Germ cells and the seminiferous epithelium cycle in the wild rodent Oxymycterus rufus (Rodentia: Cricetidae)

Carolina, Ana; Morais, Tiago; Balarini, Maytê Koch; Menezes, Tatiana Prata; Gomes, Marcos de Lucca Moreira; Morais, Danielle Barbosa; Paula, Tarcízio Antônio Rêgo de; Matta, Sérgio Luís Pinto da

doi:10.9790/3008-1104036171

Cited by 3 publications

(3 citation statements)

References 33 publications

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“…Total duration of spermatogenesis in D . rotundus (37.02 days) was also within the average previously recorded for mammals, varying from 29 to 75 days [22, 24, 44, 45]. We emphasize, however, that the values we obtained for vampire bats were closer to the lower values recorded.…”

Section: Discussionsupporting

confidence: 88%

The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

et al. 2017

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Among all bat species, Desmodus rotundus stands out as one of the most intriguing due to its exclusively haematophagous feeding habits. However, little is known about their spermatogenic cycle. This study aimed at describing the spermatogenic process of common vampire bats through testicular histomorphometric characterization of adult specimens, spermatogenic production indexes, description of stages of the seminiferous epithelium cycle and estimative of the spermatogenic process duration. Morphometrical and immunohistochemical analyzes for bromodeoxiuridine were conducted under light microscopy and ultrastructural analyzes were performed under transmission electron microscopy. Vampire bats showed higher investment in gonadal tissue (gonadosomatic index of 0.54%) and in seminiferous tubules (tubulesomatic index of 0.49%) when compared to larger mammals. They also showed a high tubular length per gram of testis (34.70 m). Approximately half of the intertubular compartment was found to be comprised by Leydig cells (51.20%), and an average of 23.77x106 of these cells was found per gram of testis. The germline cells showed 16.93% of mitotic index and 2.51% of meiotic index. The overall yield of spermatogenesis was 60% and the testicular spermatic reserve was 71.44x107 spermatozoa per gram of testis. With a total spermatogenesis duration estimated at 37.02 days, vampire bats showed a daily sperm production of 86.80x106 gametes per gram of testis. These findings demonstrate a high sperm production, which is commonly observed in species with promiscuous mating system.

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Section: Discussionsupporting

confidence: 88%

The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

et al. 2017

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“…Moreover, the wild rodents herein investigated are classified as opportunistic species and reservoirs of zoonotic pathogens [2,[29][30][31][32][33][34]; understanding their reproductive behavior is very useful to develop conservation management strategies. For instance, the evaluation of reproductive organs in different species allows a better comprehension of distinct reproductive strategies and physiological specificities related to each species [35][36][37][38][39][40][41][42]. This evaluation is of particular interest when one considers that relatively few mammalian species have been investigated, and remarkable differences in their reproductive biology may exist [43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Testis structure, duration of spermatogenesis and daily sperm production in four wild cricetid rodent species (A. cursor, A. montensis, N. lasiurus, and O. nigripes)

2021

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Although rodents represent approximately 40% of all living mammalian species, our knowledge regarding their reproductive biology is still scarce. Due to their high vulnerability to environmental changes, wild rodents have become beneficial models for ecological studies. Thus, we aimed to comparatively investigate key functional testis parameters in four sexually mature wild rodent species (A. cursor, A. montensis, N. lasiurus, and O. nigripes). These species belong to the Cricetidae family, which is the most diverse family of rodents in South America, with a total of ~120 species in Brazil. The results found for the gonadosomatic index and the sickled sperm head shape observed strongly suggest that the species here evaluated are promiscuous, prolific, and short-lived. The duration of spermatogenesis was relatively short and varied from ~35–40 days. Both the percentage of seminiferous tubules (ST) in the testis parenchyma (~95–97%) and the number of Sertoli cells (SC) (~48–70 million) per testis gram were very high, whereas a fairly good SC efficiency (~8–13 round spermatids per SC) was observed. In comparison to other mammalian species studied, particularly the rodents of the suborder Myomorpha (i.e. hamsters, rats and mice), the rodents herein investigated exhibited very high (~62–80 million) daily sperm production per testis gram. This impressive spermatogenic efficiency resulted mainly from the short duration of spermatogenesis and quite high values found for the ST percentage in the testis and the SC number per testis gram. We expect that the knowledge here obtained will help conservation programs and the proper management of wildlife.

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“…The population of Sertoli cells was calculated based on their production per testicle and per gram of testicle, and the corrected numbers of nucleoli of Sertoli cells from the transverse sections and total length of seminiferous tubule, were estimated according to Hochereau- de-Reviers & Lincoln (1978). Testicular spermatic reserve (TSR) was calculated to estimate the total spermatic reserve per gram, based on the histological quantity (Morais et al, 2016). Daily spermatic production (DSP) was calculated as the histological quantity of testicles, according Amann & Almquist (1962).…”

Section: Spermatogenic Yieldmentioning

confidence: 99%

Spermatogenesis in sheep supplemented with detoxified castor bean (Ricinus communis L.) as a replacement for soybean meal

Rocha

Ribeiro

Santana

et al. 2020

Rev. bras. saúde prod. anim.

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The objective was to evaluate the effect of replacing soybean meal with the detoxified castor bean cake on testicular morphometry and spermatogenesis of sheep. Were used 24 uncastrated, 9-month old sheep weighing 29±0.8 kg they were randomly distributed among three treatments: T1 = 0%, T2 = 50%, and T3 = 100% substitution of soybean meal with detoxified castor bean cake. The animals were fed with Aruana grass pastage (Panicum maximum ‘Aruana’) and a ration for 90 days. After slaughtering, the testicles were collected and histological slides were prepared with tissue fragments. The data were evaluated for normality using the Shapiro-Wilk test, and analysis of variance was carried out at 5% level of significance. Substitution of soybean meal with detoxified castor bean cake had no effect on any of the assessed variables at the tested levels (P >0.05). The mean yield of spermatogenesis was 72.91 rounded spermatids per spermatogonium; the mean of total number of germ cells held by a Sertoli cell was 12.09; the mean of the testicular spermatic reserve was 31.82×109 and that per testicular gram was 238.28×106; the mean of daily spermatic production was 3.03×109 and that per testicular gram was 22.69×106; and the total number of Sertoli cells was 4.15×109 and that per testicular gram was 34.51×106. The results show that it is possible to replace 100% of the soybean meal with detoxified castor bean cake in sheep diet without any effects on spermatogenesis; however, it is important to perform seminal evaluations in future studies.

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Germ cells and the seminiferous epithelium cycle in the wild rodent Oxymycterus rufus (Rodentia: Cricetidae)

Abstract: Oxymycterus rufus is a wild rodent that inhabits one of the world hotspots known as the

Cited by 3 publications

References 33 publications

The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

The spermatogenic process of the common vampire bat Desmodus rotundus under a histomorphometric view

Testis structure, duration of spermatogenesis and daily sperm production in four wild cricetid rodent species (A. cursor, A. montensis, N. lasiurus, and O. nigripes)

Spermatogenesis in sheep supplemented with detoxified castor bean (Ricinus communis L.) as a replacement for soybean meal

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