Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells

Gillot, Isabelle; Jehl-Piétri, Chantal; Gounon, Pierre; Luquet, Serge; Rassoulzadegan, Minoo; Grimaldi, Paul A.; Vidal, Frédérique

doi:10.1242/jcs.02430

Cited by 43 publications

(36 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The down-regulation of N-cadherin might perturb the adhesion between Sertoli cells and spermatids, thus result in a primary damage of spermiogenesis in TAM K/K mice. CD36 is a regulator of the phagocytosis of apoptotic germ cells by Sertoli cells (Gillot et al 2005). A significant down-regulation of Cd36 might contribute to the defective phagocytic ability of TAM K/K Sertoli cells.…”

Section: Tam Rtks and Spermatogenesismentioning

confidence: 99%

Functions of TAM RTKs in regulating spermatogenesis and male fertility in mice

Chen¹,

Wang²,

Qi³

et al. 2009

Reproduction

View full text Add to dashboard Cite

Mice lacking TYRO3, AXL and MER (TAM) receptor tyrosine kinases (RTKs) are male sterile. The mechanism of TAM RTKs in regulating male fertility remains unknown. In this study, we analyzed in more detail the testicular phenotype of TAM triple mutant (TAM K/K ) mice with an effort to understand the mechanism. We demonstrate that the three TAM RTKs cooperatively regulate male fertility, and MER appears to be more important than AXL and TYRO3. TAM K/K testes showed a progressive loss of germ cells from elongated spermatids to spermatogonia. Young adult TAM K/K mice exhibited oligo-astheno-teratozoospermia and various morphological malformations of sperm cells. As the mice aged, the germ cells were eventually depleted from the seminiferous tubules. Furthermore, we found that TAM K/K Sertoli cells have an impaired phagocytic activity and a large number of differentially expressed genes compared to wild-type controls. By contrast, the function of Leydig cells was not apparently affected by the mutation of TAM RTKs. Therefore, we conclude that the suboptimal function of Sertoli cells leads to the impaired spermatogenesis in TAM K/K mice. The results provide novel insight into the mechanism of TAM RTKs in regulating male fertility. Reproduction (2009) 138 655-666

show abstract

Section: Tam Rtks and Spermatogenesismentioning

confidence: 99%

Functions of TAM RTKs in regulating spermatogenesis and male fertility in mice

Chen¹,

Wang²,

Qi³

et al. 2009

Reproduction

View full text Add to dashboard Cite

show abstract

“…Testicular cells present unique metabolic characteristics, in part due to the existence of the blood-testis barrier. The Sertoli cells (SCs) are responsible for the production of metabolic precursors essential for germ cells development [20]. These cells show a "Warburg-like effect" metabolism [21], using the external glucose to produce lactate, which is the preferred substrate of developing germ cells [22].…”

Section: Introductionmentioning

confidence: 99%

Impaired expression of testicular androgen receptor and collagen fibers in the testis of diabetic rats under HAART: the role of Hypoxis hemerocallidea

Ismail

Isaac

Offor

et al. 2017

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

introduction. Wide spectrum of alterations associated with highly active antiretroviral therapy (HAART) has been reported. The current study aimed at evaluating the role of Hypoxis hemerocallidea (HH) aqueous extract on the testosterone levels, expression of androgen receptors and collagen fibers in the testes of streptozotocin--nicotinamide-induced diabetic rats under HAART regimen. Material and methods. Sixty two adult male Sprague-Dawley rats (189.0 ± 4.5 g) were divided into eight groups (8 animals in each treatment groups and 6 rats in the control group). Diabetes was induced by a single intraperitoneal injection of nicotinamide (110 mg/kg bw) followed by streptozotocin (45 mg/kg bw) and the animals were then subjected to various treatments with HAART, HH extract or melatonin. At the end of the experiment, blood samples were collected to measure serum testosterone levels. Testes were fixed in buffered formaldehyde and paraffin processed. The expression of androgen receptor (AR) was assessed by immunohistochemistry and collagen fibers were visualized by Masson trichrome staining. results. Serum testosterone level was drastically (p < 0.0001) reduced in all rats with induced diabetes. In the testis of diabetic rats increased collagen fibers deposition with varying derangements in germinal epithelium of spermatogenic layers were observed. Intertubular hemorrhages and absence of spermatozoa were also noted in the testes of diabetic rats subjected to HAART. Reduced immunoexpression of ARs was found in the nuclei of Sertoli cells and the cytoplasm of spermatogonia and spermatocytes in III-IV stages of the seminiferous epithelium cycle of diabetic animals treated with different dosages of HH alone and those treated with HAART concomitantly with melatonin and HH. The expression of ARs was almost negative in the testes of rats treated with HAART alone. Conclusions. Concomitant treatment of rats with aqueous HH extract during the HAART did not change serum testosterone level nor mitigate the altered expression of collagen fibers and androgen receptor resulting from STZ-nicotinamide-induced diabetes. Therefore, anti-diabetic properties of Hypoxis extract require further investigation.

show abstract

“…CD36 is expressed by Sertoli cells mainly during stages where phagocytosis of residual bodies takes place. CD36 is involved in transport and uptake of fatty acids and is key player for phagocytosis and lipid recycling (Gillot et al, 2005). An imbalance in the levels of CD36 is partly responsible for the breakdown of self-tolerance during spontaneous autoimmuno orchitis (Akpovi et al, 2006;.…”

Section: Testicular Cholesterol and Spermatogenesismentioning

confidence: 99%

Surfing the wave, cycle, life history, and genes/proteins expressed by testicular germ cells. Part 5: Intercellular junctions and contacts between germs cells and Sertoli cells and their regulatory interactions, testicular cholesterol, and genes/proteins associated with more than one germ cell generation

Hermo

Pelletier

Cyr

et al. 2009

Microscopy Res & Technique

View full text Add to dashboard Cite

In the testis, cell adhesion and junctional molecules permit specific interactions and intracellular communication between germ and Sertoli cells and apposed Sertoli cells. Among the many adhesion family of proteins, NCAM, nectin and nectin-like, catenins, and cadherens will be discussed, along with gap junctions between germ and Sertoli cells and the many members of the connexin family. The blood-testis barrier separates the haploid spermatids from blood borne elements. In the barrier, the intercellular junctions consist of many proteins such as occludin, tricellulin, and claudins. Changes in the expression of cell adhesion molecules are also an essential part of the mechanism that allows germ cells to move from the basal compartment of the seminiferous tubule to the adluminal compartment thus crossing the blood-testis barrier and well-defined proteins have been shown to assist in this process. Several structural components show interactions between germ cells to Sertoli cells such as the ectoplasmic specialization which are more closely related to Sertoli cells and tubulobulbar complexes that are processes of elongating spermatids embedded into Sertoli cells. Germ cells also modify several Sertoli functions and this also appears to be the case for residual bodies. Cholesterol plays a significant role during spermatogenesis and is essential for germ cell development. Lastly, we list genes/proteins that are expressed not only in any one specific generation of germ cells but across more than one generation.

show abstract

Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells

Cited by 43 publications

References 45 publications

Functions of TAM RTKs in regulating spermatogenesis and male fertility in mice

Functions of TAM RTKs in regulating spermatogenesis and male fertility in mice

Impaired expression of testicular androgen receptor and collagen fibers in the testis of diabetic rats under HAART: the role of Hypoxis hemerocallidea

Contact Info

Product

Resources

About