Giant Marine Cyanobacteria Produce Exciting Potential Pharmaceuticals

Gerwick, William H.; Coates, Roger; Engene, Niclas; Gerwick, Lena; Grindberg, Rashel V.; Jones, Adam C.; Sorrels, Carla M.

doi:10.1128/microbe.3.277.1

Cited by 37 publications

(48 citation statements)

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“…Identification of secondary metabolites required support of predicted isotope patterns, corresponding tandem MS fragmentations, and conserved retention times (RTs) that were compared with those of previously characterized NPs. Proposed NPs which lacked standards for verifications were isolated by HPLC and characterized by 1 H nuclear magnetic resonance (NMR) and high-resolution ESI-MS. 1 H NMR data were obtained on a JEOL 600-MHz spectrometer. Gene sequencing.…”

Section: Methodsmentioning

confidence: 99%

“…n recent years, tropical and subtropical, benthic marine cyanobacteria have attracted much attention due to their extraordinary capacities to produce structurally diverse and highly bioactive secondary metabolites (1)(2)(3)(4). These bioactive molecules deter grazers, are often potent toxins, and can underlie cyanobacterial harmful algal blooms (CyanoHABs) (5,6).…”

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confidence: 99%

“…Despite their toxicity, many cyanobacterial secondary metabolites also have potential for a broad spectrum of pharmaceutical applications, such as anticancer, anti-inflammatory, antibacterial, and anti-infective applications (1,2,4,9). In addition, many of these cyanobacterial secondary metabolites also have other potential commercial applications, such as insecticides, algaecides, and herbicides (10).…”

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confidence: 99%

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Phylogenetic Inferences Reveal a Large Extent of Novel Biodiversity in Chemically Rich Tropical Marine Cyanobacteria

Engene

Gunasekera

Gerwick

et al. 2013

Appl Environ Microbiol

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bBenthic marine cyanobacteria are known for their prolific biosynthetic capacities to produce structurally diverse secondary metabolites with biomedical application and their ability to form cyanobacterial harmful algal blooms. In an effort to provide taxonomic clarity to better guide future natural product drug discovery investigations and harmful algal bloom monitoring, this study investigated the taxonomy of tropical and subtropical natural product-producing marine cyanobacteria on the basis of their evolutionary relatedness. Our phylogenetic inferences of marine cyanobacterial strains responsible for over 100 bioactive secondary metabolites revealed an uneven taxonomic distribution, with a few groups being responsible for the vast majority of these molecules. Our data also suggest a high degree of novel biodiversity among natural product-producing strains that was previously overlooked by traditional morphology-based taxonomic approaches. This unrecognized biodiversity is primarily due to a lack of proper classification systems since the taxonomy of tropical and subtropical, benthic marine cyanobacteria has only recently been analyzed by phylogenetic methods. This evolutionary study provides a framework for a more robust classification system to better understand the taxonomy of tropical and subtropical marine cyanobacteria and the distribution of natural products in marine cyanobacteria.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Phylogenetic Inferences Reveal a Large Extent of Novel Biodiversity in Chemically Rich Tropical Marine Cyanobacteria

Engene

Gunasekera

Gerwick

et al. 2013

Appl Environ Microbiol

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“…Marine cyanobacteria are vital producers of diverse chemical entities with significant bioactivities [7][8][9][10]. The genus Moorea (formerly Lygnbya) [11] has been proven to be a rich source for novel bioactive compounds of different classes [11,12].…”

Section: Introductionmentioning

confidence: 99%

New Cerebroside and Nucleoside Derivatives from a Red Sea Strain of the Marine Cyanobacterium Moorea producens

et al. 2016

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Abstract:In the course of our ongoing efforts to identify marine-derived bioactive compounds, the marine cyanobacterium Moorea producens was investigated. The organic extract of the Red Sea cyanobacterium afforded one new cerebroside, mooreaside A (1), two new nucleoside derivatives, 3-acetyl-2 1 -deoxyuridine (2) and 3-phenylethyl-2 1 -deoxyuridine (3), along with the previously reported compounds thymidine (4) and 2,3-dihydroxypropyl heptacosanoate (5). The structures of the compounds were determined by different spectroscopic studies (UV, IR, 1D, 2D NMR, and HRESIMS), as well as comparison with the literature data. Compounds 1-5 showed variable cytotoxic activity against three cancer cell lines.

