21A variety of tools have emerged with the goal of mapping the current delivery of ecosystem services 22 and quantifying the impact of environmental changes. An important and often overlooked question 23 is how accurate the outputs of these models are in relation to empirical observations. In this paper 24 we validate a hydrological ecosystem service model (InVEST Water Yield Model) using widely 25 available data. We modelled annual water yield in 22 UK catchments with widely varying land cover, 26 population and geology, and compared model outputs with gauged river flow data from the UK 27 National River Flow Archive. Values for input parameters were selected from existing literature to 28 reflect conditions in the UK and were subjected to sensitivity analyses. We also compared model 29 performance between precipitation and potential evapotranspiration data sourced from global-and 30 UK-scale datasets. We then tested the transferability of the results within the UK by additional 31 validation in a further 20 catchments. 32Whilst the model performed only moderately with global-scale data (linear regression of modelled 33 total water yield against empirical data; slope = 0.763, intercept = 54.45, R 2 = 0.963) with wide 34 variation in performance between catchments, the model performed much better when using UK-35 scale input data, with closer fit to the observed data (slope = 1.07, intercept = 3.07, R 2 = 0.990). With 36 UK data the majority of catchments showed less than 10% difference between measured and 37 modelled water yield but there was a minor but consistent overestimate per hectare (86 38 m 3 /ha/year). Additional validation on a further 20 UK catchments was similarly robust, indicating 39 that these results are transferable within the UK. These results suggest that relatively simple 40 models can give accurate measures of ecosystem services. However, the choice of input data is 41 critical and there is a need for further validation in other parts of the world. 42Keywords 43 UK, mapping, rainfall, evapotranspiration, river flow, land cover 44 45