Getting to the root of grafting-induced traits

Williams<, B>; Ahsan, Muhammad Umair; Frank, Margaret H.

doi:10.1016/j.pbi.2020.101988

Cited by 25 publications

(21 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The grafting process is complex, involving a sequential set of changes, from molecular to tissue levels, at the graft interface during graft healing [5,6]. During grafting, there is an abrupt interruption in the exchange of substances between rootstock and scion, resulting in an asymmetry that affects the tissue development and physiological status of both graft partners.…”

Section: Introductionmentioning

confidence: 99%

Tomato Graft Union Failure Is Associated with Alterations in Tissue Development and the Onset of Cell Wall Defense Responses

et al. 2021

View full text Add to dashboard Cite

Grafting is a technique applied to a considerable number of crops, with tomato standing out. However, this technique is limited by the obtaining of unfunctional grafts, which decrease the success rate and therefore the benefits achieved. The aim of this work was to analyze the failure in intraspecific grafting of tomato plants, focusing on tissue development, cell wall defense reactions, and the distribution of starch and soluble sugars at the graft junction. The success rate in autografts was higher than that of homografts and heterografts. Unfunctional homografts and heterografts showed similar responses: absence of vascular reconnections and lack of adhesion between scion and rootstock, even though callus cell clusters and differentiation of new vasculature were produced. The scions of unfunctional grafts accumulated more starch and soluble sugars than the rootstocks, showing a strong asymmetry in the response. In addition, three types of deposits were observed in the cell walls of unfunctional grafts: lignin, suberin, and callose, with the combined accumulation of more than one of them being frequent, particularly lignin and suberin. These deposits apparently prevent adhesion and seem to be a major cause of graft failure.

show abstract

Section: Introductionmentioning

confidence: 99%

Tomato Graft Union Failure Is Associated with Alterations in Tissue Development and the Onset of Cell Wall Defense Responses

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Studies on grafting using different plant species show that signs of incompatibility can occur in the early or late phase, identified between three to five years after grafting (Williams et al, 2021). P. tuberculatum showed initial incompatibility with 'Bragantina' black pepper, caused by the absence of connection at the grafting point, with graft elimination over time.…”

Section: Discussionmentioning

confidence: 99%

Primary metabolism and initial development of grafted black pepper seedlings

Crasque¹,

Arantes²,

Neto³

et al. 2021

RSD

View full text Add to dashboard Cite

The production of black pepper seedlings through grafting is a promising strategy to mitigate the effect of environmental stresses, such as drought, that limit its cultivation. However, studies on compatibility between grafts and rootstocks are still scarce, especially considering the wild species of the genus Piper and the cultivated genotypes of black pepper. The objective of this work was to evaluate the relationship between primary metabolism and the development of black pepper seedlings obtained through intra- and interspecific grafting. The method used was that of cleft lateral grafting, as graft the Bragantina cultivar, better known in the world as ‘Panniyur 1’, and ‘Kottanadan’ cultivar and three wild species (Piper hispidum, Piper aduncum and Piper tuberculatum) were used as root-stock. As a control, ´Bragantina` was grafted onto itself (homograft). The experiment was carried out for 110 days and the seedlings were evaluated for development, gas exchange, and carbohydrate allocation. The rootstock influences the development and primary metabolism of seedlings. P. aduncum showed greater initial compatibility among wild species, due to less impediment to carbohydrate flow. P. tuberculatum showed early incompatibility, as the grafts died. The seedlings grafted on P. hispidum and 'Kottanadan' had initial compatibility with an average of 78% survival and 60% budding, but they may have late incompatibility due to limitation of carbohydrate flow.

show abstract

“…Vegetable crops, particularly those from the Cucurbitacaeae and Solanaceae families, are extensively grafted for increased yield and enhanced stress tolerance ( Gaion et al, 2018 ). Facility cultivation producer would benefit from grafting to rootstocks that confer abiotic stress (i.e., cold) tolerance, which offer protection from soil-borne pathogens and maximize output by increasing yield ( Williams et al, 2021 ). The characterization and identification of resistance genes can amplify the contribution of a breeding program to improve rootstock resistance.…”

Section: Discussionmentioning

confidence: 99%

CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity

Wang

Zhou

et al. 2021

Front. Plant Sci.

View full text Add to dashboard Cite

Low-temperature stress is the main limiting factor of cucurbit crop cultivation as it affects crop yield and quality. The identification of genes involved in cold tolerance is a crucial aspect of pumpkin rootstock breeding. Here, we examined the function of a pumpkin Regulator of Chromosome Condensation 1 (CmRCC1) gene in the root development and cold stress responses of tobacco (Nicotiana benthamiana). CmRCC1 expression was differentially induced in pumpkin root, stem, and leaf under cold stress. Transient transformation showed that CmRCC1 is located in the nucleus. CmRCC1 overexpression in tobacco increased the gravitropic set-point angle in lateral roots, as well as root diameter and volume. The expression of auxin polar transport factors, PIN1 and PIN3, decreased and increased in CmRCC1-overexpressed plants, respectively. Yeast two-hybrid verification and luciferase complementation imaging assay showed that CmRCC1 interacts with CmLAZY1. Furthermore, the decreases in maximum quantum yield of PS II, the effective quantum yield of PS II, and electron transfer rate and the increases in quantum yield of nonregulated energy dissipation and malondialdehyde content were compromised in transgenic plants compared with wild-type plants under cold stress. The results suggest that CmRCC1 plays an important role in the regulation of root architecture and positively modulates cold tolerance.

show abstract

Getting to the root of grafting-induced traits

Cited by 25 publications

References 65 publications

Tomato Graft Union Failure Is Associated with Alterations in Tissue Development and the Onset of Cell Wall Defense Responses

Tomato Graft Union Failure Is Associated with Alterations in Tissue Development and the Onset of Cell Wall Defense Responses

Primary metabolism and initial development of grafted black pepper seedlings

CmRCC1 Gene From Pumpkin Confers Cold Tolerance in Tobacco by Modulating Root Architecture and Photosynthetic Activity

Contact Info

Product

Resources

About