GFAP and PCNA Marking in the cerebellum of masu salmon’s (Oncorhynchus masou) juvenile after mechanical injury

Stukaneva, M. E.; Пущина, Е. В.; Вараксин, А. А.

doi:10.1134/s1062360417050071

Cited by 4 publications

(11 citation statements)

References 37 publications

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“…According to data of Zupanc and Ott (1999), BrdU labels a limited number of cells in the S-phase of the cell cycle. The results of studies on O. masou cerebellum indicate that the population of BrdU-labeled cells is more homogeneous than the cells labeled with PCNA (Stukaneva et al, 2017). As the studies on trout (Pushchina et al, 2016) and O. masou (Stukaneva et al, 2017) has shown, the number of PCNA+ cells in the DMZ of cerebellum after a direct damage to the cerebellum (Stukaneva et al, 2017) and to the optic nerve (Pushchina et al, 2016) is much larger than the number of BrdU+ cells after a damage to the cerebellum and telencephalon of O. masou (present data).…”

Section: Discussionmentioning

confidence: 99%

“…PCNA+ cells were found in the periventricular zone of diencephalon, mesencephalic tegmentum, and rhomboencephalon (Pushchina et al, 2012). Studies based on labeling of PCNA showed a high intensity of cell proliferation in the dorsal matrix zone (DMZ) of the cerebellum in juvenile O. masou (Stukaneva et al, 2017; Pushchina et al, 2018). This zone is located in the dorsomedial part of the cerebellum’s body, at the boundary between the molecular and granular layers.…”

Section: Introductionmentioning

confidence: 99%

“…In the dorsal, lateral, and basal zones of the molecular layer of cerebellum in O. masou , neurogenic niches containing PCNA+ cells and a heterogeneous population of PCNA cells were identified after injury (Stukaneva et al, 2017). The appearance of reactive neurogenic niches after a damaging impact is a feature of reparative neurogenesis in fish brain (Than-Trong and Bally-Cuif, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…A study of damage to the telencephalon of adult D. rerio has shown that persistent inflammation does not spread widely, and the glial scar does not develop (Baumgart et al, 2012). However, in the cerebellum of O. masou juveniles, radial glial fibers and single small cells, intensely and moderately labeled with glial fibrillary acidic protein (GFAP), were found after damage (Stukaneva et al, 2017). As a result of the damaging impact, GFAP+ radial glia fibers appeared in the dorsal matrix zone of cerebellum, forming multidirectional, radially oriented beams (Pushchina et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…No similar structural formations were detected in intact O. masou juveniles. After the cerebellum injury in juvenile O. masou , the dorsal matrix zone undergoes a structural rearrangement associated with a partial spatial reorientation of the radial glia fibers and the formation of specific guides for the cells formed in this zone (Stukaneva et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Damage-related changes in the cerebellum of juvenileOncorhynchus masou: reactivation of neurogenic niches and astrocytic response

Пущина

Stukaneva

Вараксин

2019

Preprint

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10In the cerebellum of juvenile Oncorhynchus masou, proliferating BrdU+ and HuCD+ cells and 11 constitutive neurogenic niches were detected in different zones; the largest number of labeled 12 cells were found in the dorsal part of the molecular layer and the dorsal matrix zone (DMZ). 13Cells labeled with glutamine synthetase (GS) and radial glia were also present in the intact O. 14 masou cerebellum. The most intensive proliferation was detected in the rostral part of 15 cerebellum. This part is assumed to contain active zones of constitutive neurogenesis. After an 16 injury inflicted to the cerebellum, the number of BrdU+ and HuCD+ cells increased 17 significantly. The number of BrdU+ cells after this type of injury was much greater than after a 18 telencephalon trauma. A quantitative analysis revealed that after the cerebellum injury the 19 proliferative activity in the caudal part of CCb is increased compared to that in the control. A 20 reactivation of neurogenic and neuroepithelial niches and their transformation into reactive 21 neurogenic domains, with an increased distribution density of intensely labeled HuCD+ cells of 22 different types, were observed. The increase in the number of HuCD+ differentiated cells in the 23 basal area suggests that the processes of neuronal differentiation are intensified in the cerebellum 24 of juvenile O. masou after injury. The number of GS positive cells (GS+) and fibers increased in 25 all the zones of cerebellum. The most intensive astrocytic response was noted in the dorsal part 2 26 of cerebellum. The data of the enzyme immunoassay confirm the multiple variations in the level 27 of GS after a traumatic injury to cerebellum in O. masou. 28

