Getting Across the Plasma Membrane and Beyond: Intracellular Uses of Colloidal Semiconductor Nanocrystals

Luccardini, Camilla; Yakovlev, A. V.; Gaillard, Stéphane; Hoff, Marcel van ‘t; Alberola, Alicia Piera; Mallet, Jean‐Maurice; Parak, Wolfgang J.; Feltz, Anne; Oheim, Martin

doi:10.1155/2007/68963

Cited by 22 publications

(20 citation statements)

References 78 publications

(109 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same limitations have already been discussed for quantum dots (for review, see Reference (113). In the field of DNA and plasmid delivery, it has previously been shown that lysosomotropic agents, such as chloroquine or sucrose (114), can improve DNA delivery and subsequent exogenous gene expression.…”

Section: Facilitated Deliverysupporting

confidence: 57%

Gold nanoparticles delivery in mammalian live cells: a critical review

et al. 2010

View full text Add to dashboard Cite

Functional nanomaterials have recently attracted strong interest from the biology community, not only as potential drug delivery vehicles or diagnostic tools, but also as optical nanomaterials. This is illustrated by the explosion of publications in the field with more than 2,000 publications in the last 2 years (4,000 papers since 2000; from ISI Web of Knowledge, ‘nanoparticle and cell’ hit). Such a publication boom in this novel interdisciplinary field has resulted in papers of unequal standard, partly because it is challenging to assemble the required expertise in chemistry, physics, and biology in a single team. As an extreme example, several papers published in physical chemistry journals claim intracellular delivery of nanoparticles, but show pictures of cells that are, to the expert biologist, evidently dead (and therefore permeable). To attain proper cellular applications using nanomaterials, it is critical not only to achieve efficient delivery in healthy cells, but also to control the intracellular availability and the fate of the nanomaterial. This is still an open challenge that will only be met by innovative delivery methods combined with rigorous and quantitative characterization of the uptake and the fate of the nanoparticles. This review mainly focuses on gold nanoparticles and discusses the various approaches to nanoparticle delivery, including surface chemical modifications and several methods used to facilitate cellular uptake and endosomal escape. We will also review the main detection methods and how their optimum use can inform about intracellular localization, efficiency of delivery, and integrity of the surface capping.

show abstract

Section: Facilitated Deliverysupporting

confidence: 57%

Gold nanoparticles delivery in mammalian live cells: a critical review

et al. 2010

View full text Add to dashboard Cite

show abstract

“…This method is non-invasive and can be used on an unlimited number of cells simultaneously. For experiments requiring QDs in the cytosol, including fluorescent labeling and potential therapeutic applications, this method can find immediate application 6,38,39. For intracellular targeting, pyrenebutyrate-mediated delivery can be coupled with developments in the functionalization of QDs to label specific cytosolic proteins 11,12,40-44.…”

mentioning

confidence: 99%

Pyrenebutyrate-Mediated Delivery of Quantum Dots across the Plasma Membrane of Living Cells

2008

View full text Add to dashboard Cite

Quantum dots have been delivered directly across the plasma membrane to the cytosol of living cells using a combination of a cationic peptide, polyarginine, and a hydrophobic counterion, pyrenebutyrate. Quantum dot delivery did not disrupt the plasma membrane and bypassed the barrier of endocytic vesicles. Cellular uptake was independent of temperature, but highly dependent on the surface charge of the quantum dot and the membrane potential of the cell, suggesting a direct translocation across the membrane. This method of delivery can find immediate application for quantum dots and may be broadly applicable to other nanoparticles.

show abstract

“…For many in vitro delivery applications, one would like NPs that directly reach the cytosol of the cells. 153 However, the cytosol is surrounded by the cellular membrane, which is a protein-enriched lipid double layer. Thus, there are only two principal ways to reach the cytosol.…”

Section: Nano Focusmentioning

confidence: 99%

The State of Nanoparticle-Based Nanoscience and Biotechnology: Progress, Promises, and Challenges

et al. 2012

Self Cite

View full text Add to dashboard Cite

Colloidal nanoparticles (NPs) have become versatile building blocks in a wide variety of fields. Here, we discuss the state-of-the-art, current hot topics, and future directions based on the following aspects: narrow size-distribution NPs can exhibit protein-like properties; monodispersity of NPs is not always required; assembled NPs can exhibit collective behavior; NPs can be assembled one by one; there is more to be connected with NPs; NPs can be designed to be smart; surface-modified NPs can directly reach the cytosols of living cells.

show abstract

Getting Across the Plasma Membrane and Beyond: Intracellular Uses of Colloidal Semiconductor Nanocrystals

Cited by 22 publications

References 78 publications

Gold nanoparticles delivery in mammalian live cells: a critical review

Gold nanoparticles delivery in mammalian live cells: a critical review

Pyrenebutyrate-Mediated Delivery of Quantum Dots across the Plasma Membrane of Living Cells

The State of Nanoparticle-Based Nanoscience and Biotechnology: Progress, Promises, and Challenges

Contact Info

Product

Resources

About