Gestrinone inhibits growth of human uterine leiomyoma may relate to activity regulation of ERα, Src and P38 MAPK

Ginsenoside Rh2 suppresses growth of uterine leiomyoma in vitro and in vivo and may regulate ERα/c-Src/p38 MAPK activity

“…Cells were treated for 72 h with DMSO or graded concentrations (0.3, 3.0, and 10 µM) of Rh2 for 72 h and collected. ELISA analyses were performed according to manufacturer's instructions and our previous report (Zhu et al, 2012). The presented values are the average ratio of p38/GAPDH, phosphorylatedp38 (P-p38)/GAPDH, and P38/P-p38 in three different samples.…”

“…Briefly, adherent cells were cultured and fixed in 96-well Nunc plates. Experiments were performed according to the manufacturer's instructions and our previous report (Zhu et al, 2010(Zhu et al, , 2012. At least 1000 cells were analysed in each sample.…”

“…Cells were cultured in 96-well plates and treated with DMSO or graded concentrations of Rh2 (0.01, 0.1, 1.0, 10, 50, or 100 µmol/L) for 24, 48, and 72 h, according to our previous report (Zhu et al, 2010(Zhu et al, , 2012. Using a spectrophotometric plate reader (Bio-Tek ELX-800, Winooski, VT, USA), the absorbance (OD) at 450 nm was recorded to determine cell viability.…”

“…UL cells were cultured as we described previously (Zhu et al, 2010(Zhu et al, , 2012. Briefly, UL tissue samples were digested at 37°C in pre-warmed 0.25% trypsin-EDTA and HBSS containing 2.0% type II collagenase (wt/vol) until the tissues disintegrated.…”

“…Here, we investigated the impact of Rh2 on UL growth in vitro and in vivo. Because activation of c-Src and p38 MAPK were reported to contribute to the growth of ULs (Zhu et al, 2007(Zhu et al, , 2010(Zhu et al, , 2012, we further investigated the effect of Rh2 on several signalling molecules, including oestrogen receptor alpha (ERα), c-Src, and p38 MAPK.…”