Ghrelin and Leptin Did Not Improve Meiotic Maturation of Porcine Oocytes Cultured<i>In Vitro</i>

Suzuki, Hiroyuki; Sasaki, Yukie; Shimizu, Masayo; Matsuzaki, Masatoshi; Hashizume, Takumi; Kuwayama, Hideto

doi:10.1111/j.1439-0531.2009.01352.x

Cited by 11 publications

(14 citation statements)

References 13 publications

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“…These observations confirm previous reports (Xia et al, 1994;Illera et al, 1998;Sirotkin et al, 2000) of stimulatory action of IGF-I on porcine oocyte nuclear maturation. However, they do not correspond to the previous reports of stimulatory action (Craig et al, 2005;Kun et al, 2007) or lack of effect (Jin et al, 2009;Suzuki et al, 2009) of leptin on porcine oocyte maturation. Furthermore, they do not confirm a previous report (Suzuki et al, 2009) of inhibitory action of ghrelin on this process.…”

Section: In Immatured Oocytescontrasting

confidence: 99%

“…However, they do not correspond to the previous reports of stimulatory action (Craig et al, 2005;Kun et al, 2007) or lack of effect (Jin et al, 2009;Suzuki et al, 2009) of leptin on porcine oocyte maturation. Furthermore, they do not confirm a previous report (Suzuki et al, 2009) of inhibitory action of ghrelin on this process. This is the first demonstration to show leptin can not only promote or influence oocyte maturation, but also it can inhibit oocyte maturation.…”

Section: In Immatured Oocytescontrasting

confidence: 99%

“…Some authors (Craig et al, 2005;Kun et al, 2007) have reported the promotion of meiosis in porcine oocytes after leptin addition. Other authors (Jin et al, 2009;Suzuki et al, 2009), however, did not observe any effect of leptin on this process. Slight inhibition of porcine oocyte nuclear maturation has been observed after ghrelin addition (Suzuki et al, 2009).…”

Section: Introductionmentioning

confidence: 81%

“…Other authors (Jin et al, 2009;Suzuki et al, 2009), however, did not observe any effect of leptin on this process. Slight inhibition of porcine oocyte nuclear maturation has been observed after ghrelin addition (Suzuki et al, 2009). Obestatin effect on oocyte maturation has not been examined as yet.…”

Section: Introductionmentioning

confidence: 81%

See 3 more Smart Citations

Involvement of the metabolic hormones leptin, ghrelin, obestatin, IGF-I and of MAP kinase in control of porcine oocyte maturation

Sirotkin

Bezakova

Laurinčík

et al. 2011

Animal

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The general aim of our in vitro experiments was to study the role of the metabolic hormones leptin, ghrelin, obestatin and IGF-I and mitogen-activated protein kinase (MAPK)-dependent intracellular mechanisms in the control of nuclear maturation of porcine oocytes. For this purpose, porcine oocytes were isolated from the ovary and cultured in the presence of leptin, ghrelin, obestatin, IGF-I, MAPK blocker PD98059 and the combinations of hormones with PD98059. Proportions of matured oocytes (at metaphase II of meiosis, determined by DAPI staining) and of oocytes containing MAPK/ERK1-2 (determined by immunocytochemistry) were measured before and after culture. It was observed that the majority of oocytes isolated from the ovary before culture were immature and did not contain visible MAPK, but some oocytes were mature, and the majority of these oocytes contained MAPK. Incubation of oocytes resulted in a significant increase in the proportion of matured oocytes and in the percentage of oocytes containing MAPK in both the matured and not matured groups. Addition of IGF-I to the culture medium increased the proportion of matured oocytes, addition of leptin decreased it, and ghrelin and obestatin did not oocyte maturation. Addition of hormones did not affect the expression of MAPK in either immature or mature oocytes. PD98059, when given alone, suppressed the maturation and accumulation of MAPK in both mature and immature oocytes. When given together with hormones, PD98059 was able to reduce the stimulatory effect of IGF-I, to invert the inhibitory action of leptin to stimulatory and to induce the stimulatory action of ghrelin and obestatin on meiosis. IGF-I, ghrelin and obestatin, but not leptin, when given together with PD98059, increased the accumulation of MAPK in both immature and mature oocytes. Association of nuclear maturation and expression of MAPK in oocytes before, but not after culture, as well as the prevention of oocyte maturation by MAPK blocker suggests the involvement of MAPK-dependent intracellular mechanisms in the promotion of reinitiation, but not completion of meiosis. The effect of hormonal additions on meiosis of oocytes suggests that IGF-I is a stimulator, leptin can be an inhibitor, while ghrelin and obestatin probably do not control oocyte maturation. The ability of PD98059 to modify the effect of hormones on oocyte maturation and on MAPK expression suggests possible interference of hormones and MAPK-dependent intracellular mechanisms in oocytes. However, no influence of hormones on MAPK and lack of association between action of hormones and PD98059 on MAPK and meiosis suggest that MAPK is probably not a mediator of effect of IGF-I, leptin, ghrelin and obestatin on porcine oocyte nuclear maturation. Keywords: leptin, ghrelin, obestatin, MAP kinase, oocyte Implication These data demonstrated the involvement of metabolic hormones and mitogen-activated protein kinase (MAPK)-dependent intracellular mechanisms in the control of oocyte nuclear maturation. This expands the existing knowledge conc...

