Ghrelin and cell differentiation

Xu, Geyang; Li, Yin; An, Wenjiao; Zhang, Weizhen

doi:10.1111/j.1745-7270.2008.00465.x

Cited by 6 publications

(2 citation statements)

References 62 publications

(56 reference statements)

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“…The reason led to such difference is that the open pore produced unavoidable in the preparation process because the Carbon fiber braid can't be densified completely by SiC matrix. Before the glass fiber removed by hydrofluoric acid, the composites have original open porosity about 4 percent after 16 pyrolysis cycle (Table 1) [6]. To prove the result that the increment of open porosity was led by the removed glass fiber, the X-ray diffraction analysis of the composites was done (Fig.…”

Section: Resultsmentioning

confidence: 95%

Fabrication of Porous Transpiration C/SiC Composites

Zhang

Zhou

et al. 2008

KEM

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Section: Resultsmentioning

confidence: 95%

Fabrication of Porous Transpiration C/SiC Composites

Zhang

Zhou

et al. 2008

KEM

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“…For example, its contribution has been reported in recovery and synaptic formation at the hippocampal level (Berrout and Isokawa 2012;Stoyanova et al 2013;Kent et al 2015). Moreover, emerging evidence indicates that ghrelin can also stimulate the proliferation, differentiation and migration of neural stem/progenitor cells (Xu et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Ghrelin peptide improves glial conditioned medium effects on neuronal differentiation of human adipose mesenchymal stem cells

Russo

Mannino

Patané

et al. 2021

Histochem Cell Biol

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The influences of ghrelin on neural differentiation of adipose-derived mesenchymal stem cells (ASCs) were investigated in this study. The expression of typical neuronal markers, such as protein gene product 9.5 (PGP9.5) and Microtubule Associated Protein 2 (MAP2), as well as glial Fibrillary Acid Protein (GFAP) as a glial marker was evaluated in ASCs in different conditions. In particular, 2 µM ghrelin was added to control ASCs and to ASCs undergoing neural differentiation. For this purpose, ASCs were cultured in Conditioned Media obtained from Olfactory Ensheathing cells (OEC-CM) or from Schwann cells (SC-CM). Data on marker expression were gathered after 1 and 7 days of culture by fluorescence immunocytochemistry and flow cytometry. Results show that only weak effects were induced by the addition of only ghrelin. Instead, dynamic ghrelin-induced modifications were detected on the increased marker expression elicited by glial conditioned media. In fact, the combination of ghrelin and conditioned media consistently induced a further increase of PGP9.5 and MAP2 expression, especially after 7 days of treatment. The combination of ghrelin with SC-CM produced the most evident effects. Weak or no modifications were found on conditioned medium-induced GFAP increases. Observations on the ghrelin receptor indicate that its expression in control ASCs, virtually unchanged by the addition of only ghrelin, was considerably increased by CM treatment. These increases were enhanced by combining ghrelin and CM treatment, especially at 7 days. Overall, it can be assumed that ghrelin favors a neuronal rather than a glial ASC differentiation.

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Ghrelin accelerates the cartilagic differentiation of rabbit mesenchymal stem cells through the ERK1/2 pathway

Wang

Dou

et al. 2017

Cytotechnology

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Mesenchymal stem cells (MSCs) can differentiate into chondroblasts, adipocytes, or cartilage under appropriate stimulation. Identifying a mechanism triggering the differentiation of MSCs into cartilage may help develop novel therapeutic approaches for treating heterotopic ossification, the pathological formation of lamellar bone in soft tissue outside the skeleton that can lead to debilitating immobility. Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, stimulates growth hormone secretion, and has both orexigenic and adipogenic effects. This study sought to understand the potential involvement of the ERK1/2 signaling pathway in the ghrelin-induced growth of rat MSCs (rMSCs). We applied various concentrations of ghrelin to cultured rMSCs by observing the changes in the phosphorylation state of ERK1/2, p38, JNK as well as the type II collagen expression levels by western blot. The highest expression level for both type II collagen was obtained with 600 ng/mL ghrelin at 24 h. We found that the ghrelin-induced differentiation of rMSCs into cartilage was promoted primarily by the ERK1/2 pathway. Our study suggests that ghrelin induced differentiation of rMSCs into cartilage primarily through the ERK1/2 pathway.

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Ghrelin and cell differentiation

Cited by 6 publications

References 62 publications

Fabrication of Porous Transpiration C/SiC Composites

Fabrication of Porous Transpiration C/SiC Composites

Ghrelin peptide improves glial conditioned medium effects on neuronal differentiation of human adipose mesenchymal stem cells

Ghrelin accelerates the cartilagic differentiation of rabbit mesenchymal stem cells through the ERK1/2 pathway

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