Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences

Chaiyasut, Chaiyavat; Sivamaruthi, Bhagavathi Sundaram; Pengkumsri, Noppawat; Keapai, Waranya; Kesika, Periyanaina; Saelee, Manee; Tojing, Parichart; Sirilun, Sasithorn; Chaiyasut, Khontaros; Peerajan, Sartjin; Lailerd, Narissara

doi:10.3390/ph10010003

Cited by 31 publications

(20 citation statements)

References 47 publications

(60 reference statements)

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“…After incubation, 20% saturated sodium carbonate was used to stop the reaction, and the absorbance was measured at 725 nm. The phenolic content of the sample was denoted as mg Gallic acid equivalents (GAEs) per ml of the sample [11].…”

Section: Determination Of Total Polyphenolic Contentmentioning

confidence: 99%

Lactobacillus Paracasei Hii01 Mediated Fermentation of Syzygium Cumini L. Fruits: Assessment of Changes in Phenolic Content and Antioxidant Capacity

Sirilun

Sivamaruthi

Kesika

et al. 2018

Asian J Pharm Clin Res

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Objective: Syzygium cumini L. fruits are reported for several pharmacological applications and winemaking. The present study was conducted to develop and analyze the phytochemical content, and antioxidant properties of lactic acid bacteria mediated fermented S. cumini fruit juice (FSJ). Methods:The pH of FSJ was measured by pH meter, and total acidity was calculated by titration method. The Folin-Ciocalteu colorimetric method was employed to assess the total phenolic content (TPC), and antioxidant properties were determined by 2, 2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid, ferric reducing antioxidant power, and ferrous ion-chelating assays. Results:The formula 1 and 2 displayed pH of 3.23, and 3.12, respectively, after 6 months of fermentation. The acidity of F1 and F2 was regularly increased from 0.23 to 0.55 and 0.29 to 0.53 mg lactic acid equivalent per ml sample, respectively. The TPC of FSJ was progressively increased. The F1 and F2 showed an increase in TPC from 0.94 to 1.61 and 1.05 to 1.95 mg Gallic acid equivalent per ml of sample, respectively. The Formula 1 and 2 exhibited antioxidant capacities of 1.90 and 2.06 mg Trolox equivalent antioxidant capacity per ml sample, 0.81 and 0.80 quercetin equivalent antioxidant capacity per ml sample, and 1.67 and 1.82 Vitamin-C equivalents antioxidant capacity per ml sample, respectively. Ferric reducingantioxidant power of Formula 1 and 2, and Control 1 and 2 were 3.90, 4.29, 2.96, and 3.27 mg Fe2SO4 equivalents per ml of sample, respectively. Whereas, the chelating power of samples was not significantly changed at the end of fermentation. Conclusion:The high-quality FSJ was prepared with enhanced antioxidant capacity and phenolic compounds. The probiotic containing FSJ is an alternative functional food supplement to prevent, cure and manage several degenerative disorders. Further, clinical studies are essential to insight the functional quality of FSJ.

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Section: Determination Of Total Polyphenolic Contentmentioning

confidence: 99%

Lactobacillus Paracasei Hii01 Mediated Fermentation of Syzygium Cumini L. Fruits: Assessment of Changes in Phenolic Content and Antioxidant Capacity

Sirilun

Sivamaruthi

Kesika

et al. 2018

Asian J Pharm Clin Res

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“…Previous investigations also revealed that dietary feeding of Keunnunjami rice powder inhibited body weight gain and hyperlipidemia in high-fat diet-fed mice, and diet supplementation of germinated Keunnunjami rice powder improved lipid metabolism in ovariectomized rats [ 24 , 25 ]. Extracts from germinated black rice have also been shown to reduce plasma glucose, insulin, cholesterol, and triglyceride levels in diabetic rats [ 38 ]. Similarly, dietary intake of reddish-brown rice powder improved glucose metabolism in ovariectomized rats, and germinated rice showed a greater hypoglycemic effect than nongerminated rice [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Oral Administration of Germinated, Pigmented, Giant Embryo Rice (Oryza sativa L. cv. Keunnunjami) Extract Improves the Lipid and Glucose Metabolisms in High-Fat Diet-Fed Mice

