Germline transmission of MSTN knockout cattle via CRISPR-Cas9

Gim, Gyeong-Min; Uhm, Kyeong-Hyun; Kwon, Dongil; Kim, Minji; Jung, Dae-Jin; Kim, Dae-Hyun; Yi, Junkoo; Ha, Jae Jung; Yum, Soo‐Young; Son, Woo-Jae; Lee, Ji‐Hyun; Park, Jihyun; Song, Kil-Young; Lee, Won-Wu; Jang, Goo

doi:10.1016/j.theriogenology.2022.08.021

Cited by 9 publications

(9 citation statements)

References 12 publications

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“…Researchers have successfully used CRISPR/Cas9 to target genes related to adipose tissue development in cattle. For instance, they can disrupt or modify transcription factors involved in adipogenesis to investigate their roles (de Camargo & Pereira, 2022; Ge et al., 2021; Gim et al., 2021; Gim, Kwon, et al., 2022; Gim, Uhm, et al., 2022; Singh & Ali, 2021; Tan et al., 2013).…”

Section: Functional Genomics Methodsmentioning

confidence: 99%

A review of the role of transcription factors in regulating adipogenesis and lipogenesis in beef cattle

Abebe,

Wang,

et al. 2023

J Animal Breeding Genetics

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In the past few decades, genomic selection and other refined strategies have been used to increase the growth rate and lean meat production of beef cattle. Nevertheless, the fast growth rates of cattle breeds are often accompanied by a reduction in intramuscular fat (IMF) deposition, impairing meat quality. Transcription factors play vital roles in regulating adipogenesis and lipogenesis in beef cattle. Meanwhile, understanding the role of transcription factors in regulating adipogenesis and lipogenesis in beef cattle has gained significant attention to increase IMF deposition and meat quality. Therefore, the aim of this paper was to provide a comprehensive summary and valuable insight into the complex role of transcription factors in adipogenesis and lipogenesis in beef cattle. This review summarizes the contemporary studies in transcription factors in adipogenesis and lipogenesis, genome‐wide analysis of transcription factors, epigenetic regulation of transcription factors, nutritional regulation of transcription factors, metabolic signalling pathways, functional genomics methods, transcriptomic profiling of adipose tissues, transcription factors and meat quality and comparative genomics with other livestock species. In conclusion, transcription factors play a crucial role in promoting adipocyte development and fatty acid biosynthesis in beef cattle. They control adipose tissue formation and metabolism, thereby improving meat quality and maintaining metabolic balance. Understanding the processes by which these transcription factors regulate adipose tissue deposition and lipid metabolism will simplify the development of marbling or IMF composition in beef cattle.

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Section: Functional Genomics Methodsmentioning

confidence: 99%

A review of the role of transcription factors in regulating adipogenesis and lipogenesis in beef cattle

Abebe,

Wang,

et al. 2023

J Animal Breeding Genetics

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show abstract

“…One of the most recent studies on the generation of GEC using CRISPR/Cas9 involved the targeted editing of the MSTN gene, which doubled the muscle mass [ 62 ]. It has been confirmed that the cattle can transmit the MSTN mutation to the next generation, indicating stable germline transmission of the trait [ 63 ].…”

Section: Crispr/cas9mentioning

confidence: 99%

Outlook on genome editing application to cattle

Gim,

Jang

2024

J Vet Sci

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In livestock industry, there is growing interest in methods to increase the production efficiency of livestock to address food shortages, given the increasing global population. With the advancements in gene engineering technology, it is a valuable tool and has been intensively utilized in research specifically focused on human disease. In historically, this technology has been used with livestock to create human disease models or to produce recombinant proteins from their byproducts. However, in recent years, utilizing gene editing technology, cattle with identified genes related to productivity can be edited, thereby enhancing productivity in response to climate change or specific disease instead of producing recombinant proteins. Furthermore, with the advancement in the efficiency of gene editing, it has become possible to edit multiple genes simultaneously. This cattle breed improvement has been achieved by discovering the genes through the comprehensive analysis of the entire genome of cattle. The cattle industry has been able to address gene bottlenecks that were previously impossible through conventional breeding systems. This review concludes that gene editing is necessary to expand the cattle industry, improving productivity in the future. Additionally, the enhancement of cattle through gene editing is expected to contribute to addressing environmental challenges associated with the cattle industry. Further research and development in gene editing, coupled with genomic analysis technologies, will significantly contribute to solving issues that conventional breeding systems have not been able to address.

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“…2B ) ( 9 , 50 , 51 ). The electroporation has enabled the production of gene-engineered livestock animals through a simpler method as opposed to the previously laborious MI ( 9 , 52 ). Therefore, various types of gene-engineered livestock animals have been created using gene editing tools, resulting in enhanced productivity, disease resistance, and advancements in biomedicine.…”

Section: In Vitro Animal Production Technologymentioning

confidence: 99%

Current status and future of gene engineering in livestock

Kwon,

Gim,

Yum

et al. 2024

BMB Rep

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The application of gene engineering in livestock is necessary for various reasons, such as increasing productivity and producing disease resistance and biomedicine models. Overall, gene engineering provides benefits to the agricultural and research aspects, and humans. In particular, productivity can be increased by producing livestock with enhanced growth and improved feed conversion efficiency. In addition, the application of the disease resistance models prevents the spread of infectious diseases, which reduces the need for treatment, such as the use of antibiotics; consequently, it promotes the overall health of the herd and reduces unexpected economic losses. The application of biomedicine could be a valuable tool for understanding specific livestock diseases and improving human welfare through the development and testing of new vaccines, research on human physiology, such as human metabolism or immune response, and research and development of xenotransplantation models. Gene engineering technology has been evolving, from random, time-consuming, and laborious methods to specific, time-saving, convenient, and stable methods. This paper reviews the overall trend of genetic engineering technologies development and their application for efficient production of genetically engineered livestock, and provides examples of technologies approved by the United States (US) Food and Drug Administration (FDA) for application in humans.

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Germline transmission of MSTN knockout cattle via CRISPR-Cas9

Cited by 9 publications

References 12 publications

A review of the role of transcription factors in regulating adipogenesis and lipogenesis in beef cattle

A review of the role of transcription factors in regulating adipogenesis and lipogenesis in beef cattle

Outlook on genome editing application to cattle

Current status and future of gene engineering in livestock

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