GFP synthesis in giant liposomes using the in vitro translation system of Thermococcus kodakaraensis

Yamaji, Kazuaki; Kanai, Tamotsu; Nomura, Shin Ichiro M.; Akiyoshi, Kazunari; Negishi, Michiharu; Atomi, Haruyuki; Yoshikawa, Kenichi; Imanaka, Tadayuki

doi:10.1109/mhs.2009.5351893

Cited by 1 publication

(1 citation statement)

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“…The GVs (size 1–100 μm), encapsulating a TX–TL mixture, were monitored in time via confocal microscopy ( Figure 4c ). The amount of produced GFP was variable (CV ∼ 35%), irrespective from the nature and concentration of the template molecule ( c DNA from 3 pM to 30 nM, c RNA from 25 nM to 1 μM) ( 65 , 70 ).…”

Section: A Survey Of Published Resultsmentioning

confidence: 99%

Extrinsic stochastic factors (solute partition) in gene expression inside lipid vesicles and lipid-stabilized water-in-oil droplets: a review

et al. 2018

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The encapsulation of transcription–translation (TX–TL) machinery inside lipid vesicles and water-in-oil droplets leads to the construction of cytomimetic systems (often called ‘synthetic cells’) for synthetic biology and origins-of-life research. A number of recent reports have shown that protein synthesis inside these microcompartments is highly diverse in terms of rate and amount of synthesized protein. Here, we discuss the role of extrinsic stochastic effects (i.e. solute partition phenomena) as relevant factors contributing to this pattern. We evidence and discuss cases where between-compartment diversity seems to exceed the expected theoretical values. The need of accurate determination of solute content inside individual vesicles or droplets is emphasized, aiming at validating or rejecting the predictions calculated from the standard fluctuations theory. At the same time, we promote the integration of experiments and stochastic modeling to reveal the details of solute encapsulation and intra-compartment reactions.

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Section: A Survey Of Published Resultsmentioning

confidence: 99%