Getah Virus Infection among Racehorses, Japan, 2014

Nemoto, Manabu; Bannai, Hiroshi; Tsujimura, Koji; Kobayashi, Minoru; Kikuchi, Takuya; Yamanaka, Takashi; Kondo, Takashi

doi:10.3201/eid2105.141975

Cited by 62 publications

(75 citation statements)

References 12 publications

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“…Details of the horses from which the virus was isolated are given in Table 1. In our nucleic acid sequence analysis of the nsP1 and capsid genes, the sequences of the three isolates were identical to those of the RT-PCR products amplified from the clinical samples during the outbreak described in the previous study (12). In that study, the sequences were completely identical among the 10 specimens tested.…”

Section: Virus Isolation From Blood Samples Of Getah Virus-infected Hsupporting

confidence: 55%

“…It started in the middle of September and ended in late October. Among horses that were pyretic in this period, 33 were diagnosed with Getah virus infection through the detection of viral RNA by reverse transcription-PCR (RT-PCR) or by a significant increase in virus-neutralizing (VN) antibodies against Getah virus, or both (12). Because it had been believed that the vaccine was effectively protecting the horses at the training centers from the disease, the outbreak in 2014 was entirely unexpected to us.…”

Section: G Etah Virus Is Classified In the Genus Alphavirus In The Famentioning

confidence: 99%

“…Sera were collected from horses in the group of 33 that had been diagnosed as Getah virus infected during the outbreak at the Miho training center (12). Acute-phase sera were taken on the day the horses developed pyrexia, and convalescent-phase sera were taken at 20-to 30-day intervals.…”

Section: Methodsmentioning

confidence: 99%

“…To identify Getah virus-specific nucleotide sequences, the supernatants of specimens that showed cytopathic effect (CPE) were tested by RT-PCR using primer sets M 2 W-S and M 3 W-S for the nsP1 gene and SagW8 and SagW9 for the capsid gene (13). The RT-PCR products were sequenced as described previously (12,13).…”

Section: Methodsmentioning

confidence: 99%

“…In a previous study, we reported that an outbreak of Getah virus infection occurred in racehorses at the Miho training center in autumn 2014 (12). It started in the middle of September and ended in late October.…”

Section: G Etah Virus Is Classified In the Genus Alphavirus In The Famentioning

confidence: 99%

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Epizootiological Investigation of Getah Virus Infection among Racehorses in Japan in 2014

et al. 2015

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To clarify the factors causing an outbreak in 2014 of Getah virus infection among racehorses at the Miho training center, Japan, we isolated virus strains and performed an epizootiological investigation of affected horses and related horse populations. Three Getah virus isolates were recovered from clinical samples, and one of them (14-I-605) was used in a virus-neutralizing test. Of the affected horses (n ‫؍‬ 33), 20 (60.6%) were 2-year-olds. We investigated the histories of Getah virus vaccination of the affected horses and the whole population at the Miho training center. Among the 2-year-old population, the prevalence of the disease in horses that had been vaccinated once was 14.1%. This was significantly higher than that in horses that had been vaccinated twice or more (1.3%; P < 0.01). Among horses that had entered the training center from farms in Ibaraki Prefecture surrounding the training center and from neighboring Chiba Prefecture, the rate of seropositivity for Getah virus was 13.0% in September 2014 and 42.9% in October 2014; that in the corresponding periods in 2010 and 2013 was 0%. In conclusion, we identified two possible causes of the outbreak of Getah virus infection in the training center in 2014: (i) the existence of susceptible horses that had received only one dose of vaccination before the outbreak and (ii) increased risk of exposure to the virus because of epizootic Getah virus infection among horses on surrounding farms in Ibaraki and Chiba prefectures.

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Section: Virus Isolation From Blood Samples Of Getah Virus-infected Hsupporting

confidence: 55%

Section: G Etah Virus Is Classified In the Genus Alphavirus In The Famentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: G Etah Virus Is Classified In the Genus Alphavirus In The Famentioning

confidence: 99%

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Epizootiological Investigation of Getah Virus Infection among Racehorses in Japan in 2014

et al. 2015

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Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan

et al. 2016

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A Getah virus strain isolated during an outbreak in racehorses in Japan in 2014 (14-I-605) was compared with the vaccine strain isolated in 1978 (MI-110). A comparison of the genome sequences of these strains revealed seven amino acid substitutions in non-structural protein 3, and one or two substitutions in each of other non-structural proteins. In contrast, the structural proteins were highly conserved (99.8-99.9 % amino acid sequence identity). Horse antisera raised against the MI-110 strain showed similar virus-neutralization titers against both MI-110 and 14-I-605 strains (512 and 256, respectively). Therefore, antigenic mutation was probably not a direct cause of the outbreak that occurred in 2014.

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Getah virus epizootic among wild boars in Japan around 2012

et al. 2018

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In 2014, an outbreak of Getah virus (GETV) infection occurred in Japan in a horse population that was inoculated with a vaccine against GETV. In this study, we investigated the seroprevalence of GETV infection among wild boars in Japan. Interestingly, the highest rate of anti-GETV-positive wild boars was observed in 2013, which gradually decreased during 2014-2016. The results suggested that GETV spread among wild boars around 2012, resulting in the 2014 outbreak.

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Getah Virus Infection among Racehorses, Japan, 2014

Cited by 62 publications

References 12 publications

Epizootiological Investigation of Getah Virus Infection among Racehorses in Japan in 2014

Epizootiological Investigation of Getah Virus Infection among Racehorses in Japan in 2014

Genomic, pathogenic, and antigenic comparisons of Getah virus strains isolated in 1978 and 2014 in Japan

Getah virus epizootic among wild boars in Japan around 2012

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