GhGLK1 a Key Candidate Gene From GARP Family Enhances Cold and Drought Stress Tolerance in Cotton

Abstract

Drought and low-temperature stresses are the most prominent abiotic stresses affecting cotton. Wild cotton being exposed to harsh environments has more potential to cope with both biotic and abiotic stresses. Exploiting wild cotton material to induce resistant germplasm would be of greater interest. The candidate gene was identified in the BC2F2 population among Gossypium tomentosum and Gossypium hirsutum as wild male donor parent noted for its drought tolerance and the recurrent parent and a high yielding but drought susceptible species by genotyping by sequencing (GBS) mapping. Golden2-like (GLK) gene, which belongs to the GARP family, is a kind of plant-specific transcription factor (TF) that was silenced by virus-induced gene silencing (VIGS). Silencing of GhGLK1 in cotton results in more damage to plants under drought and cold stress as compared with wild type (WT). The overexpression of GhGLK1 in Arabidopsis thaliana showed that the overexpressing plants showed more adaptability than the WT after drought and cold treatments. The results of trypan blue and 3,3′-diaminobenzidine (DAB) staining showed that after drought and cold treatment, the leaf damage in GhGLK1 overexpressed plants was less as compared with the WT, and the ion permeability was also lower. This study suggested that the GhGLK1 gene may be involved in the regulation of drought and cold stress response in cotton. Our current research findings add significantly to the existing knowledge of cold and drought stress tolerance in cotton.

Protoplast Dissociation and Transcriptome Analysis Provides Insights to Salt Stress Response in Cotton

“…Total RNA was extracted with an RNAprep Pure Plant Kit (Tiangen, Beijing, China) from root samples or the protoplasts, following the instructions of the manufacturer. The quality and the concentration of each RNA sample were determined by NanoDrop 2000 spectrophotometer and RNA that fulfilled the standard at 260/280 in a range of 1.80–2.1 was used for further analysis [ 47 ]. The RNA quality was also determined by agarose gel electrophoresis and spectrometric analysis.…”

Identification and Analysis of MYB Gene Family for Discovering Potential Regulators Responding to Abiotic Stresses in Curcuma wenyujin

“…There are two viewpoints about GARP-like proteins: 1) those that belong to MYB -related subfamily due to a similar motif but have distant relationships; and 2) those that do not belong to MYB -related subfamily due to the only “W” residue in conserved MYB DBD ( Du et al, 2013 ; Safi et al, 2017 ). Arabidopsis overexpressing GhGLK1 (a GARP -like gene) showed more adaptability to drought and cold treatments with the less leaf damage and lower ion permeability ( Liu et al, 2021 ). Interestingly, R-R type MYB-related proteins were the exception, with two far apart “R” located in the N-terminal and middle of the sequence, respectively.…”

Identification of GOLDEN2-like transcription factor genes in soybeans and their role in regulating plant development and metal ion stresses

“…AtGLK1 increases resistance to Fusarium graminearum in Arabidopsis and promotes cucumber mosaic virus tolerance (Savitch et al, 2007;Schreiber et al, 2011;Han et al, 2016). AhGLK1b can enhance tolerance to fungal and bacterial infections and other environmental stresses (Liu et al, 2021b). In rice, OsGLK1 is involved in disease resistance (Chen et al, 2016).…”

“…Loss offunction of SlGLK29 may affect cold tolerance in tomato plants (Junfang et al, 2017). Similarly, GhGLK1 in cotton has been associated with the response to cold and drought stress (Liu et al, 2021b). Several GLK gene functions have been studied extensively.…”