Gestational Exposure to Bisphenol A Affects the Function and Proteome Profile of F1 Spermatozoa in Adult Mice

Rahman, Saidur; Kwon, Woo-Sung; Karmakar, Polash Chandra; Yoon, Se-Jin; Ryu, Buom‐Yong; Pang, Myung-Geol

doi:10.1289/ehp378

Cited by 120 publications

(125 citation statements)

References 51 publications

(74 reference statements)

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“…Samples were centrifuged at 700 × g for 15 min with isotonic 45% Percoll in PBS [4, 17, 19]. After rehydrating with 250 μg solubilized protein samples for 12 h at 4 °C, 24-cm-long NL Immobiline DryStrips (pH 3–11; Amersham, Piscataway, NJ, USA) were focused for first-dimension electrophoresis using an IPGphor isoelectric focusing system.…”

Section: Methodsmentioning

confidence: 99%

Proteomic identification of cryostress in epididymal spermatozoa

Yoon

Rahman

Kwon

et al. 2016

J Animal Sci Biotechnol

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Background: Cryopreservation of epididymal spermatozoa is important in cases in which it is not possible to collect semen using normal methods, as the sudden death of an animal or a catastrophic injury. However, the freezing and thawing processes cause stress to spermatozoa, including cold shock, osmotic damage, and ice crystal formation, thereby reducing sperm quality. We assessed the motility (%), motion kinematics, capacitation status, and viability of spermatozoa using computer-assisted sperm analysis and Hoechst 33258/chlortetracycline fluorescence staining. Moreover, we identified proteins associated with cryostress using a proteomic approach and performed western blotting to validate two-dimensional electrophoresis (2-DE) results using two commercial antibodies. Results: Cryopreservation reduced viability (%), motility (%), straight-line velocity (VSL), average path velocity (VAP), amplitude of lateral head displacement (ALH), and capacitated spermatozoa, whereas straightness (STR) and the acrosome reaction increased after cryopreservation (P < 0.05). Nine proteins were differentially expressed (two proteins decreased and seven increased) (>3 fold, P < 0.05) before and after cryopreservation. The proteins differentially expressed following cryopreservation are putatively related to several signaling pathways, including the ephrinR-actin pathway, the ROS metabolism pathway, actin cytoskeleton assembly, actin cytoskeleton regulation, and the guanylate cyclase pathway.

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Section: Methodsmentioning

confidence: 99%

Proteomic identification of cryostress in epididymal spermatozoa

Yoon

Rahman

Kwon

et al. 2016

J Animal Sci Biotechnol

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“…Versatile role of protein acetylation is obvious, including aforementioned residual histones as well as protein kinases. Taken together, the impact of endocrine disruptors on histone PTMs [23,24] as well as sperm phosphorylation [25][26][27] has been described, and, therefore, the modifications of proteins (protein PTMs) become the likely manner in which disruptors (EDs) work in their real doses.…”

Section: Endocrine Disruptors Induce Non-genomic Inheritance Through mentioning

confidence: 99%

“…The comparable effect of EDs is known during spermatogenesis: BPA is capable of affecting meiotic division and chromosome segregation, increasing the incidence of aneuploidy-derived disorders [73]. In addition, the molecular mechanism of BPA consists in impacting several signal pathways and results in the change of protein kinase A activity and protein tyrosine phosphorylation, ATP generation, and oxidative stress-related enzymes (i.e., peroxiredoxin-5, glutathione peroxidase 4, succinate dehydrogenase), crucial for sperm motility and ability of oocyte fertilization [26,27,74]. Dose-response association of BPA and motility parameters of human sperm has been observed [75].…”

Section: Molecular Mechanism Of Eds In Extremely Low Dosesmentioning

confidence: 99%

Endocrine Disruptors: Very Low Doses with Genuinely High Impacts on Male Reproduction