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Damage-related changes in the cerebellum of juvenileOncorhynchus masou: reactivation of neurogenic niches and astrocytic response

Пущина

Stukaneva

Вараксин

2019

Preprint

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Proliferation and Neuro- and Gliogenesis in Normal and Mechanically Damaged Mesencephalic Tegmentum in Juvenile Chum Salmon, Oncorhynchus keta

2019

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Hydrogen Sulfide Modulates Adult and Reparative Neurogenesis in the Cerebellum of Juvenile Masu Salmon, Oncorhynchus masou

Пущина¹,

Stukaneva²,

Вараксин³

2020

IJMS

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Fish are a convenient model for the study of reparative and post-traumatic processes of central nervous system (CNS) recovery, because the formation of new cells in their CNS continues throughout life. After a traumatic injury to the cerebellum of juvenile masu salmon, Oncorhynchus masou, the cell composition of the neurogenic zones containing neural stem cells (NSCs)/neural progenitor cells (NPCs) in the acute period (two days post-injury) changes. The presence of neuroepithelial (NE) and radial glial (RG) neuronal precursors located in the dorsal, lateral, and basal zones of the cerebellar body was shown by the immunohistochemical (IHC) labeling of glutamine synthetase (GS). Progenitors of both types are sources of neurons in the cerebellum of juvenile O. masou during constitutive growth, thus, playing an important role in CNS homeostasis and neuronal plasticity during ontogenesis. Precursors with the RG phenotype were found in the same regions of the molecular layer as part of heterogeneous constitutive neurogenic niches. The presence of neuroepithelial and radial glia GS+ cells indicates a certain proportion of embryonic and adult progenitors and, obviously, different contributions of these cells to constitutive and reparative neurogenesis in the acute post-traumatic period. Expression of nestin and vimentin was revealed in neuroepithelial cerebellar progenitors of juvenile O. masou. Patterns of granular expression of these markers were found in neurogenic niches and adjacent areas, which probably indicates the neurotrophic and proneurogenic effects of vimentin and nestin in constitutive and post-traumatic neurogenesis and a high level of constructive metabolism. No expression of vimentin and nestin was detected in the cerebellar RG of juvenile O. masou. Thus, the molecular markers of NSCs/NPCs in the cerebellum of juvenile O. masou are as follows: vimentin, nestin, and glutamine synthetase label NE cells in intact animals and in the post-traumatic period, while GS expression is present in the RG of intact animals and decreases in the acute post-traumatic period. A study of distribution of cystathionine β-synthase (CBS) in the cerebellum of intact young O. masou showed the expression of the marker mainly in type 1 cells, corresponding to NSCs/NCPs for other molecular markers. In the post-traumatic period, the number of CBS+ cells sharply increased, which indicates the involvement of H2S in the post-traumatic response. Induction of CBS in type 3 cells indicates the involvement of H2S in the metabolism of extracellular glutamate in the cerebellum, a decrease in the production of reactive oxygen species, and also arrest of the oxidative stress development, a weakening of the toxic effects of glutamate, and a reduction in excitotoxicity. The obtained results allow us to consider H2S as a biologically active substance, the numerous known effects of which can be supplemented by participation in the processes of constitutive neurogenesis and neuronal regeneration.

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GFAP and PCNA Marking in the cerebellum of masu salmon’s (Oncorhynchus masou) juvenile after mechanical injury

Cited by 4 publications

References 37 publications

Damage-related changes in the cerebellum of juvenileOncorhynchus masou: reactivation of neurogenic niches and astrocytic response

Damage-related changes in the cerebellum of juvenileOncorhynchus masou: reactivation of neurogenic niches and astrocytic response

Proliferation and Neuro- and Gliogenesis in Normal and Mechanically Damaged Mesencephalic Tegmentum in Juvenile Chum Salmon, Oncorhynchus keta

Hydrogen Sulfide Modulates Adult and Reparative Neurogenesis in the Cerebellum of Juvenile Masu Salmon, Oncorhynchus masou

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