Germinal cell ectopism in the strepsirhine prosimian <i>Galago crassicaudatus crassicaudatus</i>

Yoshinaga, Kazuya; Hess, David L.; Hendrickx, Andrew G.; Zamboni, Luciano

doi:10.1002/aja.1001870302

Cited by 10 publications

(2 citation statements)

References 15 publications

(25 reference statements)

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“…Indeed, extratubular, ectopic germ cells can be found in reasonable number at day 8 p.c. in the rabbit testis (unpublished results; see also the report of Yoshinaga et al, 1990) on intratesticular ectopic germ cells in Galago). On the other hand, a transfer of peritubular cells into the cords remains a possibility which cannot be excluded.…”

Section: Cytodifferentiation Of Somatic Cells Involved Inmentioning

confidence: 72%

Fine structural characteristics of testicular cord formation in the developing rabbit gonad

Wartenberg

Kinsky

Viebahn

et al. 1991

J. Elec. Microsc. Tech.

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This paper presents morphological (light- and electron-microscopical) evidence for the role of the mesonephros in contributing cells to the differentiating indifferent gonad and, after sexual differentiation, to the testis. A continuous process is revealed during which segregation of cells occurs from the developing and regressing mesonephros. Additionally, the complementary role of the coelomic epithelium in gonadal ridge and testis formation is demonstrated. The differentiation of testicular cords, their remodelling from a primary reticulum, and the composition and further change of the cellular content during the period after sexual differentiation is described using a computer-aided three-dimensional reconstruction system. Apart from these morphogenetic events, cytodifferentiation in the somatic cells of the indifferent gonad and of the early differentiated testis is demonstrated using indirect immunofluorescence in combination with monoclonal antibodies to the intermediate filament proteins keratin 8 and 18 and vimentin. The immunohistochemical results show that different forms of cytodifferentiation coexist among the somatic cells present in the indifferent gonad and in the testis early after sexual differentiation.

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Section: Cytodifferentiation Of Somatic Cells Involved Inmentioning

confidence: 72%

Fine structural characteristics of testicular cord formation in the developing rabbit gonad

Wartenberg

Kinsky

Viebahn

et al. 1991

J. Elec. Microsc. Tech.

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“…Apart from being useful for describing the position and development of primordial germ cells at early stages, particularly after experimental manipulations in vitro and in ectopic sites in vivo (see Wartenberg 1989b;Yoshinaga et al 1990;Tam et al 1994), the antibody promises to be helpful in finding a gene specifically expressed in mammalian PGCs. This may in turn help to clarify several important steps in the history of the developing mammalian germ cell, i.e., the separation of the germline from the somatic cell line and the regulation of germ cell proliferation and meiosis.…”

Section: Discussionmentioning

confidence: 99%

Primordial germ cells of the rabbit are specifically recognized by a monoclonal antibody labelling the perimitochondrial cytoplasm

Viebahn

Miething

Wartenberg

1997

Histochemistry and Cell Biology

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Instrumental for studies investigating the development of germ cells, and especially the separation of the germline in the early embryo, are molecular markers which reliably label germ cells and with which regulative factors of germ cell development may be analyzed. Here, we describe the monoclonal antibody PG-2, which is highly specific for the germ cells of the rabbit embryo and labels the perimitochondrial cytoplasm, as demonstrated by immunogold-silver staining. Identical expression patterns are found in germ cells of either sex from early organogenesis at 10 days post-conception (d.p.c.), when the germ cells leave the hindgut epithelium and settle in the gonadal anlage as primordial germ cells (PGCs), until the time immediately prior to birth (30 d.p.c.), when germ cells are either in their oogonial or prospermatogonial state. The antibody is the first to recognize specifically a cytoplasmic epitope in germ cells of a higher vertebrate and may well recognize the mammalian equivalent of the germ plasm found in invertebrates and lower vertebrates. The antibody can be used for early identification of PGCs and may be of help in the elucidation of mammalian germ cell development towards the gonial stages of spermatogenesis and oogenesis.

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Ultrastructural study of mitochondria and their cristae in embryonic rats and primate (N. nemistrina)

1998

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Information on the morphology of mitochondria during embryogenesis is scattered in the literature but there appears to be a developmental pattern characterized by vesiculation of the mitochondrial cristae. During early organogenesis, the embryo is in a relative state of hypoxia and this is associated with decrease of terminal electron transport system activity and a marked increase in glycolysis. Ultrastructural studies of a 14 somite monkey embryo, and day 10 and 12 rat embryos, along with a review of the literature led us to determine that this hypoxic stage is characterized by vesiculation of the mitochondrial cristae. Starting in the late morula stage and continuing during early postimplantation embryogenesis the cristae increase and appear tubular or vesicular. After the end of neurulation, and with onset of vascular perfusion, the cristae gradually become lamellated and by the limb bud stage appear more mature. We suggest that new cristae form from blebs of the inner mitochondrial membrane and that subsequently with maturation these blebs collapse giving them a lamelliform appearance. The delamellated state of the cristae may protect the embryo from toxic respiratory end-products of oxidative respiration which could accumulate in an embryo lacking vascular perfusion. In the heart of monkey and rat embryos, the mitochondria had diameters which were approximately twice those found in skin and neural tube. Anat.

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Germinal cell ectopism in the strepsirhine prosimian Galago crassicaudatus crassicaudatus

Cited by 10 publications

References 15 publications

Fine structural characteristics of testicular cord formation in the developing rabbit gonad

Fine structural characteristics of testicular cord formation in the developing rabbit gonad

Primordial germ cells of the rabbit are specifically recognized by a monoclonal antibody labelling the perimitochondrial cytoplasm

Ultrastructural study of mitochondria and their cristae in embryonic rats and primate (N. nemistrina)

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