Chung

Kang

2021

Oxidative Medicine and Cellular Longevity

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Obesity is a significant risk factor for chronic diseases. The effect of ethanol extract from germinated Keunnunjami, blackish-purple rice with a giant embryo, compare to ordinary brown rice, on the body weight and lipid and glucose metabolism in high-fat diet-fed mice was analyzed. Mice were fed with a high-fat diet-fed for 3 weeks and then orally administered with either distilled water (HF) or extract (0.25%, w / w ) from brown, germinated brown, Keunnunjami, and germinated Keunnunjami rice for 4 weeks. Control mice were fed with a normal diet and orally administered with distilled water. The HF group showed markedly higher body weight and triglyceride, cholesterol, fatty acid, glucose, and insulin levels than the control group. However, the oral administration of rice extracts ameliorated this high-fat diet-induced obesity, hyperlipidemia, and hypoglycemia through the modulation of adipokine production, lipogenic and glucose-regulating enzyme activities, and mRNA expression of genes associated with lipid and glucose metabolism. The germinated Keunnunjami extract exhibited greater hypolipidemic, hypoglycemic, and body weight-lowering effects than the other rice extracts. The results demonstrated that germination could further enhance the physiological properties of rice and that germinated Keunnunjami extract has a strong therapeutic potential against high-fat diet-induced obesity, hyperlipidemia, and hyperglycemia.

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“…25-OHVD3 was also measured by rat ELISA kits 24 catalogue no MBS288530 (Mybiosource USA). Furthermore, rat insulin ELISA kit (MBS724709, Mybiosource USA) was used to measure the insulin level, while plasma glucose concentration was assessed by the spectrophotometric method 25 . Additionally, Homeostatic model assessment for insulin resistance (HOMA-IR) and Homeostatic model assessment for beta function (HOMA-IR) were calculated 26 .…”

Section: Methodsmentioning

confidence: 99%

Effects of Testosterone Replacement on Insulin Sensitivity, Blood Glucose, Serum Lipids and Vitamin D Concentration in a Rat Model of Andropause

Rezk¹

2017

IJCEMS

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Abstract:Background: Testosterone facilitates various metabolic processes in the bones, muscles, bone marrow, brain and immune system. Testosterone deficiency may contribute to cardiovascular diseases, central obesity and metabolic dysfunction. Aim: to investigate the effects of testosterone deficiency on blood glucose levels, insulin sensitivity, vitamin D, Homeostatic model assessment of insulin resistance (HOMA-IR), Homeostatic model assessment for beta function (HOMA-IR) and serum lipids in rat models of andropause. Methods, 48 male rats divided into 4 equal groups (n=12/group); a) Control (C) group. b) Orchiectomy (O) group underwent bilateral removal of the testicles. c) Orchiectomy and treated (O+T) group given daily IM testosterone replacement therapy for 7 days, d) Sham and treated (Sh+T) group in which sham operated rats were given the same daily dose of T as O+T group for 7 days. Results: we found that plasma glucose concentration increased significantly (P<0.001) in O group. This increase was corrected by testosterone injection in O+T group. In (Sh+T) group, there is a significant reduction in serum glucose (P<0.001). We also found that insulin level reduced significantly (P<0.001) in O group. This reduction in insulin level was corrected by testosterone. In (Sh+T) group, insulin level increased significantly. 25-OHVD3 was reduced significantly (P<0.05) in O group and increased significantly in O+T group. HOMA-IR level decreased significantly (P<0.001) and this reduction was corrected by Testosterone. HOMA-β reduced significantly (P<0.001) and this reduction was corrected by Testosterone in O+T group. In SH group, there is a marked increase in HOMA-β. We also found insignificant differences between the four groups in serum triglycerides. However, we found very highly significant differences in total cholesterol, LDL and VLDL between C group and O group and very highly significant differences between O+T group and Sh+T group in comparison with O group. We also found significant differences in serum HDL-c in (O) group in comparison with (C) group and between O+T and Sh+T groups in comparison with O group. In conclusion, we found significant reduction in 25-OH(VD), Testosterone, Insulin, HOMA-IR and HOMA-B and significant increase in glucose serum levels in O group and these changes were corrected by Testosterone injection in O+T group. We also found insignificant differences between the four groups in serum triglycerides. However, we found highly significant differences in total cholesterol, LDL, HDL-c and VLDL between C group and O group and very highly significant differences between O+T group and Sh+T group in comparison with O group.

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Germinated Thai Black Rice Extract Protects Experimental Diabetic Rats from Oxidative Stress and Other Diabetes-Related Consequences

Cited by 31 publications

References 47 publications

Lactobacillus Paracasei Hii01 Mediated Fermentation of Syzygium Cumini L. Fruits: Assessment of Changes in Phenolic Content and Antioxidant Capacity

Lactobacillus Paracasei Hii01 Mediated Fermentation of Syzygium Cumini L. Fruits: Assessment of Changes in Phenolic Content and Antioxidant Capacity

Oral Administration of Germinated, Pigmented, Giant Embryo Rice (Oryza sativa L. cv. Keunnunjami) Extract Improves the Lipid and Glucose Metabolisms in High-Fat Diet-Fed Mice

Effects of Testosterone Replacement on Insulin Sensitivity, Blood Glucose, Serum Lipids and Vitamin D Concentration in a Rat Model of Andropause

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