(?)-Germacrene D receptor neurones in three species of heliothine moths: structure-activity relationships

Stranden, M.; Liblikas, Ilme; König, Wilfried Α.; Almaas, Tor Jørgen; Borg‐Karlson, Anna‐Karin; Mustaparta, Hanna

doi:10.1007/s00359-003-0434-y

Cited by 70 publications

(50 citation statements)

References 14 publications

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“…Recently, tetramethylrhodamine dextran was used successfully for staining ORNs of single sensilla (Stranden et al, 2003). It seems that the dextran, coupled to biotin in our case, is crucial for transport to the axon terminals of the ORNs.…”

Section: Selective Staining Of Ornsmentioning

confidence: 99%

Multiple olfactory receptor neurons and their axonal projections in the antennal lobe of the honeybee Apis mellifera

Kelber

Rößler

Kleineidam

2006

J of Comparative Neurology

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The poreplate sensilla of honeybees are equipped with multiple olfactory receptor neurons (ORNs), which innervate glomeruli of the antennal lobe (AL). We investigated the axonal projection pattern in glomeruli of the AL (glomerular pattern), formed by the multiple ORNs of individual poreplate sensilla. We used the different glomerular patterns to draw conclusions about the equipment of poreplate sensilla with different ORN types. ORNs of single poreplate sensilla were stained and analyzed by laser-scanning confocal microscopy and 3D software (AMIRA). In 13 specimens we found between 7 and 23 ORNs. This is in accordance with data found in the literature (5-35 ORNs) suggesting that all ORNs of the single poreplate sensilla were stained. The ORNs innervate the AL via all four sensory tracts (T1-T4), and glomeruli of the anterior part of the AL are more often innervated. Each ORN innervates a single glomerulus (uniglomerular), and all ORNs of one poreplate sensillum project to different glomeruli. Visual inspection and individual identification of glomeruli, based on the honeybee digital AL atlas, were used to evaluate mapping of glomeruli by a rigid transformation of the experimental ALs onto a reference AL. ORNs belonging to individual poreplate sensilla form variable glomerular patterns, and we did not find a common organization of glomerular patterns. We conclude that poreplate sensilla are equipped with different ORN types but that the same ORN types can be found in different poreplate sensilla. The equipment of poreplate sensilla with ORNs is overlapping. The mapping of glomeruli by rigid transformation is revealed to be a powerful tool for comparative neuroanatomy.Indexing terms: sensilla placodea; 3D reconstruction; antennal lobe; odor representation; VOI; glomeruliIn many cases, animal behavior is guided by olfaction, and most animals have developed sensory organs and olfactory receptor neurons (ORNs) with remarkable sensitivity to detect and discriminate a vast diversity of different odors. The interface for detecting odors in the environment in vertebrates is the nasal mucosa, with its embedded dendrites of ORNs; in insects the interface is the antenna, with numerous olfactory sensilla and associated ORNs. ORN axons project to the first olfactory neuropil and terminate in glomeruli of the vertebrate olfactory bulb (OB) and the insect antennal lobe (AL). The glomeruli represent functional units of the first olfactory neuropil that receive input from ORNs such that a chemotopic representation emerges. The first olfactory neuropil shares many common design principles across phyla (Hildebrand and Shepherd, 1997;Strausfeld and Hildebrand, 1999).

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Section: Selective Staining Of Ornsmentioning

confidence: 99%

Multiple olfactory receptor neurons and their axonal projections in the antennal lobe of the honeybee Apis mellifera

Kelber

Rößler

Kleineidam

2006

J of Comparative Neurology

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“…For example, Stranden et al (2003) demonstrated structural-activity relationships in the electrophysiological responses of three heliothine moths to the sesquiterpene germacrene D. The selective response of these OSNs to germacrene D was defined by the ten-membered ring system, the position of three double bonds and the position of the isopropyl group. Research on pheromone receptors of the moth Agrotis segetum has also shown that changes in shape and bulkiness, length, position of the double bond or nature of the functional group of the (Z)-5-decenyl acetate molecule (one of the three pheromone components of this species), have an effect, direct or indirect, on the interaction of the molecules with the receptor binding sites.…”

