Germination and storability of calcium-alginate coated somatic embryos of mango ginger (Curcuma amada Roxb.)

Raju, Chellappan Soundar; Aslam, Abubakker; Shajahan, Appakan

doi:10.1007/s13580-016-0096-7

Cited by 22 publications

(4 citation statements)

References 58 publications

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“…In both explants of S. khuzistanica, we found that using half-strength MS culture media led to the highest regrowth percentage, regrowth rate, and other growth features. The best regrowth frequency of synthetic seeds was also recorded using a half-strength MS culture media in Dendrobium white fairy (Siew et al (Raju et al 2016). In this study, we used two explants, nodal segment and shoot tip, for the production of synthetic seeds in S. khuzistanica, and showed that encapsulating the nodal segments in a 1/2 MS culture medium resulted in the highest regrowth percentage and rate.…”

Section: Discussionmentioning

confidence: 61%

Alginate encapsulation of micro-cuttings in endangered Satureja khuzistanica species: a promising method for obtaining genetically stable plants with high rosmarinic acid content

Asadi

Abdollahi

Moosavi

et al. 2022

Plant Cell Tiss Organ Cult

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Synthetic seed production is a suitable method for the rapid, mass, and uniform proliferation of Satureja khuzistanica Jamzad, a valuable medicinal plant of the Lamiaceae family. In this study, the encapsulation of micro-cuttings of S. khuzistanica was investigated using sodium alginate (SA). In order to determine the best conditions for encapsulation in the S. khuzistanica plant, different concentrations of SA and CaCl 2 .2H 2 O, different types of explant and matrix culture media, different types and concentrations of plant growth regulator, and different cold storage conditions of synthetic seeds were tested. Also, the genetic stability of regenerated plants from synthetic seeds using the ISSR molecular marker as well as the content of rosmarinic acid in synthetic seed derived plants were evaluated in this study. The use of 3% SA and 100 mM CaCl 2 .2H 2 O was found to be the best composition for gel complexation. Encapsulation of nodal segments with 1/2 MS medium containing 2.5 µM BAP resulted in the highest regrowth percentage (72.22%) and regrowth rate (0.173). Pre-culture of nodal segments in 1/2 MS medium containing 5 µM IBA and 0.2% activated charcoal for 10 days and the use of 2.5 µM IBA in the rooting medium resulted in the highest rooting percentage (88.88%) in synthetic seed-derived plants.The highest regrowth percentage (61.11%) and regrowth rate (0.173) of synthetic seeds were obtained using 0.45 mg/L TDZ in MS liquid medium. The placement of germinated seeds on the coco peat substrate resulted in the highest conversion rate (61.11%) of synthetic seeds to the plant. Also, storage of encapsulated nodal segments at 4°C in MS culture medium, compared to cold storage without using MS medium, resulted in better regrowth of synthetic seeds. The highest regrowth percentage (44.44%) and regrowth rate (0.092) for cold-stored synthetic seeds occurred after two weeks. The genetic stability testing by ISSR molecular marker showed that synthetic seed-derived plantlets were genetically similar to their mother plants. Also, encapsulated nodal segments and shoot tip derived plants signi cantly enhanced the rosmarinic acid content up to 7.77 times the natural seed derived plants. Genetic restoration programs, short-term storage, and germplasm distribution in S. khuzistanica plants could all bene t from the encapsulation regeneration strategy reported here.

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Section: Discussionmentioning

confidence: 61%

Alginate encapsulation of micro-cuttings in endangered Satureja khuzistanica species: a promising method for obtaining genetically stable plants with high rosmarinic acid content

Asadi

Abdollahi

Moosavi

et al. 2022

Plant Cell Tiss Organ Cult

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“…Pada Tabel 2 terdapat beberapa bahan tambahan yang dapat digunakan dalam campuran matrik benih sintetik yaitu medium basal, misal medium MS, Woody Plant, dan SH. Bahan tambahan ini sangat penting untuk pertumbuhan tunas dan akar dari embrio somatik yang dienkapsulasi (Raju et al, 2016). Sukrosa digunakan sebagai sumber energi dan menjaga tekanan osmotik yang akan mempengaruhi perkembangan embrio somatik (Roostika et al, 2012).…”

Section: Potensi Aplikasi Embrio Somatik Untuk Pembuatan Benih Sintetikunclassified

Pengaruh Hormon terhadap Induksi Embrio Somatik Kacapiring (Gardenia jasminoides) dan Potensi Aplikasinya dalam Pembuatan Benih Sintetik

