Germination Biology of Picralima nitida(Stapf) under Pretreatments

Abstract

Viable seed sometimes fails to germinate under favourable conditions. The study assessed the germination of Picralima nitida (Stapf) seeds following pretreatments. Fruits were collected from Benin, Southern Nigeria (lat. 6 0 23 1 N and long. 5 0 13 1 E). The seeds were extracted manually and processed for sowing. Seeds were pretreated using ten pretreatment methods: control; soaking in water at 90 0 C and allowing to cool overnight; soaking in water at room temperature; seeds which coats were manually removed; soaking in concentrated sulphuric acid (H2SO4) for 1min; 2mins; and 5mins; and soaking in primarily recycled H2SO4 for 2mins, 5mins and 10mins. One hundred seeds were assigned to each treatment and replicated in two locations of Akungba (lat. 7 0 28 1 N and long. 5 0 46 1 E) and Ibadan (lat. 7 0 24 1 N and long. 3 0 49 1 E). Seeds were sown in germination trays containing washed-sterilized river sand in the screen house. Germination counts were taken daily. P. nitida exhibited "durian-epigeal" germination, seeds which coats were manually removed were the first batch to germinate 17 and 22 days after sowing (DAS) in Ibadan and Akungba respectively. The highest germination percentage of 100 in Ibadan and 84 in Akungba were obtained among this batch. Seeds soaked in water at room temperature gave 86% germination in Akungba and 63% in Ibadan. Seeds soaked in water at 90 0 C, and primarily recycled H2SO4 acid for 10mins did not germinate for 106days. Analysis of variance revealed significant differences at P≤0.05 in treatment and location effects. Mean germination percentage of seeds which coats were removed was significantly different from other treatments. The inherent dormancy in P. nitida seed can be treated by manually removing the seed coat before sowing.

Effect of Watering Regimes and Water Quantity on the Early Seedling Growth of Picralimanitida (Stapf)

“…5º 13'E). Seeds were extracted from the fermented fruits manually and the seed coats removed to hasten germination (Gbadamosi, 2013); thereafter sown in washed river sand. The experiment was set up at the National Centre for Genetic Resources and Biotechnology,Moor plantation;Ibadan,Nigeria (lat.…”

Importance ethnobotanique et valeur d’usage de <i>Picralima nitida</i> (stapf) au Sud-Bénin (Afrique de l’Ouest)

“…Les Goun connaissent mieux et possèdent une meilleure connaissance des usages de l'espèce (Gbadamosi, 2013). Ce travail confirme que les graines, racines, feuilles et écorce de P. nitida sont utilisées par les populations enquêtées dans 21 traitements parmi lesquelles on peut citer l'angine, le paludisme, le diabète, les rituelles comme l'enterrement, le mariage, et la protection.…”

“…Les travaux biochimiques effectués par (Fakeye et al, 2000;Ramirez and García-Rubio, 2003) montrent que l'écorce de la racine et les fruits de P. nitida contiennent de l'akuammigine, de l'akuammicine, de la picracine et de la désacétylpicraline, et les feuilles de l'akuammine, de l'akuammigine, de la picraphylline et de la mélinonine A. Ces alkaloides possèdent une forte activité sympathomimétique et analgésique locale et leurs effets sont comparables à ceux de la cocaïne plus durable que la yohimbine (Fakeye et al, 2004;Erharuyi et al, 2014). Les travaux de germinations effectués sur P. nitida jusque-là sont basés sur des prétraitements et la culture in vitro des graines (Gbadamosi, 2013 …”