevalence and factors associated with Giardia intestinalis infection, verifying its impact on the nutritional status of children in northeastern Brazil. Methodology: A cross-sectional study was conducted to obtain parasitological, sociodemographic, and anthropometric data in two municipalities in the states of Piauí and Ceará, northeastern Brazil. 3%], p = 0.038, OR = 3.54, 95% CI = 1.13-11.09, respectively). Infection with G. intestinalis remained significantly associated with stunting and being underweight after adjustment for poverty, municipality, sex, and age in a logistic regression multivariate model. Conclusions: In rural areas in northeastern Brazil, giardiasis has acquired great public health importance in the soil-transmitted helminths control era, impacting the nutritional status of children and requiring new approaches to diagnosis and treatment and translational research that could generate applicable solutions at the community level.

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Giardia duodenalis: Genetic recombination and its implications for taxonomy and molecular epidemiology

Cited by 75 publications

References 36 publications

Molecular typing ofGiardia duodenalisin humans in Queensland – first report of Assemblage E

Molecular typing ofGiardia duodenalisin humans in Queensland – first report of Assemblage E

Is Predominant Clonal Evolution a Common Evolutionary Adaptation to Parasitism in Pathogenic Parasitic Protozoa, Fungi, Bacteria, and Viruses?

Giardia intestinalis infection associated with malnutrition in children living in northeastern Brazil

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