GhTULP34, a member of tubby-like proteins, interacts with GhSKP1A to negatively regulate plant osmotic stress

Li, Zhanshuai; Wang, Xiaoyan; Cao, Xiaocong; Chen, Baizhi; Ma, Changkai; Lv, Jiaoyan; Sun, Zhimao; Qiao, Kaikai; Zhu, Longfu; Zhang, Chaojun; Fan, Shuli; Ma, Qin

doi:10.1016/j.ygeno.2020.09.024

Cited by 24 publications

(28 citation statements)

References 68 publications

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“…Transcriptome analysis has shown that cotton GhTULPs are involved in abiotic stresses and tissue development. Overexpression of GhTULP34 was shown to decrease the germination rate of Arabidopsis seeds under salt stress, inhibit root development under osmotic stress, and lead to the closure of plant stomata ( Li Z. et al, 2021 ). In summary, TLPs play key roles in plant growth and development and in responses to biotic and abiotic stresses.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses

Liu

et al. 2022

Front. Plant Sci.

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Tubby-like proteins (TLPs) are transcription factors that are widely present in eukaryotes and generally participate in growth and developmental processes. Using genome databases, a total of 22 putative TLP genes were identified in the soybean genome, and unevenly distributed across 13 chromosomes. Phylogenetic analysis demonstrated that the predicted GmTLP proteins were divided into five groups (I-V). Gene structure, protein motifs, and conserved domains were analyzed to identify differences and common features among the GmTLPs. A three-dimensional protein model was built to show the typical structure of TLPs. Analysis of publicly available gene expression data showed that GmTLP genes were differentially expressed in response to abiotic stresses. Based on those data, GmTLP8 was selected to further explore the role of TLPs in soybean drought and salt stress responses. GmTLP8 overexpressors had improved tolerance to drought and salt stresses, whereas the opposite was true of GmTLP8-RNAi lines. 3,3-diaminobenzidine and nitro blue tetrazolium staining and physiological indexes also showed that overexpression of GmTLP8 enhanced the tolerance of soybean to drought and salt stresses; in addition, downstream stress-responsive genes were upregulated in response to drought and salt stresses. This study provides new insights into the function of GmTLPs in response to abiotic stresses.

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Section: Introductionmentioning

confidence: 99%

Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses

Liu

et al. 2022

Front. Plant Sci.

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“…We are interested in the evolution of the cotton TLP gene family and their possible roles in abiotic stress responses. So far, only a single member of the family GhTULP34 has been characterized (Li et al, 2020 ). In this study, we have carried out comprehensive genomic exploration of TLP protein family in G. raimondii, G. arboreum, G. barbadense , and G. hirsutum and studied the expression profiles of G. hirsutum TLPs ( GhTLPs ) in salt and drought stress responses.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Evolutionary Analysis of Gossypium Tubby-Like Protein (TLP) Gene Family and Expression Analyses During Salt and Drought Stress

et al. 2021

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Tubby-like proteins (TLPs) possess a highly conserved closed β barrel tubby domain at C-terminal and N-terminal F-box. The role of TLP gene family members has been widely discussed in numerous organisms; however, the detailed genome-wide study of this gene family in Gossypium species has not been reported till date. Here, we systematically identified 105 TLP gene family members in cotton (Gossypium arboreum, Gossypium raimondii, Gossypium hirsutum, and Gossypium barbadense) genomes and classified them into eight phylogenetic groups. Cotton TLP12 gene family members clustered into two groups, 4 and 8. They experienced higher evolutionary pressure in comparison to others, indicating the faster evolution in both diploid as well as in tetraploid cotton. Cotton TLP gene family members expanded mainly due to segmental duplication, while only one pair of tandem duplication was found in cotton TLPs paralogous gene pairs. Subsequent qRT-PCR validation of seven putative key candidate genes of GhTLPs indicated that GhTLP11A and GhTLP12A.1 genes were highly sensitive to salt and drought stress. The co-expression network, pathways, and cis-regulatory elements of GhTLP11A and GhTLP12A.1 genes confirmed their functional importance in salt and drought stress responses. This study proposes the significance of GhTLP11A and GhTLP12A.1 genes in exerting control over salt and drought stress responses in G. hirsutum and also provides a reference for future research, elaborating the biological roles of G. hirsutum TLPs in both stress responses.

