Germination of Spores of Cantharellus Cibarius

Fries, Nils

doi:10.1080/00275514.1979.12021003

Cited by 17 publications

(7 citation statements)

References 7 publications

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“…The first report of pure chanterelle culture establishment described yellow colonies (representative strain 740b) obtained using a spore isolation technique; these colonies had a similar fragrance to the fruiting bodies (Fries, 1979). Strain 740b was identified as true C. cibarius using a DNA hybridization technique (Straatsma, Konings, & van Griensven, 1985).…”

Section: Foundations Of Chanterelle Cultivationmentioning

confidence: 99%

“…Cantharellus anzutake rarely produces pure cultures from basidiomata (Ogawa et al, 2019a); even C. anzutake cultured from basidiomata under laboratory conditions (Ogawa et al, 2019b) exhibit bacterial and fungal contamination during tissue isolation procedures (unpublished data), as has been reported in C. cibarius (Pilz et al, 2003). However, multispore isolation techniques developed for C. cibarius by Fries (1979) have shown that young, fresh basidioma can produce pure cultures. In C. anzutake, pure cultures have been successfully obtained from field-sampled ectomycorrhizal root tips (Ogawa et al, 2019a).…”

Section: Chanterelle Cultivation Bottleneckmentioning

confidence: 99%

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Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies

Yamada

2022

Mycoscience

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Edible ectomycorrhizal mushrooms such as truffles (Tuber), chanterelles (Cantharellus cibarius Fr. and related species), porcinis (Boletus edulis Bull. and related species), saffron milk caps (Lactarius deliciosus [L.] Gray and related species), and matsutakes (Tricholoma matsutake [S. Ito & S. Imai] Singer and related species) have high economic value worldwide (Zambonelli & Bonito, 2012; Pérez-Moreno, Guerin-Laguette, Arzú, & Yu, 2020), with annual global sales estimated at several billion dollars. These mushrooms are harvested in forests or plantations for both domestic and export markets (Arora, 2008;Tsing, 2015, Pérez-Moreno et al., 2021. In Japan, imported matsutake mushrooms from countries such as China, USA, Canada, Morocco, Turkey, Mexico, and South Korea are valued at 80 to 100 million dollars annually (Aoki et al., 2022), which is equivalent to domestic T. matsutake production within Japan, mainly in Nagano and Iwate Prefectures (Yamanaka, . During the past 20 y, truffle imports to Japan have increased such that their economic value in Japan now equivalent to around 10% that of the matsutake mushroom import value (MAFF; https://www.maff.go.jp/e/index.html).To meet this increasing market demand, truffles have been harvested in tree plantations (Zambonelli, Iotti, & Murat, 2016). However, other edible mushrooms are largely harvested in forests because non-host and tree plantation cultivation techniques have not yet been established. Therefore, these mushrooms must be conserved in natural forests through controlled harvests or forest management to sustain moderate harvest levels for longer (decade-scale) periods (

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Section: Foundations Of Chanterelle Cultivationmentioning

confidence: 99%

Section: Chanterelle Cultivation Bottleneckmentioning

confidence: 99%

Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations in vitro and efficient production of fruiting bodies

Yamada

2022

Mycoscience

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“…The fruit bodies of C. cibarius and some re-E. Danell (Danell et al 1993), and this, together with the slow growth, has been the main reason for the lack of success in isolating sterile mycelium from fruit bodies (Danielson 1984;Froidevaux 1975;It~ivaara and Willberg 1988;Modess 1941;Schouten and Waandrager 1979). Fries (1979) did the first successful germination of spores, since repeated by others (Danell and Fries 1990;It~ivaara and Willberg 1988;Straatsma et al 1985). Straatsma et al (1985) conducted the first isolation of mycelium from fruit bodies, but their technique has been successfully used only by Moore et al (1989) and Danell and Fries (1990).…”