Pardede

Sidharta

2021

Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati

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Kacapiring (Gardenia jasminoides) is an ornamental plant that has many uses, as a cut flowers, food ingredients, textile materials, cosmetic raw materials, and containing therapeutic compounds. The high demand for gardenia needs to be balanced with the results of its cultivation. Conventional propagation of gardenias has disadvantages such as a long life cycle, genotypes can differ from the parent, and can carry disease from the parent. Therefore, more efficient gardenia propagation techniques are needed, one of which is by making synthetic seeds. Synthetic seeds can be produced by encapsulating somatic embryos. Somatic embryos are collections of embryonic cells formed from soma cells that have the ability to regenerate into a complete new plant. Hormones are organic materials that occur naturally in plants or are synthetic. Hormones have an effect on plant differentiation and growth. Somatic embryogenesis can be carried out using auxin and cytokinin hormones, because auxins can induce embryogenic callus and cytokinins can initiate the embryogenic process in callus. Synthetic seeds are single somatic embryos covered by a matrix, so that they can be stored for a certain duration of time and can grow into a complete plant in both in vitro and ex vitro conditions. This literature review is to provide an overview based on existing references on the effects of various types of hormones for the induction of gardenia somatic embryos, synthetic seed production methods, and synthetic seed storage temperatures.

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“…To facilitate this phase, somatic embryos, as well as other propagules, can be encapsulated in calcium alginate or other matrixes in order to protect and enable their easier handling (Rai et al, 2009). Capsule hardness, resulting from the concentration of alginate and calcium, has an influence on embryo development and germination (reviewed by Gantait et al, 2015), and needs to be experimentally assessed case by case (e.g., Haque and Ghosh, 2016; Raju et al, 2016; Prakash et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Explant Type, Culture System, 6-Benzyladenine, Meta-Topolin and Encapsulation Affect Indirect Somatic Embryogenesis and Regeneration in Carica papaya L.

2018

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A protocol to propagate papaya hybrid plants through indirect somatic embryogenesis was developed considering the effect of explant type, culture system, particular cytokinins and encapsulation, in different stages of the process. Optimal 2,4-dichlorophenoxyacetic acid (2,4-D) concentrations for non-embryogenic callus formation ranged between 9.0 and 27.1 μM in half-cut seeds, while higher concentrations were harmful. Non-embryogenic callus was also obtained with 22–158 μM 2,4-D from hypocotyl segments. Callus with embryogenic structures was only obtained in half-cut seeds cultured in the darkness on half-strength Murashige and Skoog culture medium supplemented with 2,4-D, while hypocotyl segments and isolated zygotic embryos failed to produce this type of callus regardless of the 2,4-D and sucrose (30 and 70 g l-1) concentrations tested in this study. Both, embryogenic callus development and quantity of somatic embryos formed per embryogenic callus, which ranged between 11 and 31 units after 14 months, required 2,4-D, but without any effect of the concentration. Histological studies confirmed the multicellular origin of the somatic embryos. In further steps, liquid medium induced over four times more somatic embryos than agar-gelled medium and showed significantly higher production of globular somatic embryos (85 vs. 57%). Both, 6-benzyladenine (BA) and meta-topolin (Mtop) stimulated sprouting (40–45%) of the somatic embryos (development of shoots only) in concentrations of up to 2.7 and 10 μM, respectively. Sprouting probability showed a 2nd order polynomial trend despite the range of concentration used for each cytokinin. This is the first report about the positive effect of Mtop on the apical shoot development of Carica papaya somatic embryos known to the authors. Radicle growth was observed in 5% or less of the cultivated embryos, regardless of the BA concentration. Finally, all encapsulation conditions tested (2.5, 3.5, and 4.5% sodium alginate, combined with 50 and 100 mM CaCl2) reduced sprouting of somatic embryos when compared to the non-encapsulated ones, whereas capsule hardness showed low correlation with embryo sprouting. Embryos were further cultivated until they became plantlets approximately 5 cm long. They were acclimatized and afterward planted in the field, where they flowered and produced fruit.

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Germination and storability of calcium-alginate coated somatic embryos of mango ginger (Curcuma amada Roxb.)

Cited by 22 publications

References 58 publications

Alginate encapsulation of micro-cuttings in endangered Satureja khuzistanica species: a promising method for obtaining genetically stable plants with high rosmarinic acid content

Alginate encapsulation of micro-cuttings in endangered Satureja khuzistanica species: a promising method for obtaining genetically stable plants with high rosmarinic acid content

Pengaruh Hormon terhadap Induksi Embrio Somatik Kacapiring (Gardenia jasminoides) dan Potensi Aplikasinya dalam Pembuatan Benih Sintetik

Explant Type, Culture System, 6-Benzyladenine, Meta-Topolin and Encapsulation Affect Indirect Somatic Embryogenesis and Regeneration in Carica papaya L.

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