Germination Capability of Wheat Seeds in Correlation with Delayed Luminescence Intensity

Garofalo, Rebeca T.; Moraes, Thiago Alexandre; Gallep, Cristiano M.

doi:10.1364/laop.2010.mb03

Cited by 2 publications

(2 citation statements)

References 6 publications

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“…The DL detection has better signal and sensitivity than spontaneous photon emission (SPE), make it an ideal choice for a reliable and inexpensive optical biopsy technique. The application of DL detection technologies had been well studied in many fields, including food safety inspection 8 , natural organic matter detection 9 , water pollution detection 10 , physiological characteristics of plants detection 11 , and seed quality measurement and analysis 12 , 13 , Chinese herbal medicine identification 14 , 15 , clinical application, human health 16 – 18 and so on. At the same time, some theoretical progress has been made based on these technical application.…”

Section: Introductionmentioning

confidence: 99%

Relationship between delayed luminescence emission and mitochondrial status in Saccharomyces cerevisiae

Tian¹,

Li²,

Liu³

et al. 2022

Sci Rep

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Delayed luminescence (DL) is gradually used in various detection of biological systems as a rapid detection technique, however, its biological mechanism was still not clear. In this study, a new model of DL detection system for liquid biological samples is established to investigate the DL emission of Saccharomyces cerevisiae cells cultured in different glucose concentrations. We analyzed the relationship between the DL emission and cell growth, cell vitality, mitochondrial morphology, mitochondrial DNA (mtDNA) copy number, adenosine triphosphate (ATP), oxygen consumption rate (OCR), as well as mitochondria membrane potential (MMP) in S. cerevisiae cells cultured with 0.01, 0.05, 0.15, 3, 10 and 20 g/L glucose respectively. It was found that the DL emission had strong correlation with mitochondrial morphology, OCR, and MMP. The results suggested that DL is an indicator of mitochondria status under different glucose supply conditions, and may be an effective method to detect mitochondrial metabolism related disorders.

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Section: Introductionmentioning

confidence: 99%

Relationship between delayed luminescence emission and mitochondrial status in Saccharomyces cerevisiae

Tian¹,

Li²,

Liu³

et al. 2022

Sci Rep

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“…Estimations of DL from various water‐rich vegetables and fruits showed relationship of DL to development and maturity (ripeness) . Another DL application is in the field of germination capacity of seeds . DL measurement may be able to correlate to the functional state of biological systems in living materials .…”

Section: Introductionmentioning

confidence: 99%

Delayed luminescence: an experimental protocol for Chinese herbal medicines

Sun

Wijk

et al. 2016

Luminescence

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In Chinese medicine, raw herbal materials are used in processed and unprocessed forms aiming to meet the different requirements of clinical practice. To assure the chemical quality and therapeutic properties of the herbs, fast and integrated systematic assays are required. So far, such assays have not been established. Delayed luminescence (DL) refers to a decaying long-term ultraweak photon emission after exposure to light. Its decay kinetics under certain conditions may be a sensitive indicator reflecting the internal structural and chemical/physiological state of a biological system. DL measurements have been used in many applications for quality control. However, relatively little research has been reported on dried plant material such as Chinese herbs. The objective of the present study is to establish a protocol for direct and rapid DL measurements of dried Chinese herbal materials, including the determination of the dependence on: (a) the optimal excitation time utilizing a white light source; (b) the optimal size of the grinded herbal particle; and (c) the humidity conditions before and during measurement. Results indicate that stable and reproducible curves of DL photon emission depend mainly on the water content of herbal materials. To investigate the application of the established DL measurement protocol, non-processed and processed Aconitum (Aconitum carmichaelii Debx.), wild and cultivated rhubarb (Rheum palmatum L.) and ginseng (Panax ginseng C.A.Mey) of different ages were measured using DL. The results suggest that DL technology is a potential tool for assessment of dried Chinese herb qualities. The results warrant a further exploration of this technique in relation to therapeutic properties of the herbs. Copyright © 2016 John Wiley & Sons, Ltd.

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Germination Capability of Wheat Seeds in Correlation with Delayed Luminescence Intensity

Abstract: The wheat seeds' delayed luminescence is related to the samples´ germination rate, with inverse relation observed and more clearly evidenced at the end of measurement term (4

Cited by 2 publications

References 6 publications

Relationship between delayed luminescence emission and mitochondrial status in Saccharomyces cerevisiae

Relationship between delayed luminescence emission and mitochondrial status in Saccharomyces cerevisiae

Delayed luminescence: an experimental protocol for Chinese herbal medicines

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