Giant magnetoresistive-based biosensing probe station system for multiplex protein assays

Wang, Yi; Wang, Wei; Yu, Lina; Tu, Liang; Feng, Yinglong; Klein, Todd; Wang, Jian Ping

doi:10.1016/j.bios.2015.03.011

Cited by 66 publications

(54 citation statements)

References 40 publications

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“…The values of B ex obtained from VSM measurements and analysis of the sensor field sweeps were in excellent agreement, and were found to be well described by Eq. (8). As B K depended only little on the stack composition, one would expect to observe the highest value ofS 0 for the stack with the largest values of t FM and t Cu .…”

Section: A Effects Of T Cu and T Fm On Sensor Behaviormentioning

confidence: 91%

“…1,2 In these, magnetic beads are usually used as specific labels that bind to the target analyte, and because the biological sample provides no magnetic background signal, the analyte may be detected in real-time with high sensitivity and specificity. [3][4][5][6][7][8] Magnetoresistive biosensors based on the planar Hall effect, 9-11 magnetic tunneling effect, 12,13 or giant magnetoresistance effect [4][5][6] have been proposed for this application. We have previously demonstrated the use of planar Hall effect bridge (PHEB) magnetic field sensors in both volumeand surface-based detection schemes, 7,14,15 and how the sensor design can be optimized towards such diverse applications.…”

Section: Introductionmentioning

confidence: 99%

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Planar Hall effect bridge sensors with NiFe/Cu/IrMn stack optimized for self-field magnetic bead detection

Henriksen

Rizzi

Hansen

2016

Journal of Applied Physics

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Articles you may be interested inThe stack composition in trilayer Planar Hall effect bridge sensors is investigated experimentally to identify the optimal stack for magnetic bead detection using the sensor self-field. The sensors were fabricated using exchange-biased stacks Ni 80 Fe 20 (t FM )/Cu(t Cu )/Mn 80 Ir 20 (10 nm) with t FM ¼ 10, 20, and 30 nm, and 0 t Cu 0.6 nm. The sensors were characterized by magnetic hysteresis measurements, by measurements of the sensor response vs. applied field, and by measurements of the sensor response to a suspension of magnetic beads magnetized by the sensor self-field due to the sensor bias current. The exchange bias field was found to decay exponentially with t Cu and inversely with t FM . The reduced exchange field for larger values of t FM and t Cu resulted in higher sensitivities to both magnetic fields and magnetic beads. We argue that the maximum magnetic bead signal is limited by Joule heating of the sensors and, thus, that the magnetic stacks should be compared at constant power consumption. For a fixed sensor geometry, the figure of merit for this comparison is the magnetic field sensitivity normalized by the sensor bias voltage. In this regard, we found that sensors with t FM ¼ 20 nm or 30 nm outperformed those with t FM ¼ 10 nm by a factor of approximately two, because the latter have a reduced AMR ratio. Further, the optimum layer thicknesses, t Cu % 0.6 nm and t FM ¼ 20-30 nm, gave a 90% higher signal compared to the corresponding sensors with t Cu ¼ 0 nm. V C 2016 AIP Publishing LLC.

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Section: A Effects Of T Cu and T Fm On Sensor Behaviormentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Planar Hall effect bridge sensors with NiFe/Cu/IrMn stack optimized for self-field magnetic bead detection

Henriksen

Rizzi

Hansen

2016

Journal of Applied Physics

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“…100 μL of 2 μg/mL IFNγ B-dAb solution was pipetted into the chip surface and incubated for 1 hour at room temperature, and then the chip was washed three times with PBST before being connected into tester system. GMR chips were tested and measured by a probe system with minor modification [5]. After GMR chip was loaded and running for a few minutes to stabilize all sensors, 80 μL of magnetic nanoparticles (MNPs) solution was added.…”

Section: Methodsmentioning

confidence: 99%

Nanomagnetic Biosensor for the Detection of Porcine Interferon Gamma

Wang

Aboubakr

Vang

et al. 2017

2017 Design of Medical Devices Conference

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Due to the anatomical and physiological similarities to humans that include similar heart size, flow rate, skin, liver enzymes and bone healing, porcine models as a powerful investigational platform have been widely used in research areas such as diabetes, obesity and islet transplantation [1]. The advantages of relative low cost, ease in handling and comparatively short period of breeding time may make swine provide a promising solution to the shortage of human donors and difficulty in isolating purified islets from adult human in future. Porcine cytokines play a significant role in innate immunity, apoptosis, angiogenesis, cell growth and differentiation. They are involved in cellular responses, maintenance of homeostasis, and disease states such as inflammatory disease, cardiovascular disease, and cancer. Thus, the technologies to analyze the expression of cytokines are developed rapidly and are still hot topics. The traditional approach for cytokine detection and quantification is the use of an enzyme-linked immunosorbent assay (ELISA). However, its inability to do multiplex test calls for more robust detection system. Biochip-based assay for the detection of biological agents using giant magnetoresistive (GMR) sensors and magnetic nanoparticles have emerged recently [2, 3]. It is proved that the nanomagnetic biosensor technology has advantages of low cost, high sensitivity, multiplexity, and real-time signal readout. The integration of GMR biosensor and use of weak magnetic fields allow to eventually realize point-of-care and portability. In addition, interferon gamma (IFNγ) is one of the most important porcine cytokines, and is associated with a number of autoinflammatory and autoimmune diseases. In this work, IFNγ is selected as a model target for the detection of porcine cytokine using nanomagnetic GMR biosensor.

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“…For the past two decades, magnetic biosensors have received considerable attention as they offer several key advantages over conventional and competing sensing methods456789101112131415161718. Like an ELISA, a magnetic immunoassay (MIA) relies on two antibodies that form a sandwich structure around the biomarker of interest to achieve high specificity.…”

mentioning

confidence: 99%

Giant Magnetoresistive Biosensors for Time-Domain Magnetorelaxometry: A Theoretical Investigation and Progress Toward an Immunoassay

Huang

Zhou

Hall

2017

Sci Rep

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Magnetorelaxometry (MRX) is a promising new biosensing technique for point-of-care diagnostics. Historically, magnetic sensors have been primarily used to monitor the stray field of magnetic nanoparticles bound to analytes of interest for immunoassays and flow cytometers. In MRX, the magnetic nanoparticles (MNPs) are first magnetized and then the temporal response is monitored after removing the magnetic field. This new sensing modality is insensitive to the magnetic field homogeneity making it more amenable to low-power portable applications. In this work, we systematically investigated time-domain MRX by measuring the signal dependence on the applied field, magnetization time, and magnetic core size. The extracted characteristic times varied for different magnetic MNPs, exhibiting unique magnetic signatures. We also measured the signal contribution based on the MNP location and correlated the coverage with measured signal amplitude. Lastly, we demonstrated, for the first time, a GMR-based time-domain MRX bioassay. This approach validates the feasibility of immunoassays using GMR-based MRX and provides an alternative platform for point-of-care diagnostics.

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Giant magnetoresistive-based biosensing probe station system for multiplex protein assays

Cited by 66 publications

References 40 publications

Planar Hall effect bridge sensors with NiFe/Cu/IrMn stack optimized for self-field magnetic bead detection

Planar Hall effect bridge sensors with NiFe/Cu/IrMn stack optimized for self-field magnetic bead detection

Nanomagnetic Biosensor for the Detection of Porcine Interferon Gamma

Giant Magnetoresistive Biosensors for Time-Domain Magnetorelaxometry: A Theoretical Investigation and Progress Toward an Immunoassay

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