Giant Amphiphiles by Cofactor Reconstitution

Boerakker, MJ Mark; Hannink, JM; Bomans, Phh Paul; Frederik, PM Peter; Nolte, Roeland J. M.; Meijer, EM Emmo; Sommerdijk, Nico A. J. M.

doi:10.1002/1521-3773(20021115)41:22<4239::aid-anie4239>3.0.co;2-e

Cited by 144 publications

(111 citation statements)

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“…Whether the membrane has a mono-or a bilayered structure, and whether the PEG and HRP are segregated or mixed in the corona, is not yet known. The enzyme-polymer hybrid vesicles were further investigated according to their enzymatic activity [95,96]. The enzymatic activity decreased for HRP and the stability of the oxy complex was reduced for Mb.…”

Section: Formation Of Polymersomes From Polymers Comprising Biofunctimentioning

confidence: 99%

Functionalization of Block Copolymer Vesicle Surfaces

et al. 2011

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Abstract:In dilute aqueous solutions certain amphiphilic block copolymers self-assemble into vesicles that enclose a small pool of water with a membrane. Such polymersomes have promising applications ranging from targeted drug-delivery devices, to biosensors, and nanoreactors. Interactions between block copolymer membranes and their surroundings are important factors that determine their potential biomedical applications. Such interactions are influenced predominantly by the membrane surface. We review methods to functionalize block copolymer vesicle surfaces by chemical means with ligands such as antibodies, adhesion moieties, enzymes, carbohydrates and fluorophores. Furthermore, surface-functionalization can be achieved by self-assembly of polymers that carry ligands at their chain ends or in their hydrophilic blocks. While this review focuses on the strategies to functionalize vesicle surfaces, the applications realized by, and envisioned for, such functional polymersomes are also highlighted.

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Section: Formation Of Polymersomes From Polymers Comprising Biofunctimentioning

confidence: 99%

Functionalization of Block Copolymer Vesicle Surfaces

et al. 2011

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“…Boerakker et al have developed such a biohybrid amphiphile by reconstituting apo-horseradish peroxidase (apo-HRP) with its cofactor, ferriprotoporphyrin IX, carrying a polystyrene tail (Figure 24a and b). 230 In water, the enzymepolymer hybrid formed vesicular aggregates with diameters of 80-400 nm (Figure 24c).…”

Section: Polymersomes As Nanoreactorsmentioning

confidence: 99%

“…230 Velonia et al have constructed another type of biohybrid amphiphile by specific attachment of a polystyrene block to a reduced disulfide bridge of CAL B, exposed on the outer surface of the enzyme. 254 TEM studies of aqueous dispersions of PS-CAL B giant amphiphiles revealed the presence of micrometer long fibers that were built up from bundles of micellar rods.…”

Section: Polymer Micelles As Nanoreactorsmentioning

confidence: 99%

Self‐Assembled Nanoreactors

et al. 2005

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“…[16][17][18][19][20][21][22][23][24][25][26] Examples include polymer conjugation via: co-factor reconstitution, 16 the biotin-streptavidin complex, 18 supramolecular complexation with cucurbituril, 19 charge-charge 20 and metal affinity 21,24 interactions, as well as lectin-mediated complexation. 25,27,28 The advantage of noncovalent systems is that temporal control of conjugate assembly and disassembly can be asserted by the presence of competitor molecules.…”

Section: Introductionmentioning

confidence: 99%

Noncovalent PEGylation via Lectin–Glycopolymer Interactions

et al. 2016

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The full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 2 AbstractPEGylation, the covalent modification of proteins with polyethylene glycol, is an abundantly used technique to improve the pharmacokinetics of therapeutic proteins. The drawback with this methodology is that the covalently attached PEG can impede the biological activity (e.g. reduced receptor-binding capacity). Protein therapeutics with "disposable" PEG modifiers have potential advantages over the current technology. Here, we show that a protein-polymer "Medusa complex" is formed by the combination of a hexavalent lectin with a glycopolymer. Using NMR spectroscopy, small angle X-ray scattering (SAXS), size exclusion chromatography and native gel electrophoresis it was demonstrated that the fucose-binding lectin RSL and a fucose-capped polyethylene glycol (Fuc-PEG) form a multimeric assembly. All of the experimental methods provided evidence of noncovalent PEGylation with a concomitant increase in molecular mass and hydrodynamic radius. The affinity of the protein-polymer complex was determined by ITCand competition experiments to be in the µM range, suggesting that such systems have potential biomedical applications.

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Giant Amphiphiles by Cofactor Reconstitution

Abstract: Controlled modification of horseradish peroxidase with an apolar polymer chain (see figure) by cofactor reconstitution leads to giant amphiphiles, which form vesicular aggregates in aqueous solution.

Cited by 144 publications

References 1 publication

Functionalization of Block Copolymer Vesicle Surfaces

Functionalization of Block Copolymer Vesicle Surfaces

Self‐Assembled Nanoreactors

Noncovalent PEGylation via Lectin–Glycopolymer Interactions

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