Geroprotective effects of ×Sorbaronia mitschurinii fruit extract on Drosophila melanogaster

Abstract

BACKGROUND: ×Sorbaronia mitschurinii (also known as Aronia mitschurinii) is an intergeneric hybrid of×Sorbaronia fallax and Aronia melanocarpa. ×S. mitschurinii berry is a rich source of phytochemicals such as flavonoids and anthocyanins, which have a broad range of health benefits and a great geroprotective potential. OBJECTIVES: The goal of the present study was to investigate the geroprotective effects of Sorbaronia berry ethanolic extract (SBE) in Drosophila melanogaster and whether these effects depend on the concentration of SBE, duration of treatment, age, and sex. METHODS: SBE was used to supplement a diet of Drosophila imagoes throughout life, during 2 weeks after the imago hatching, and from 4 to 6 weeks of age. The relationship between the SBE effects on lifespan, stress resistance and the expression of stress response genes were examined. RESULTS: SBE treatment at 1-2 and 4-6 weeks of life increased the lifespan, while treatment throughout life reduced lifespan. SBE treatment increased Drosophila resistance to oxidative stress and starvation, but not to hyperthermia. A statistically significant effect of SBE treatment on the expression level of per, keap1, hif1, hsp27, hsp68, hsp83, and sirt1 genes in flies of both sexes and on sod1 expression only in female flies was demonstrated. CONCLUSIONS: The observed relationship between SBE concentration and lifespan effects suggests that the life-extending effect of SBE may be associated with the mechanism of hormesis.

Antioxidant Properties and Geroprotective Potential of Wheat Bran Extracts with Increased Content of Anthocyanins

“…At the same time, it is obvious that the samples from anthocyanin-free and anthocyanin-containing wheats do not contain sufficient concentrations of lipids and carbohydrates that could improve the adaptation of D. melanogaster to conditions of starvation. A previously studied ethanol extract derived from chokeberry (×Sorbaronia mitschurinii) also did not demonstrate a statistically significant impact on resistance to oxidative stress and hyperthermia, but it increased the survival time of D. melanogaster females under conditions of starvation [25].…”

“…For example, anthocyanin extract from cranberry (Vaccinium macrocarpon L.) was shown to increase the average lifespan of male flies by 10% and also reduce the mortality rate caused by treatment with hydrogen peroxide (H 2 O 2 ) [24]. In research by our group, it was previously established that anthocyanin extracts from the fruits of chokeberry (×Sorbaronia mitschurinii) [25], honeysuckle (Lonicera caerulea L.) [26], and rowan (Sorbus aucuparia L.) [27] exhibit antioxidant activity and contribute to an increase in the lifespan of D. melanogaster. This data suggests that anthocyanins possess geroprotective potential.…”

Chokeberry (×sorbaronia mitschurinii ) extract delays age disturbance of Drosophila melanogaster intestinal barrier

“…В предварительном исследовании нами обнаружено, что применение этанольного экстракта плодов черноплод-ной рябины в концентрациях 0.01, 0.1, 1, 2.5, 5 и 10 мг/мл на протяжении всей жизни D. melanogaster не оказывало отрицательного воздействия на проницаемость кишечного барьера в возрасте двух, шести и восьми недель [16].…”

“…Экспериментально было обнаружено, что кратковременная обработка имаго D. melanogaster в возрасте с четырех по шестую неделю этанольным экстрактом плодов ×Sorbaronia mitschurinii (ABE) вызывает увеличение медианной (до 5 %) и максимальной (до 9 %) продолжительности жизни. При этом обработка мух экстрактом в первые две недели жизни или на протяжении всей жизни оказывала негативный эффект на медианную и максимальную продолжительность жизни (снижение до 9 %) [16].…”

Exploring the neuroprotective effects of chokeberry (×Sorbaronia mitschurinii) extract on Drosophila melanogaster model of Alzheimer’s disease

“…Опубликованные Afzal et al [24] и Suresh et al [25] систематические обзоры доклинических данных показали, что антоцианы обладают потенциальным терапевтическим эффектом при болезни Альцгеймера, снижая окислительный стресс, воспаление и накопление бета-амилоида. Также установлено, что экстракт черноплодной рябины увеличивает продолжительность жизни и снижает возрастной окислительный стресс у Drosophila melanogaster [13,26].…”