GHRH|HaeIII Gene Polymorphism in Dairy and Beef Cattle at National Livestock Breeding Centers

Abstract

ABSTRACT), kecuali pada sapi FH di BIB Lembang, sapi Brahman, dan Angus di BET Cipelang. Nilai heterozigositas tertinggi adalah 0,471 untuk sapi FH pejantan di BIB Lembang, sebaliknya, nilai terendah adalah 0,344 untuk sapi FH pejantan di BBIB Singosari. Nilai heterozigositas sapi FH lebih rendah daripada sapi Simmental dan Limousin. Gen GHRH pada sapi FH dan sapi pedaging menunjukkan polimorfik, kecuali pada sapi Brahman yang hanya memiliki alel B.

Association between GH (g.1456_1457insT), GHRH (g.4474 C>A), and Pit-1 (g.244G>A) Polymorphisms and Lactation Traits in Holstein Friesian Cattle

“…The GHRH|HaeIII locus or the GHRH g.4474 C>A SNP re-sulted in three genotypes, i.e., AA genotype by 3 bands (312, 94, and 45 bp), CC genotype by 4 bands (194, 118, 94, and 45 bp), and AC genotype by 5 bands (312, 194, 118, 94, and 45 bp). These three genotypes were consistent to the results reported by previous study (Rini et al, 2013).…”

“…The same results were reported on this genetic polymorphism of HF cattle from the two AI centers and an ET Center providing a very rare of the AA (4.5%) cattle than the AC cattle (37.1%) and CC cattle (58.4%); likewise generating a very low A allele (22.5%) rather than C allele (77.5%) (Rini et al, 2013). Some previous studies also confirmed the same results for the lowest AA animals compared to the AB (AC) and BB (CC) animals, such as in Polish Holstein i.e., 7.8% vs. 33.9% and 58.3%; abd Jersey i.e., 3.2% vs. 42.7% and 54.1% (Szatkowska et al, 2009).…”

“…Amplified DNA product of the GHRH gene produced the base fragment by 451 bp, located in exon 2, intron 2, and exon 3 of this gene. This fragment fitted to the genome library (GenBank: AF242855) as previously reported by Rini et al (2013). The HaeIII restriction enzyme recognized the cutting base fragment at the nucleotide sites of 4472, 4666, and 4760 or at the base positions of 118, 312, and 406 of the PCR products.…”

“…Specific restriction enzymes used to cut each of GH, GHRH and Pit-1 genes were successively MspI, HaeIII, and HinfI. The MspI enzyme recognizes base sites of C*CGG or GH g.1456_1457 InsT (Dybus, 2002), the HaeIII enzyme recognizes base sites of GG*CC or GHRH g.4474 C>A SNP (Rini et al, 2013), and HinfI enzyme recognizes base sites of G*ANTC or Pit-1 g.244 G>A SNP (Dybus et al, 2004).…”

Effect of Growth Hormone-releasing Hormone Gene Polymorphism (GHRH/HAEIII) on First Lactation Milk Yield and Milk Composition Traits in Sahiwal Cattle

“…Similar frequencies of rare GHRH A allele, 0.2480 and 0.2400 were reported by Dybus and Grzestak (2006) and Klauzinska et al (2004), respectively. Further, frequencies for the GHRH A and GHRH B allele vary considerably between breeds, GHRH A allele was found to be lowest in cattle breeds such as the Hereford (Moody et al, 1995), Holstein (Kmiec et al, 2007;Szatkowska et al, 2009;Rini et al, 2013), Jersey (Szatkowska et al, 2009), Simmental and Limousin (Rini et al, 2013;Dybus et al, 2003). In contrast to those breeds, GHRH A allele frequency was found to highest in Angus cattle (Moody et al,1995;Rini et al, 2013), whereas the GHRH B allele was found with a maximum frequency in Brahman breed which originates from Bos indicus (Rini et al, 2013).…”