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“…Carmichael and Li (2006) reported the production of microcystins by a marine Synechococcus from Salton Sea, and observed that microcystins may show a more common occurrence in marine environments. Some toxins have promising anticancer, antimycobacterial or other anti-disease activities (Gerwick et al, 2008;Ramos et al, 2015). In our work we detected the microcystins by ELISA technique in the strains of S. aquatilis and Synechocystis spp., and the mcyB gene was detected in only one of these strains.…”

Section: Discussionmentioning

confidence: 88%

Microcystins production and antibacterial activity of cyanobacterial strains of Synechocystis, Synechococcus and Romeria isolated from water and coral reef organisms of Brazilian coast

Barboza

Gorlach‐Lira

Sassi

et al. 2017

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Cyanobacteria are widely distributed in terrestrial, freshwater and marine environments, and over the past decades have been recognized as a powerful source of bioactive compounds. In this study, some cyanobacterial strains were isolated from samples of seawater, brackish water and tissue of reef benthic invertebrates (zoanthid Protopalythoa variabilis, the sponges Cynachrella sp. and Haliclona sp., the coral Siderastrea stellata, and ascidians), collected at the states of Paraíba and Rio Grande do Norte (Northeast of Brazil), during the period between July 2010 and February 2014. After standard isolation methods, the cultivation of the strains was carried out in acclimatized culture chamber (25 °C) under constant aeration, for 15 days at 12-hour photoperiod, using Conway and BG11 media made with filtered seawater. The cyanobacterial cells were analysed for the microcystin production by the ELISA technique and their ethanolic and methanolic extracts for the antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa by the agar well diffusion method. The detection of the mcyB gene, one of the genes related to the microcystin synthesis, was done by the Polymerase Chain Reaction (PCR) technique. The majority of the eighteen cyanobacterial strains belonged to Synechococcaceae Family. The genera of Synechocystis, Synechococcus and Romeria were represented by ten, six and two strains, respectively. The production of microcystins was observed in five strains belonging to the genus Synechocystis. The presence of mcyB gene was detected in 12 strains of cyanobacteria: Synechocystis (three strains), Synechococcus (six strains) and Romeria (two strains). Only one strain (Synechocystis aquatilis) showed both the microcystin production and the mcyB gene presence. The antibacterial activity was observed for one strain of Romeria gracilis, one strain of Synechocystis aquatilis and two strains of Synechococcus sp. The ethanolic extracts of R. gracilis strain and two Synechococcus spp. strains inhibited the growth of P. aeruginosa. Among methanolic extracts of cyanobacteria, only one strain of S. aquatilis showed activity against S. aureus, and one R. gracilis strain against P. aeruginosa. Some cyanobacterial strains studied were positive for the microcystin production and antibacterial activity against pathogenic bacteria S. aureus and P. aeuruginosa, and may be further explored for additional biotechnological applications. Rev. Biol. Trop. 65 (3): 890-899. Epub 2017 September 01.Key words: Synechococcales, extracts, mcyB, cyanobacteria.Cyanobacteria or blue-green algae are photosynthetic prokaryotes widely distributed in almost all habitats, from aquatic marine and freshwater ones to terrestrial environments, being also associated with various marine organisms such as corals and sponges (Glas et al., 2010;Paerl & Paul, 2011).In the last decades, cyanobacteria have been gaining attention in ecology, biochemistry, physiology and molecular biology, because of their high potential for antibiotics and ...

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Giant Marine Cyanobacteria Produce Exciting Potential Pharmaceuticals

Cited by 37 publications

References 10 publications

Phylogenetic Inferences Reveal a Large Extent of Novel Biodiversity in Chemically Rich Tropical Marine Cyanobacteria

Phylogenetic Inferences Reveal a Large Extent of Novel Biodiversity in Chemically Rich Tropical Marine Cyanobacteria

New Cerebroside and Nucleoside Derivatives from a Red Sea Strain of the Marine Cyanobacterium Moorea producens

Microcystins production and antibacterial activity of cyanobacterial strains of Synechocystis, Synechococcus and Romeria isolated from water and coral reef organisms of Brazilian coast

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