GerE-independent expression of cotH leads to CotC accumulation in the mother cell compartment during Bacillus subtilis sporulation

Baccigalupi, Loredana; Castaldo, Gaetano; Cangiano, Giuseppina; Isticato, Rachele; Marasco, Rosangela; Felice, Maurilio De; Ricca, Ezio

doi:10.1099/mic.0.27356-0

Cited by 17 publications

(20 citation statements)

References 27 publications

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“…In this regard, an important precedent is the control of the outer coat protein CotG by the CotE-independent, morphogenetic coat protein SafA, which is likely to be located at the cortex near the interface with the inner coat (44,60). It is noteworthy that CotH has been shown to stabilize cytoplasmic CotC and CotG (2,30,71). This finding raises the possibility that, at least in part, the roles of CotH and CotO that we have described are due to protection against a protease.…”

Section: Discussionmentioning

confidence: 68%

“…This finding raises the possibility that, at least in part, the roles of CotH and CotO that we have described are due to protection against a protease. Stabilization of CotC and CotG takes place in the cytoplasm (2,30,71). This raises the further possibility that CotH and CotO carry out their functions from a cytoplasmic location.…”

Section: Discussionmentioning

confidence: 99%

“…One of the coat proteins assembled under the control of CotE is CotH, which directs deposition of an important coat protein subset (38,41,70). In addition to its role in coat protein assembly, CotH also stabilizes coat proteins, one of which is CotG (2,30,71). CotG, in turn, directs CotB deposition (41,53,71).…”

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confidence: 99%

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Characterization of the Bacillus subtilis Spore Morphogenetic Coat Protein CotO

et al. 2005

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Bacillus spores are protected by a structurally and biochemically complex protein shell composed of over 50 polypeptide species, called the coat. Coat assembly in Bacillus subtilis serves as a relatively tractable model for the study of the formation of more complex macromolecular structures and organelles. It is also a critical model for the discovery of strategies to decontaminate B. anthracis spores. In B. subtilis, a subset of coat proteins is known to have important roles in assembly. Here we show that the recently identified B. subtilis coat protein CotO (YjbX) has an especially important morphogenetic role. We used electron and atomic force microscopy to show that CotO controls assembly of the coat layers and coat surface topography as well as biochemical and cell-biological analyses to identify coat proteins whose assembly is CotO dependent. cotO spores are defective in germination and partially sensitive to lysozyme. As a whole, these phenotypes resemble those resulting from a mutation in the coat protein gene cotH. Nonetheless, the roles of CotH and CotO and the proteins whose assembly they direct are not identical. Based on fluorescence and electron microscopy, we suggest that CotO resides in the outer coat (although not on the coat surface). We propose that CotO and CotH participate in a late phase of coat assembly. We further speculate that an important role of these proteins is ensuring that polymerization of the outer coat layers occurs in such a manner that contiguous shells, and not unproductive aggregates, are formed.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

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Characterization of the Bacillus subtilis Spore Morphogenetic Coat Protein CotO

et al. 2005

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“…However, while the effect of CotG on CotC/CotU has not been studied in detail, their dependency on the expression of cotH is likely to reflect a stability problem. It has previously been proposed that CotH either protects CotC and CotU from a proteolytic cleavage or acts on them in a chaperone-like way (1,9). Therefore, CotH probably controls the assembly of CotC and CotU only indirectly, by regulating their presence in the mother cell cytoplasm.…”

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confidence: 99%

“…A cotH-null mutation prevents CotC and CotU assembly in the coat as well as their accumulation in the mother cell cytoplasm (10). Since a mutation causing cotH overexpression allows CotC and CotU accumulation in the mother cell cytoplasm (1), it has been proposed that CotH acts by stabilizing CotC and CotU in the mother cell cytoplasm (1,10).…”

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confidence: 99%

CotE Binds to CotC and CotU and Mediates Their Interaction during Spore Coat Formation in Bacillus subtilis

et al. 2010

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CotE is a morphogenic protein that controls the assembly of the coat, the proteinaceous structure that surrounds and protects the spore of Bacillus subtilis. CotE has long been thought to interact with several outer coat components, but such interactions were hypothesized from genetic experiment results and have never been directly demonstrated. To study the interaction of CotE with other coat components, we focused our attention on CotC and CotU, two outer coat proteins known to be under CotE control and to form a heterodimer. We report here the results of pull-down experiments that provide the first direct evidence that CotE contacts other coat components. In addition, coexpression experiments demonstrate that CotE is needed and sufficient to allow formation of the CotC-CotU heterodimer in a heterologous host.

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Progress in research and application development of surface display technology using Bacillus subtilis spores

Lin

Yuan

et al. 2020

Appl Microbiol Biotechnol

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Bacillus subtilis is a widely distributed aerobic Gram-positive species of bacteria. As a tool in the lab, it has the advantages of nonpathogenicity and limited likelihood of becoming drug resistant. It is a probiotic strain that can be directly used in humans and animals. It can be induced to produce spores under nutrient deficiency or other adverse conditions. B. subtilis spores have unique physical, chemical, and biochemical characteristics. Expression of heterologous antigens or proteins on the surface of B. subtilis spores has been successfully performed for over a decade. As an update and supplement to previously published research, this paper reviews the latest research on spore surface display technology using B. subtilis. We have mainly focused on the regulation of spore coat protein expression, display and application of exogenous proteins, and identification of developing research areas of spore surface display technology.

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GerE-independent expression of cotH leads to CotC accumulation in the mother cell compartment during Bacillus subtilis sporulation

Cited by 17 publications

References 27 publications

Characterization of the Bacillus subtilis Spore Morphogenetic Coat Protein CotO

Characterization of the Bacillus subtilis Spore Morphogenetic Coat Protein CotO

CotE Binds to CotC and CotU and Mediates Their Interaction during Spore Coat Formation in Bacillus subtilis

Progress in research and application development of surface display technology using Bacillus subtilis spores

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