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“…In salt stress conditions, the WRKY6 expression was found to be enhanced in response to ROS treatment and it also triggers other plant defense responses and senescence processes ( Robatzek and Somssich, 2001 ; Li et al, 2020 ). The member of EARLY FLOWERING3 ( ELF ) have an important role in providing tolerant to high NaCl ( Sakuraba et al, 2017 ; Cheng et al, 2020 ) and Tubby-like protein (TLP) members have been identified to play a crucial role in several abiotic responses such as drought, salt, oxidative ( Wardhan et al, 2012 ; Xu et al, 2016 ; Li Z. S. et al, 2021 ). Furthermore, the expression of expansin-like B1 ( EXPB1 ) was found to be upregulated by salt, oxidative, and osmotic conditions Brassica rapa ( Muthusamy et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…The higher expression of TLP1 in Cicer arietinum led to increased tolerance to salt, drought, and oxidative stress in tobacco ( Wardhan et al, 2012 ). Moreover, AtTLP3 member has been found to respond to abiotic stresses ( Li Z. S. et al, 2021 ). The overall outcomes suggested that NSP2, DRE1D, TLP5, and TLP3 in drought and TLP1, WRKY6, ATHB7, and EF109 in salt may be crucial in cotton improvement.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Meta-Analysis Associated Targeting Hub Genes and Pathways of Drought and Salt Stress Responses in Cotton (Gossypium hirsutum): A Network Biology Approach

et al. 2022

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Abiotic stress tolerance is an intricate feature controlled through several genes and networks in the plant system. In abiotic stress, salt, and drought are well known to limit cotton productivity. Transcriptomics meta-analysis has arisen as a robust method to unravel the stress-responsive molecular network in crops. In order to understand drought and salt stress tolerance mechanisms, a meta-analysis of transcriptome studies is crucial. To confront these issues, here, we have given details of genes and networks associated with significant differential expression in response to salt and drought stress. The key regulatory hub genes of drought and salt stress conditions have notable associations with functional drought and salt stress-responsive (DSSR) genes. In the network study, nodulation signaling pathways 2 (NSP2), Dehydration-responsive element1 D (DRE1D), ethylene response factor (ERF61), cycling DOF factor 1 (CDF1), and tubby like protein 3 (TLP3) genes in drought and tubby like protein 1 (TLP1), thaumatin-like proteins (TLP), ethylene-responsive transcription factor ERF109 (EF109), ETS-Related transcription Factor (ELF4), and Arabidopsis thaliana homeodomain leucine-zipper gene (ATHB7) genes in salt showed the significant putative functions and pathways related to providing tolerance against drought and salt stress conditions along with the significant expression values. These outcomes provide potential candidate genes for further in-depth functional studies in cotton, which could be useful for the selection of an improved genotype of Gossypium hirsutum against drought and salt stress conditions.

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GhTULP34, a member of tubby-like proteins, interacts with GhSKP1A to negatively regulate plant osmotic stress

Cited by 24 publications

References 68 publications

Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses

Comprehensive Profiling of Tubby-Like Proteins in Soybean and Roles of the GmTLP8 Gene in Abiotic Stress Responses

Genome-Wide Identification and Evolutionary Analysis of Gossypium Tubby-Like Protein (TLP) Gene Family and Expression Analyses During Salt and Drought Stress

Transcriptome Meta-Analysis Associated Targeting Hub Genes and Pathways of Drought and Salt Stress Responses in Cotton (Gossypium hirsutum): A Network Biology Approach

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