Ghrelin in mental health, sleep, memory

Steiger, Axel; Dresler, Martin; Schüssler, P.; Kluge, Michael

doi:10.1016/j.mce.2011.02.013

Cited by 62 publications

(49 citation statements)

References 81 publications

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“…Total RNA was isolated using TRIzol (Invitrogen); one-colour gene expression analysis was performed following Agilent labelling (Quick Amp Labeling Kit), hybridisation and scanning (Agilent Feature Extraction version 10.5.1) protocols using the custom Agilent 2!105K microarray. Data were normalised using the quantile normalisation method in Linear Models for Microarray Data (LIMMA), an open-source R-based Software package for the analysis of gene expression data (Smyth 2005). Differential gene expression was determined using LIMMA linear models with an false discovery rate (FDR)-adjusted P value %0.05 and fold change R1.5.…”

Section: Microarray Profiling Of Insulin-treated Lncap Prostate Cancementioning

confidence: 99%

Cloning of a novel insulin-regulated ghrelin transcript in prostate cancer

Seim¹,

Lubik²,

Lehman³

et al. 2012

Journal of Molecular Endocrinology

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Ghrelin is a multifunctional hormone, with roles in stimulating appetite and regulating energy balance, insulin secretion and glucose homoeostasis. The ghrelin gene locus (GHRL) is highly complex and gives rise to a range of novel transcripts derived from alternative first exons and internally spliced exons. The wild-type transcript encodes a 117 amino acid preprohormone that is processed to yield the 28 amino acid peptide ghrelin. Here, we identified insulin-responsive transcription corresponding to cryptic exons in intron 2 of the human ghrelin gene. A transcript, termed in2c-ghrelin (intron 2-cryptic), was cloned from the testis and the LNCaP prostate cancer cell line. This transcript may encode an 83 amino acid preproghrelin isoform that codes for ghrelin, but not obestatin. It is expressed in a limited number of normal tissues and in tumours of the prostate, testis, breast and ovary. Finally, we confirmed that in2c-ghrelin transcript expression, as well as the recently described in1-ghrelin transcript, is significantly upregulated by insulin in cultured prostate cancer cells. Metabolic syndrome and hyperinsulinaemia have been associated with prostate cancer risk and progression. This may be particularly significant after androgen deprivation therapy for prostate cancer, which induces hyperinsulinaemia, and this could contribute to castrate-resistant prostate cancer growth. We have previously demonstrated that ghrelin stimulates prostate cancer cell line proliferation in vitro. This study is the first description of insulin regulation of a ghrelin transcript in cancer and should provide further impetus for studies into the expression, regulation and function of ghrelin gene products.

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Section: Microarray Profiling Of Insulin-treated Lncap Prostate Cancementioning

confidence: 99%

Cloning of a novel insulin-regulated ghrelin transcript in prostate cancer

Seim¹,

Lubik²,

Lehman³

et al. 2012

Journal of Molecular Endocrinology

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“…By contrast, other studies suggested that rising ghrelin would contribute to the development of stress-induced depression and anxiety (Asakawa et al, 2001a;Carlini et al, 2002Carlini et al, , 2004Carvajal et al, 2009). Furthermore, increasing evidence indicates that ghrelin not only plays a role in anxiety and stress, but it is also involved in promoting learning behaviour and memory processes and in sleep-wake regulation (Steiger et al, 2011). In particular, a sleep-promoting potential of ghrelin has been supported (Steiger et al, 2011).…”

Section: Ghrelin Regulation and Functionsmentioning

confidence: 99%

“…Furthermore, increasing evidence indicates that ghrelin not only plays a role in anxiety and stress, but it is also involved in promoting learning behaviour and memory processes and in sleep-wake regulation (Steiger et al, 2011). In particular, a sleep-promoting potential of ghrelin has been supported (Steiger et al, 2011). Szentirmai and co-workers reported that ghrelin knock out rodents sleep less than wild type ones (Szentirmai et al, 2007).…”

Section: Ghrelin Regulation and Functionsmentioning

confidence: 99%

“…It also influences glucose and insulin metabolism and controls food and energy intake through many neuroendocrine systems (van der . Furthermore, several evidences suggest that ghrelin not only plays a metabolic role but it is also involved in sleepwake regulation, affective status, learning and memory processes (Steiger et al, 2011;van der Lely et al, 2004). Besides, the recent discovery of ghrelin has also provided an important insight to the neuroendocrine knowledge in epilepsy.…”

Section: Introductionmentioning

confidence: 99%

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Ghrelin Regulation in Epilepsy