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Section: In Immatured Oocytescontrasting

confidence: 99%

Section: In Immatured Oocytescontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 81%

See 2 more Smart Citations

Involvement of the metabolic hormones leptin, ghrelin, obestatin, IGF-I and of MAP kinase in control of porcine oocyte maturation

Sirotkin

Bezakova

Laurinčík

et al. 2011

Animal

View full text Add to dashboard Cite

show abstract

“…However, controversial results have been reported in the literature. In some species, leptin seems to promote rearrangement of cytoskeletal elements (Suzuki et al 2010) that are involved in chromosome segregation and in processes of organelle movement (Sun & Schatten 2006). Other studies have reported that leptin enhances an appropriate spindle assembly during metaphase (Jin et al 2009) and stimulates meiotic oocyte maturation in mouse (Matsuoka et al 1999), pig (Craig et al 2004, Kun et al 2007, Zhang et al 2007) and cattle (Paula-Lopes et al 2007, Van Tol et al 2008.…”

Section: Discussionmentioning

confidence: 99%

Influence of leptin on in vitro maturation and steroidogenic secretion of cumulus–oocyte complexes through JAK2/STAT3 and MEK 1/2 pathways in the rabbit model

Arias-Álvarez¹,

García-García²,

Torres-Rovira³

et al. 2010

Reproduction

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Extreme body mass indexes may impair reproductive outcome in assisted reproductive technologies. Leptin reflects the amount of body fat and could act as a modulator of oocyte quality through activation of specific transcription factors. The aim of this work was to establish whether: 1) leptin influences meiotic and cytoplasmic oocyte maturation; 2) STAT3 and MAPK mediate the effects of leptin and 3) leptin modulates steroid secretion by cumulus-oocyte complexes (COC) during in vitro maturation (IVM). We confirmed immunolocalisation of leptin receptor in oocytes, cumulus/granulosa cells during the peri-ovulatory period. The confocal study showed that COC supplemented with 1, 10 and 100 ng/ml leptin had a significantly higher metaphase II (MII) percentage than those IVM without leptin (P!0.05) and a similar MII index compared to the group supplemented with 10% FCS. Leptin did not increase the percentage of cytoplasmically matured oocytes in terms of cortical granule migration rate, whereas a significantly higher index was found in the FCS group (P!0.001). Oestradiol concentrations in spent media were higher in the FCS group compared to other treatments (P!0.001). Leptin-stimulated nuclear oocyte maturation was significantly impaired when leptin-induced JAK2/STAT3 and MEK 1/2 activation was suppressed by the inhibitors (P!0.001). Steroid secretion of COC was not affected by leptin activation of JAK2/STAT3 or MEK 1/2 pathways. In conclusion, JAK2/STAT3 and MEK 1/2 pathways mediate the enhancement of nuclear oocyte maturation by leptin; however, neither cytoplasmic oocyte maturation nor steroidogenic response of COC were improved in the present rabbit model.

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The effect of leptin on maturing porcine oocytes is dependent on glucose concentration

Silva

Paczkowski

Krisher

2012

Molecular Reproduction Devel

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Increased body weight is often accompanied by increased circulating levels of leptin and glucose, which alters glucose metabolism in various tissues, including perhaps the oocyte. Alteration of glucose metabolism impacts oocyte function and may contribute to the subfertility often associated with obese individuals. The objective of this study was to determine the effect of leptin (0, 10, and 100 ng/ml) on the oocyte and cumulus cells during in vitro maturation under differing glucose concentrations. We examined the effects of leptin on oocyte maturation, blastocyst development, and/or gene expression in oocytes and cumulus cells (IRS1, IGF1, PPARγ, IL6, GLUT1) in a physiological glucose (2 mM) and high glucose (50 mM) environment. We also evaluated the effect of leptin on glucose metabolism via glycolysis and the pentose phosphate pathway. In a physiological glucose environment, leptin did not have an influence on oocyte maturation, blastocyst development, or oocyte gene expression. Expression of GLUT1 in cumulus cells was downregulated with 100 ng/ml leptin treatment, but did not affect oocyte glucose metabolism. In a high glucose environment, oocyte maturation and glycolysis were decreased, but in the presence of 100 ng/ml leptin, these parameters were improved to levels similar to control. This effect is potentially mediated by an upregulation of oocyte IRS1 and a correction of cumulus cell IGF1 expression. The present study demonstrates that in a physiological glucose concentration, leptin plays a negligible role in oocyte function. However, leptin appears to modulate the deleterious impact of a high glucose environment on oocyte function.

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Ghrelin and Leptin Did Not Improve Meiotic Maturation of Porcine Oocytes CulturedIn Vitro

Cited by 11 publications

References 13 publications

Involvement of the metabolic hormones leptin, ghrelin, obestatin, IGF-I and of MAP kinase in control of porcine oocyte maturation

Involvement of the metabolic hormones leptin, ghrelin, obestatin, IGF-I and of MAP kinase in control of porcine oocyte maturation

Influence of leptin on in vitro maturation and steroidogenic secretion of cumulus–oocyte complexes through JAK2/STAT3 and MEK 1/2 pathways in the rabbit model

The effect of leptin on maturing porcine oocytes is dependent on glucose concentration

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