Section: Introductionmentioning

confidence: 95%

Formation and growth of the ectomycorrhiza of Cantharellus cibarius

Danell

1994

Mycorrhiza

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New data on the physiology of Cantharellus cibarius mycorrhiza formation has resulted in a new aseptic routine method for in vitro formation. The advances are short formation time, healthy plants and reliable colonization. A high glucose demand and a good gas exchange with additional carbon dioxide are important factors in the mycorrhiza formation. Mycorrhiza was observed after 8 weeks, but strong colonization occurred after 10-12 weeks, when mycorrhiza was established to the depth of 5 cm. A C. cibarius strain connected to Picea abies in nature successfully colonized Pinus sylvestris in vitro, but not Betula pendula. Mycorrhizal plants have been successfully transferred to unsterile environments in greenhouses. The mycorrhizae continued to colonize new roots and the unsterile peat soil for 10 months. However, C. cibarius mycorrhiza is highly sensitive to flooding. With PCR and RFLP, fruit bodies, isolated mycelia and artificially formed mycorrhizae have been compared to prove that C. cibarius was used. Climatic changes did not induce primordia formation, but factors behind fruit body formation are discussed.

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“…It is generally accepted that it is difficult to establish pure cultures from basidiomata tissues of C. cibarius due to considerable contamination by bacteria and fungi (Modess, 1941;Schouten & Waandrager, 1979;Danell & Fries, 1990;Danell, 1994). However, Fries (1979) reported validly characterized pure cultures of C. cibarius. Strain 740b established by multispore isolation on nutrient agar medium showed a yellowish colony color and a specific fragrance similar to that of the fruit body of C. cibarius (Fries, 1979;Straatsma, Konings, & van Griensven, 1985).…”

Section: Introductionmentioning

confidence: 99%

“…However, Fries (1979) reported validly characterized pure cultures of C. cibarius. Strain 740b established by multispore isolation on nutrient agar medium showed a yellowish colony color and a specific fragrance similar to that of the fruit body of C. cibarius (Fries, 1979;Straatsma, Konings, & van Griensven, 1985). This strain was subsequently identified as a true C. cibarius culture by a DNA hybridization technique (Straatsma, Konings, & van Griensven, 1985).…”

Section: Introductionmentioning

confidence: 99%

Efficient establishment of pure cultures of yellow chanterelle Cantharellus anzutake from ectomycorrhizal root tips, and morphological characteristics of ectomycorrhizae and cultured mycelium

et al. 2019

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Species of fleshy yellow Cantharellus are known as chanterelles, which are among the most popular wild edible mycorrhizal mushrooms in the world. However, pure culture isolates of Cantharellus are rare. We report an efficient isolation technique of the Japanese golden chanterelle, Cantharellus anzutake, from its ectomycorrhizal root tips. Field-sampled fresh ectomycorrhizal root tips of C. anzutake on various hosts such as pines, spruce, and oaks were vortexed with 0.005% Tween 80 solution, surface sterilized with 1% calcium hypochlorite solution, rinsed with sterilized distilled water, and placed on modified Norkrans' C (MNC) agar plate medium. Most ectomycorrhizal root tips of C. anzutake produced yellowish mycelial colonies within a few months. In contrast, tissue isolation from basidiomata provided limited cultures of C. anzutake but much contamination of bacteria and molds, even on media that contained antibiotics. The established C. anzutake cultures had clamp connections on the hyphae and contained intracellular oily droplets. These cultured isolates were identified as C. anzutake by sequence analysis of the rRNA internal transcribed spacer (ITS) region and translation elongation factor EF1-alpha (tef-1) genes.

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Germination of Spores of Cantharellus Cibarius

Cited by 17 publications

References 7 publications

Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations <i>in vitro</i> and efficient production of fruiting bodies

Cultivation studies of edible ectomycorrhizal mushrooms: successful establishment of ectomycorrhizal associations <i>in vitro</i> and efficient production of fruiting bodies

Formation and growth of the ectomycorrhiza of Cantharellus cibarius

Efficient establishment of pure cultures of yellow chanterelle Cantharellus anzutake from ectomycorrhizal root tips, and morphological characteristics of ectomycorrhizae and cultured mycelium

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