Germ cell‐specific expression of Cre recombinase using the <i><scp>VASA</scp></i> promoter in the pig

Song, Yuning; Lai, Liangxue; Li, Li; Huang, Yongye; Wang, Anfeng; Tang, Xiaochun; Pang, Daxin; Li, Zhanjun; Ouyang, Hongsheng

doi:10.1002/2211-5463.12005

Cited by 10 publications

(6 citation statements)

References 25 publications

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“…We prepared serial sections of the internal genitalia for histological examination via H&E staining [ 17 ]. The cystic gonad on the left uterine horn contained portions of both the male and female gonadal structures (ovotestis, Figure 2 ), including testicular parenchyma with seminiferous tubules (ST) ( Figure 3 a–d), efferent ducts (ED) ( Figure 3 e,f) and incompletely developed epididymis (EP) with ductus epididymidis (DE) ( Figure 3 g,h).…”

Section: Resultsmentioning

confidence: 99%

CRISPR/Cas9-mediated mosaic mutation of SRY gene induces hermaphroditism in rabbits

Song

Liang

et al. 2018

Bioscience Reports

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Section: Resultsmentioning

confidence: 99%

CRISPR/Cas9-mediated mosaic mutation of SRY gene induces hermaphroditism in rabbits

Song

Liang

et al. 2018

Bioscience Reports

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“…Also, gene expressions of VASA were decreased significantly in the combined treated group (IV).VASA protein is a fundamental part of germplasm, not fully known congregation of RNA and proteins which is essential for the evaluation and function of germ cell. Expression of VASA was evidenced to balance with germ cell fate (Song et al, 2016). Accordingly, the function of VASA is crucial throughout oogenesis and spermatogenesis in matures as well as important during embryogenesis to specify the lineage of gametes.…”

Section: Discussionmentioning

confidence: 98%

Commingle Consumption of Monosodium Glutamate and Aspartame and Potential Reproductive System Affection of Female Albino Rats: Involvement of VASA Gene Expression and Oxidative Stress

Mostafa¹,

Ammar²,

El-Shafei³

et al. 2020

AAVS

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E valuating the safe consumption of food additives is commonly accepted after testing their toxicities through animal studies as there is scarce data from human studies. The main aim of animal studies is to evaluate the additives' potential risks for human toxicity and to establish the conditions for safe use (Lin et al., 1992).Monosodium glutamate is a crystalline powder that is composed mainly of glutamic acid and to some extent of sodium, and water. Glutamate is a non-essential amino research Article Abstract | Monosodium glutamate (MSG) and Aspartame (ASP) are the most commonly used food additives. Despite their wide-spread, their safety was examined and fears of toxic effects were recently expressed. So, this study aimed to assess the separate and commingle effects of monosodium glutamate and aspartame on some reproduction function of adult female albino rats. A total of Thirty-two female rats were divided randomly into 4 equal groups (8 rats/ group). Group I: Control rats were fed on a standard diet. Group II:(MSG) each rat received MSG (400mg/Kg. body weight). Group III: (ASP) each rat administrated ASP (40 mg/Kg. body weight). Group IV: (MSG + ASP) each rat received MSG and ASP at the same previous dosage regimen for the same duration. All the treatment was orally gavaged daily for 30 days. Either MSG or ASP individually or mixed had significant deleterious effects on the ovarian functions of the rats in the order MSG then ASP. Aspartame alone was unable to alter the estrogen level or VASA gene expression and caused no considerable histopathological changes of the ovaries, while the combination of (MS-G+ASP) decreased significantly the levels of estrogen and gene expression and produced destructive histopathological changes in the pituitary and ovarian tissues leading to reproductive dysfunction. This could be explained by MSG and ASP-induced pronounced ovarian redox imbalance evidenced via generating lipid peroxidation with diminishing reduced glutathione. More researches are mandatory to assess the adjuvant impact of monosodium glutamate and aspartame on female reproductive function and antioxidant defense systems.

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“…The Cre/loxP system has been extensively used in mice for lineage tracing, to study gene and cell function, induction, and development of cancer and a large number of Cre-driver lines are available (22). This is in strong contrast to livestock species where very few Cre transgenic animals have been generated, based solely on random integration into the genome, showing expression but not in vivo functionality (23,24). In the two pig lines reported here, the Cre recombinase is under the control of an endogenous promoter ensuring a correct spatial and temporal expression pattern, and in vivo Cre mediated recombination was confirmed.…”

Section: Discussionmentioning

confidence: 99%

The Missing Link: Cre Pigs for Cancer Research

et al. 2021

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The Cre/loxP system is a powerful tool for the generation of animal models with precise spatial and temporal gene expression. It has proven indispensable in the generation of cancer models with tissue specific expression of oncogenes or the inactivation of tumor suppressor genes. Consequently, Cre-transgenic mice have become an essential prerequisite in basic cancer research. While it is unlikely that pigs will ever replace mice in basic research they are already providing powerful complementary resources for translational studies. But, although conditionally targeted onco-pigs have been generated, no Cre-driver lines exist for any of the major human cancers. To model human pancreatic cancer in pigs, Cre-driver lines were generated by CRISPR/Cas9-mediated insertion of codon-improved Cre (iCre) into the porcine PTF1A gene, thus guaranteeing tissue and cell type specific function which was proven using dual fluorescent reporter pigs. The method used can easily be adapted for the generation of other porcine Cre-driver lines, providing a missing tool for modeling human cancers in large animals.

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Germ cell‐specific expression of Cre recombinase using the VASA promoter in the pig

Cited by 10 publications

References 25 publications

CRISPR/Cas9-mediated mosaic mutation of SRY gene induces hermaphroditism in rabbits

CRISPR/Cas9-mediated mosaic mutation of SRY gene induces hermaphroditism in rabbits

Commingle Consumption of Monosodium Glutamate and Aspartame and Potential Reproductive System Affection of Female Albino Rats: Involvement of VASA Gene Expression and Oxidative Stress

The Missing Link: Cre Pigs for Cancer Research

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