Section: Cpomor19 and Slitor19 Are Tuned To 1-indanonesmentioning

confidence: 99%

A Conserved Odorant Receptor Detects the Same 1-Indanone Analogs in a Tortricid and a Noctuid Moth

et al. 2015

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Odorant receptors (ORs) interface animals with airborne chemical signals. They are under strong selection pressure and are therefore highly divergent in different taxa. Yet, some OR orthologs are highly conserved. These ORs may be tuned to odorants of broad importance, across species boundaries. Two widely distributed lepidopteran herbivores, codling moth Cydia pomonella (Tortricidae) feeding in apples and pears, and the African cotton leafworm Spodoptera littoralis (Noctuidae), a moth feeding on foliage of a wide range of herbaceous plants, both express a receptor ortholog, OR19, which shares 58% amino acid identity and 69% amino acid similarity. Following heterologous expression in the empty neuron system of Drosophila melanogaster, we show by single sensillum recordings that CpomOR19 and SlitOR19 show similar affinity to several substituted indanes. Tests with a series of compounds structurally related to 1-indanone show that 2-methyl-1-indanone, 2-ethyl-1-indanone, 3-methyl-1-indanone, and 1-indanone elicit a strong response from both ORs. A keto group in position 1 is essential for biological activity and so are both rings of the indane skeleton. However, there is an important difference in steric complementary of the indane rings and the receptor. Methyl substituents on the benzene ring largely suppressed the response. On the other hand, alkyl substituents at position 2 and 3 of the five-membered ring increased the response indicating a higher complementarity with the receptor cavity, in both CpomOR19 and SlitOR19. Our results demonstrate a conserved function of an odorant receptor in two moths that are phylogenetically and ecologically distant. It is conceivable that a conserved OR is tuned to signals that are relevant for both species, although their ecological roles are yet unknown. Our finding demonstrates that functional characterization of ORs leads to the discovery of novel semiochemicals that have not yet been found through chemical analysis of odorants from insects and their associated host plants.

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“…With a few exceptions (Wibe et al, 1998;Stranden et al, 2002Stranden et al, , 2003, however, little is known about the enantiospecificity of ORCs responding to nonpheromonal odor compounds. To our knowledge, no study in vertebrates or invertebrates has used intracellular recording to examine the enantioselectivity of PNs innervating identified glomeruli.…”

Section: Introductionmentioning

confidence: 99%

Enantioselectivity of Projection Neurons Innervating Identified Olfactory Glomeruli

Reisenman¹,

Christensen²,

Francke³

et al. 2004

J. Neurosci.

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Projection neurons (PNs) with arborizations in the sexually dimorphic "lateral large female glomerulus" (latLFG) in the antennal lobe (AL) of the moth Manduca sexta previously were shown to respond preferentially to antennal stimulation with (Ϯ)linalool, a volatile compound commonly emitted by plants. In the present study, using intracellular recording and staining techniques, we examined the responsiveness of latLFG-PNs to the enantiomers, (ϩ)linalool and (Ϫ)linalool and found that (1) latLFG-PNs are more responsive to antennal stimulation with (ϩ)linalool than with (Ϫ)linalool, (2) PNs with arborizations in a glomerulus adjacent to the latLFG are preferentially responsive to (Ϫ)linalool, and (3) PNs with arborizations confined to other glomeruli near the latLFG are equally responsive to both enantiomers of linalool. Structure-activity studies showed that the hydroxyl group in this tertiary terpene alcohol is the key feature of the molecule determining the response of enantioselective PNs to linalool. In contrast, the responses of non-enantioselective PNs are less dependent on the alcoholic functionality of linalool. Our findings show that PNs innervating a uniquely identifiable glomerulus respond preferentially to a particular enantiomer of an odor substance. Moreover, PNs with arborizations in a glomerulus adjacent to the latLFG, although less sensitive than latLFG-PNs to linalool, respond preferentially to the opposite enantiomer, demonstrating that information about stimulus-absolute configuration can be encoded in different olfactory glomeruli.

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(?)-Germacrene D receptor neurones in three species of heliothine moths: structure-activity relationships

Cited by 70 publications

References 14 publications

Multiple olfactory receptor neurons and their axonal projections in the antennal lobe of the honeybee Apis mellifera

Multiple olfactory receptor neurons and their axonal projections in the antennal lobe of the honeybee Apis mellifera

A Conserved Odorant Receptor Detects the Same 1-Indanone Analogs in a Tortricid and a Noctuid Moth

Enantioselectivity of Projection Neurons Innervating Identified Olfactory Glomeruli

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