Ješeta¹,

Nevoral²

2020

Male Reproductive Health

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Endocrine disruptors (EDs) are chemical substances that affect physiological processes in the body via hormonal regulation. They are often detected in food, plastic water bottles, cosmetics, and many other daily need items. Thereafter, EDs are detected in many bodily fluids, pointing out the real exposure to even very low doses. Permanent and long-term utilization of EDs has harmful effects on male reproductive health mainly due to interference with sex hormone synthesis and mechanism of action. However, with decreasing dosage of EDs, the possibilities of unpredictable modes of action arise. In addition to various molecular actions of individual EDs, the interference of individual ones represents another dimension of the ED issue. This review provides an overview of the EDs and their possible impact on reproductive health in males, with focus on sperm quality with the mighty potential of epigenetic transmission to further generations. The "posttranslational" effect of EDs in really low doses in real exposure routes is stigmatized in this review, being strongly considered as creeping molecular action of individual EDs as well as amplifications of their copresence in the environment.

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“…Recently, attempts have been made to develop reproductive biomarkers using comprehensive proteomic approaches (Kwon et al ., ,d). To date, two‐dimensional electrophoresis (2‐DE; iTRAQ, DIGE, etc) and mass spectrometry have been used to identify protein markers in various comparative studies [normal vs. malformed, immature vs. mature, capacitated vs. non‐capacitated (NC), low fertility vs. high fertility, cryopreserved vs. fresh spermatozoa, and good freezers vs. bad freezers] (Jagan Mohanarao & Atreja, ; Park et al ., ; Xu et al ., ; Vilagran et al ., 2013; Kwon et al ., 2014b; Wang et al ., ; Vilagran et al ., ; Rahman et al ., ,b). However, most of these studies were species‐ or breed‐specific and were conducted in an in vitro setting.…”

Section: Introductionmentioning

confidence: 99%

Comparison of markers predicting litter size in different pig breeds

et al. 2017

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To overcome the limitations of conventional analysis of male fertility in animals and humans, proteomic studies have been performed to develop fertility-related biomarkers for prognosis and diagnosis of male fertility. However, the studies were focused on specific species or breeds. Therefore, a study is required to validate whether fertility-related markers would apply to other breeds in pigs. In this study, previously developed fertility-related biomarkers from Landrace were validated to use for prognosis of male fertility in commercially available breeds. Expression level of eight biomarkers in non-capacitated and capacitated (C) spermatozoa from Yorkshire and Duroc boars was analyzed. And then, to explore the validity of these markers for prognosis of male fertility, i.e. litter size, artificial insemination was performed. Among them, RAB2A (NC) and UQCRC1 (NC) turned out to be highest efficient markers for Yorkshire. RAB2A (C) was most efficient marker for Duroc. Average litter size has increased as much as 1.41 live born after prediction using eight fertility-related biomarkers in Yorkshire. In addition, average 2.52 litter size was increased after prediction using eight fertility-related biomarkers in Duroc. Average litter sizes were especially highly increased after prediction of fertility using RAB2A (NC) in Yorkshire (1.57 piglets) and TPI (NC) in Duroc (3.14 piglets), respectively. As a result, all biomarkers were significantly correlated with litter size. However, overall accuracy to predict litter size in three breeds was different in response with each marker. Average litter size after artificial insemination was also significantly affected by marker selection. Therefore, this study suggests that developed fertility-related markers may be used for prognosis and diagnosis of male fertility irrespective of breed. However, selection of efficient markers for breeds should be considered to obtain more accurate and efficient outcomes.

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Gestational Exposure to Bisphenol A Affects the Function and Proteome Profile of F1 Spermatozoa in Adult Mice

Cited by 120 publications

References 51 publications

Proteomic identification of cryostress in epididymal spermatozoa

Proteomic identification of cryostress in epididymal spermatozoa

Endocrine Disruptors: Very Low Doses with Genuinely High Impacts on Male Reproduction

Comparison of markers predicting litter size in different pig breeds

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