gga-miR-101-3p Plays a Key Role in Mycoplasma gallisepticum (HS Strain) Infection of Chicken

Abstract

Mycoplasma gallisepticum (MG), one of the most pathogenic Mycoplasma, has caused tremendous economic loss in the poultry industry. Recently, increasing evidence has suggested that micro ribonucleic acids (miRNAs) are involved in microbial pathogenesis. However, little is known about potential roles of miRNAs in MG infection of chicken. In the present study, using miRNA Solexa sequencing we have found that gga-miR-101-3p was up-regulated in the lungs of MG-infected chicken embryos. Moreover, gga-miR-101-3p regulated expression of the host enhancer of zeste homolog 2 (EZH2) through binding to the 3’ un-translated region (3’-UTR) of EZH2 gene. Over-expression of gga-miR-101-3p significantly inhibited EZH2 expression and hence inhibited proliferation of chicken embryonic fibroblast (DF-1 cells) by blocking the G1-to-S phase transition. Similar results were obtained in MG-infected chicken embryos and DF-1 cells, where gga-miR-101-3p was significantly up-regulated, while EZH2 was significantly down-regulated. This study reveals that gga-miR-101-3p plays an important role in MG infection through regulation of EZH2 expression and provides a new insight into the mechanisms of MG pathogenesis.

Identification of differentially expressed miRNAs through high-throughput sequencing in the chicken lung in response to Mycoplasma gallisepticum HS

“…A previous study from our group showed that gga-miR-101-3p played an important role in MG infection (Chen et al, 2015). miR-101 regulates activation of MAPKs in macrophages to defend against microbial infection (Zhu et al, 2010).…”

gga-miR-451 Negatively Regulates Mycoplasma gallisepticum (HS Strain)-Induced Inflammatory Cytokine Production via Targeting YWHAZ

“…A large amount of evidence has demonstrated that miRNAs are involved in cellular behaviors with the aim of resisting or promoting inflammation during infections and inflammation in mammals [ 36 , 37 ]. Recently, we also reported the effects of gga-miR-19a, gga-miR-99a, and gga-miR-101-3p in MG-mediated inflammation in chicken [ 24 , 25 , 38 ]. However, the role of miRNAs in the MG-induced respiratory inflammation is largely unknown.…”

“…Other miRNAs, including miR-16 and miR-29a, are reported to participate in the protumoral inflammatory process by activating the TLR8 response on immune cells [ 15 ]. Recently, we also reported the role of gga-miR-101 and gga-miR-19a in regulating MG-HS infection and MG-HS-mediated inflammatory cytokine production in both DF-1 cells and the lungs of chicken embryos [ 24 , 25 ].…”

Research Progress on MicroRNAs Involved in the Regulation of Chicken Diseases

“…GO, KEGG, miRNA-GO-network, path-net, and gene-net analyses determined that the altered miRNAs regulated a large number of genes that function throughout various signaling pathways including the MAPK pathway, the focal adhesion regulatory pathways, the Wnt pathway, and the JAK/STAT pathway (Zhao et al, 2017b). gga-miR-101-3p was found to be up-regulated in the lungs of MG-infected chicken embryos, and gga-miR-101-3p could target and inhibit the host EZH2 (enhancer of zeste homolog 2), a growth suppressor gene involved in the regulation of cell cycle progression and proliferation, to suppress the proliferation of chicken embryonic fibroblasts by blocking the G1-to-S phase transition (Chen et al, 2015). Additionally, SMARCA5 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily A, member 5) is a member of the SWI/SNF family that exhibits ATPase and helicase activities.…”