GGNBP2 Suppresses the Proliferation, Invasion, and Migration of Human Glioma Cells

Zhan, Aiyun; Lei, Bo; Wu, Honggang; Ye, Wen; Zheng, Liandong; Wang, Shan; Wan, Xiaoqiang; Wei, Zhenghong

doi:10.3727/096504016x14816726393937

Cited by 13 publications

(17 citation statements)

References 9 publications

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“…Prostate cancer is the most common malignant tumour in men of developed countries and the second leading cause for death among male cancer patients. GGNBP2 is located in the human chromosome 17q12‐21.1 region, and the genes encoded in this region are closely related to the progression of breast cancer (Yang et al, ), ovarian cancer (May et al, ), laryngeal cancer (Chen et al, ) and glioma (Zhan et al, ). In tumour cells, the normal processes of gene transcription and cleavage are disrupted, and abnormal mutations in genes and disorders in transcription and translation processes can lead to the synthesis of mRNA that does not exist originally, or the proportion of transcription or translation of different cuts.…”

Section: Discussionmentioning

confidence: 99%

Effects of gametogenetin‐binding protein 2 on proliferation, invasion and migration of prostate cancer PC‐3 cells

Zhou

Wang

2019

Andrologia

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We aimed to assess the effects of gametogenetin‐binding protein 2 (GGNBP2) on the proliferation, invasion and migration of prostate cancer PC‐3 cells. PcDNA3‐HisC‐GGNBP2 was transfected to overexpress GGNBP2. Proliferation was tested by MTT assay, and migration and invasion were detected by Transwell assay. Cell cycle was detected by flow cytometry. The protein expressions of COX‐2, cyclin D1, PI3K, Akt and p‐Akt were detected by Western blot. A subcutaneous xenograft model of prostate cancer was established. Mice were randomly divided into three groups (n = 9) and intratumorally injected with pcDNA3‐HisC‐GGNBP2, pcDNA3‐HisC and normal saline respectively. The xenograft tumour volume was measured every 3 days, and weight was measured after 2 weeks. After GGNBP2 overexpression, the proliferation, migration and invasion capacities of PC‐3 cells decreased, and cell cycle was arrested in the G1 phase. The protein expressions of COX‐2, cyclin D1, PI3K, Akt and p‐Akt all reduced. The tumour volume and weight of pcDNA3‐HisC‐GGNBP2 group were significantly lower than those of pcDNA3‐HisC group (p < .05). The proliferation capacity of GGNBP2‐overexpressing prostate cancer cells is significantly attenuated, tumour growth is significantly inhibited, and cell cycle is arrested in the G1 phase. GGNBP2 overexpression affects the growth of castration‐resistant prostate cancer via the PI3K/Akt signalling pathway.

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Section: Discussionmentioning

confidence: 99%

Effects of gametogenetin‐binding protein 2 on proliferation, invasion and migration of prostate cancer PC‐3 cells

Zhou

Wang

2019

Andrologia

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“…ZFP403 is closely associated with the occurrence and development of several types of cancer ( 13 ). Studies have demonstrated that full-length ZFP403 may serve as a potential tumor suppressor, and that ZFP403 is downregulated in various malignant tumors, such as breast ( 9 ), ovarian cancer ( 14 ) and glioma ( 15 ). Furthermore, the absence of ZFP403 can promote cellular proliferation ( 16 ) and tumorigenesis.…”

Section: Introductionmentioning

confidence: 99%

Effects of zinc finger protein 403 on the proliferation, migration and invasion abilities of prostate cancer cells

Zhu

et al. 2020

Oncol Rep

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Zinc finger protein 403 ( ZFP403 ), located on human chromosome 17q12-21, is closely associated with the development of cancer. However, to date, there are a limited number of studies on the biological functions of this gene, particularly in prostate cancer (PCa). The results of the present study demonstrated that compared with normal tissues, the expression of ZFP403 was markedly lower in PCa tissues, as shown by the evaluation of the Gene Expression Profiling Interactive Analysis 2 database. The decreased expression of ZFP403 in PCa clinical tissues and cell lines was confirmed by immunohistochemistry, reverse transcription-quantitative PCR and western blot analysis. Using short harpin (sh)RNA inhibition, stably-silenced ZFP403 cell lines were then constructed by lentiviral transfection (LV-PC3-shRNA-1 and 2; LV-DU145-shRNA-1 and 2). The results revealed that the knockdown of ZFP403 in PCa cells promoted cellular proliferation, colony formation, migration and invasiveness in vitro . Moreover, the levels of tumor growth- and motility-related proteins were significantly altered after ZFP403 -knockdown. A xenograft tumor model using nude mice was established to elucidate the role of ZFP403 in tumorigenesis in vivo . Tumor growth was significantly increased in mice injected with ZFP403 -knockdown cells compared with the control mice. Overall, the findings of the present study demonstrate that ZFP403 functions as a tumor suppressor gene in PCa by affecting the proliferation, migration and invasiveness of PCa cells, suggesting its potential use as a clinical diagnostic marker.

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“…Furthermore, overexpression of GGNBP2 suppresses tumor proliferation and metastasis and its down-regulation is associated with drug resistance in ovarian cancer [11,12]. In glioma, GGNBP2 also functions a tumor suppressor [13]. However, some studies indicate that depletion of GGNBP2 leads to cell cycle arrest at G2/M phase and inhibition of cell proliferation, suggesting that GGNBP2 may also function as an oncogene [14].…”

Section: Introductionmentioning

confidence: 99%

GGNBP2 suppresses triple-negative breast cancer aggressiveness through inhibition of IL-6/STAT3 signaling activation

Liu

Yagüe

et al. 2018

Breast Cancer Res Treat

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Triple negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, lacking effective targeted therapies, and whose underlying mechanisms are still unclear. The gene coding for Gametogenetin-binding Protein (GGNBP2), also known as Zinc Finger Protein 403 (ZNF403), is located on chromosome 17q12-q23, a region known as a breast cancer susceptibility locus. We have previously reported that GGNBP2 functions as a tumor suppressor in estrogen receptor-positive breast cancer. In this study we find that GGNBP2 expression decreases in TNBC tissues and is associated with the outcome of breast cancer patients. Furthermore, experimental overexpression of GGNBP2 in MDA-MB-231 and Cal51 cells suppresses cell proliferation, migration and invasion, reduces the cancer stem cell subpopulation and promotes cell apoptosis in vitro as well as inhibits tumor growth in vivo. In these cell models, overexpression of GGNBP2 decreases the activation of IL-6/STAT3 signaling. Thus, GGNBP2 suppresses cancer progression by inhibition of IL-6/STAT3 activation in TNBC.

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GGNBP2 Suppresses the Proliferation, Invasion, and Migration of Human Glioma Cells

Cited by 13 publications

References 9 publications

Effects of gametogenetin‐binding protein 2 on proliferation, invasion and migration of prostate cancer PC‐3 cells

Effects of gametogenetin‐binding protein 2 on proliferation, invasion and migration of prostate cancer PC‐3 cells

Effects of zinc finger protein 403 on the proliferation, migration and invasion abilities of prostate cancer cells

GGNBP2 suppresses triple-negative breast cancer aggressiveness through inhibition of IL-6/STAT3 signaling activation

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