Germination of Brassica pollen and expression of incompatibility in vitro

Ferrari, Thomas E.; Wallace, Don

doi:10.1007/bf00132915

Cited by 37 publications

(18 citation statements)

References 14 publications

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“…Regarding the effectiveness of the heat-treatment in overcoming inhibition by the diffusate on self-pollen, there is a similar report for Brassica (Ferrari and Wallace 1975) also obtained by testing self-pistil leachate in culture. The involvement of heat-labile substances in the incompatibility reaction, which has been observed here, agrees in turn with the well-known fact that temperature significantly affects plant incompatibility (see reviews by De Nettancourt 1977;Shivanna and Johri 1985).…”

Section: Discussionsupporting

confidence: 51%

“…The following examples have been reported by HeslopHarrison (1983), who stressed, however, the difficulties in obtaining clear-cut effects in vitro: Petunia inflata and P. hybrida (Brewbaker and Majumder 1961 ;Sharma and Shivanna 1982), and Lilium longiflorum (Dickinson et al 1982). To this Offprint requests to: A. Speranza list, Brassica oleracea (Ferrari and Wallace 1975), Prunus avium (Williams et al 1982) and Nicotiana alata (Sharma and Shivanna 1985) should be added.…”

Section: Introductionmentioning

confidence: 99%

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In vitro test of self-incompatibility in Malus domestica

Speranza

Calzoni

1988

Sexual Plant Reprod

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An in vitro assay in which self-incompatible pollen of Malus domestica is selectively inhibited is described. This assay involves heat-labile substances diffusing from the stylar tissues -in particular, glycoproteins found in the protein extract of styles. In the presence of the self-style extract, a dramatic decrease in total protein concentration in the culture medium was revealed at 30-min germination. Pretreatment of the self-pollen with 100 mM glucose prevented this drop in protein level; moreover, tube growth was entirely restored. A possible explanation in terms of protein-carbohydrate complementation is suggested.

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Section: Discussionsupporting

confidence: 51%

Section: Introductionmentioning

confidence: 99%

In vitro test of self-incompatibility in Malus domestica

Speranza

Calzoni

1988

Sexual Plant Reprod

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“…Recognition must therefore occur within minutes of pollination (Ferrari and Wallace, 1975). The events immediately following the first contact between the pollen grain and the stigma have thus drawn much interest.…”

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confidence: 99%

Pollen-Stigma Adhesion in Kale Is Not Dependent on the Self-(In)Compatibility Genotype

1997

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The adhesion of pollen on the stigmas of flowering plants is a critical step for the success of reproduction in angiosperms, long considered to present some specificity in terms of self-incompatibility. We carried out quantitative measurements of the pollen-stigma adhesion (expressed in Newtons) in kale (Brassica oleracea), using the flotation force of Archimedes exerted by dense sucrose solutions (50%, w/v) to release pollen grains fixed on the surface of stigmas. We demonstrate that pollen adhesion varies with the genotypes of the plants used as partners, but increases with time in all cases for about 30 to 60 min after pollination. There is no correlation with the self- or cross-status of the pollinations, nor with the self-compatible or -incompatible genotypes of the parents. Only late events of pollination, after the germination or arrest of the pollen tube, depend on compatibility type. Biochemical and physiological dissection of pollen-stigma adhesion points to major components of this interaction: among male components, the pollen coating, eliminated by delipidation (or modified by mutation in the case of the cer mutants of the related species Arabidopsis thaliana), plays a major role in adhesion; the genetic background of the pollen parent is also of some importance. On the female side, the developmental stage of the stigma and the protein constituents of the stigmatic pellicle are critical for pollen capture. The SLG and SLR1 proteins are not involved in the initial stages of pollen adhesion on the stigma but one or both may be involved in the later stages.

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“…Suspension media composed of varying amounts of polyethylene glycol, calcium pectate, sodium caseinate or sodium alginate all reduced pollen viability, particularly after 1 h of drying. Ferrari & Wallace (1975) reported that germination of cabbage pollen was dependent on the presence of polyethylene glycol (M. Wt 20,(00) but we have been unable to repeat these findings with the pollens we have tested. However, suspension media composed ofCMC plus calcium nitrate and boric acid, partially maintained kiwifruit pollen viability after drying ( Fig.…”

Section: Discussionmentioning

confidence: 69%

I. Development of suspension media

Hopping¹,

Jerram²

1980

New Zealand Journal of Agricultural Research

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A number of aqueous solutions were evaluated as possible pollen carriers for the spray pollination of fruit trees. The viability of apple, kiwifruit, oil palm, and plum pollen was markedly reduced after pollen was suspended in water. This loss of viability could be partly overcome by slow hydration of the pollen in moist air before its suspension. The viability of hydrated oil palm and kiwifruit pollen after suspension in calcium nitrate solution of low concentration with or without boric acid, was similar to that of hydrated pollen germinated directly. Similar results were obtained with apple and plum pollens, provided that pentaerythritol was added to the suspension medium. However, the viability of apple , kiwifruit, and plum pollen that had been suspended in these media was substantially reduced during drying on a simulated stigma surface. For kiwifruit, the loss of pollen viability after drying could be partially overcome by the further addition of sodium carboxymethyl cellulose, with or without sodium alginate, to the suspension medium.

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Germination of Brassica pollen and expression of incompatibility in vitro

Cited by 37 publications

References 14 publications

In vitro test of self-incompatibility in Malus domestica

In vitro test of self-incompatibility in Malus domestica

Pollen-Stigma Adhesion in Kale Is Not Dependent on the Self-(In)Compatibility Genotype

I. Development of suspension media

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