Prodam¹,

Bellone²,

Ricotti³

et al. 2011

Underlying Mechanisms of Epilepsy

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“…Also, these studies have focused mainly on major depression cases [1][2][3] not chronic or depression resulting from mild environmental stresses. So, studies on endocrine indices of various models of depression are necessary [4] . Among trusty models of depression, chronic mild stress (CMS) model of depression in rodents has been proposed to model some of the environmental factors that contribute to the induction of depressive disorders in humans [5][6][7] .…”

Section: Introductionmentioning

confidence: 99%

Investigation on Serum Hormonal Parameters (Ghrelin, Corticosterone, Insulin, T3 and T4) in Chronic Mild Stress Rat Model of Depression

Narimani-Rad¹,

Babapour²,

Zendehdel³

et al. 2014

Kafkas Univ Vet Fak Derg

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The aim of present study was to investigate the effects of depression induced with chronic mild stress (CMS) protocol on serum hormones (ghrelin, corticosterone, insulin, T 3 and T 4 ) which is in interaction with stress or depressive disorders. Forty-five 40-45 d old laboratory rats were assigned to two groups; control (n: 10) and CMS (subjected to CMS procedure, n: 30). Rats in the control group were reared in single cages without any environmental stress. Rats in CMS were entered the CMS procedure. This protocol consisted of mild unpredictable stressors (intermittent illumination, stroboscopic light, grouping, food or water deprivation, exposure to an empty water bottle, solid cage, cage tilting, etc.). After CMS protocol sucrose preference (SP) test was used for the identification of depressed animals. Rats with lower than 65% SP were defined as depressed animal. Blood serum was taken from two groups (control and CMS) for the determination of blood hormonal variables by Elisa kits. Analyzed data showed a significant elevation in ghrelin, corticosterone, and insulin levels for CMS group (depressed animals) in comparison with control group, as elevation was very significant for corticosterone. There was not any significant change for T 3 , whereas decreased T 4 was observed for CMS groups when compared with the control. It was concluded that chronic mild stress induced depression can cause ghrelin, corticosteroid, and insulin increases, and T 4 decreases. Mentioned effects of CMS induced depression on hormonal indices (with exception to thyroid hormones), can be similar with effects of major depression. Keywords: Chronic mild stress, Depression, Glucocorticoids, Insulin Depresyonun Kronik Hafif Stres Sıçan Modelinde SerumHormonal Parametrelerin (Ghrelin, Kortikosteron, İnsülin, T 3 ve T 4 ) Araştırılması ÖzetBu çalışmada, depresyon etkilerinin kronik hafif stres (CMS) işlemi ile stres ve depresif bozukluklarla etkileşim halinde olan serum hormonları (ghrelin, kortikosteron, insülin, T 3 ve T 4 ) üzerine etkilerinin araştırılması amaçlanmıştır. Kırk beş adet 40-45 günlük laboratuvar sıçanları kontrol (n: 10) ve CMS (CMS işlemine tabi, n: 30) olmak üzere iki gruba ayrıldı. Kontrol grubundaki sıçanlar herhangi bir çevresel stres olmadan tek bir kafeste yetiştirildi. CMS grubu sıçanlar CMS işlemine dahil edildi. Bu protokol hafif öngörülemeyen stres oluşturucularının (kesintili aydınlatma, stroboskopik ışık, gruplama, yiyecek veya su yoksunluğu, boş bir su şişesine, katı kafese, kafes eğimine maruziyet, vb) oluşmaktadır. CMS işleminden sonra sükroz tercih (SP) testi depresif hayvanların tanımlanması için kullanıldı. %65 SP'den daha düşük sıçanlar depresif hayvan olarak tanımlandı. Kan serumu ELISA kitleri ile kan hormonsal değişkenlerin tespiti için iki grutan (kontrol ve CMS) alındı. Analiz edilen sonuçlar kontrol grubu ile karşılaştırıldığında çok önemli kortikosteron artışıyla beraber CMS grupta (depresif hayvanlar) ghrelin, kortikosteron ve insülin seviyelerindeki önemli artışı gösterdi. CMS gruplarında kontro...

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Ghrelin in mental health, sleep, memory

Cited by 62 publications

References 81 publications

Cloning of a novel insulin-regulated ghrelin transcript in prostate cancer

Cloning of a novel insulin-regulated ghrelin transcript in prostate cancer

Ghrelin Regulation in Epilepsy

Investigation on Serum Hormonal Parameters (Ghrelin, Corticosterone, Insulin, T3 and T4) in Chronic Mild Stress Rat Model of Depression

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