Gerbera

Deng, Zhanao; Bhattarai, Krishna

doi:10.1007/978-3-319-90698-0_17

Cited by 11 publications

(8 citation statements)

References 56 publications

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“…While the PM resistance has been transferred to new cultivars developed by the University of Florida using the resistance source UFGE 4033, the cultivar development process relied on classical breeding and PM screening. Identification of genes conferring resistance to PM can speed up the development of PM-resistant cultivars using marker-assisted breeding and novel methods of gene transfer [ 31 ]. In this study, transcriptome sequencing was used to profile the leaf transcriptome of two gerbera breeding lines, UFGE 4033 and 06–245-03, that differed in resistance to powdery mildew and to analyze gene expression specifically during the post-inoculation stage when most of the DR genes are expressed in the breeding lines due to exposure to PM.…”

Section: Discussionmentioning

confidence: 99%

Sequencing and analysis of gerbera daisy leaf transcriptomes reveal disease resistance and susceptibility genes differentially expressed and associated with powdery mildew resistance

et al. 2020

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Background RNA sequencing has been widely used to profile genome-wide gene expression and identify candidate genes controlling disease resistance and other important traits in plants. Gerbera daisy is one of the most important flowers in the global floricultural trade, and powdery mildew (PM) is the most important disease of gerbera. Genetic improvement of gerbera PM resistance has become a crucial goal in gerbera breeding. A better understanding of the genetic control of gerbera resistance to PM can expedite the development of PM-resistant cultivars. Results The objectives of this study were to identify gerbera genotypes with contrasting phenotypes in PM resistance and sequence and analyze their leaf transcriptomes to identify disease resistance and susceptibility genes differentially expressed and associated with PM resistance. An additional objective was to identify SNPs and SSRs for use in future genetic studies. We identified two gerbera genotypes, UFGE 4033 and 06–245-03, that were resistant and susceptible to PM, respectively. De novo assembly of their leaf transcriptomes using four complementary pipelines resulted in 145,348 transcripts with a N50 of 1124 bp, of which 67,312 transcripts contained open reading frames and 48,268 were expressed in both genotypes. A total of 494 transcripts were likely involved in disease resistance, and 17 and 24 transcripts were up- and down-regulated, respectively, in UFGE 4033 compared to 06–245-03. These gerbera disease resistance transcripts were most similar to the NBS-LRR class of plant resistance genes conferring resistance to various pathogens in plants. Four disease susceptibility transcripts (MLO-like) were expressed only or highly expressed in 06–245-03, offering excellent candidate targets for gene editing for PM resistance in gerbera. A total of 449,897 SNPs and 19,393 SSRs were revealed in the gerbera transcriptomes, which can be a valuable resource for developing new molecular markers. Conclusion This study represents the first transcriptomic analysis of gerbera PM resistance, a highly important yet complex trait in a globally important floral crop. The differentially expressed disease resistance and susceptibility transcripts identified provide excellent targets for development of molecular markers and genetic maps, cloning of disease resistance genes, or targeted mutagenesis of disease susceptibility genes for PM resistance in gerbera.

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Section: Discussionmentioning

confidence: 99%

Sequencing and analysis of gerbera daisy leaf transcriptomes reveal disease resistance and susceptibility genes differentially expressed and associated with powdery mildew resistance

et al. 2020

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“…Male sterility could also be beneficial in flowers on which pollen production is not desired by consumers, e.g. in cut gerberas (Deng and Bhattarai, 2018).…”

Section: Crispr Genome Editing Technologymentioning

confidence: 99%

Ornamental plant breeding: entering a new era?

Huylenbroeck

Bhattarai

2022

Ornam. Hortic.

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During the last century, plant breeding of crops and ornamentals has become an important business. Mendel’s principles of inheritance of traits laid the foundation of today’s modern genetics. Although intra- and interspecific cross breeding, chromosome doubling and mutation breeding still form the cornerstone of ornamental plant breeding, the speed of plant breeding has significantly increased since Mendel’s time. New genomic tools now offer possibilities for advanced precision breeding and selection towards more difficult to assess or quantitative traits. Today’s ornamental plant breeding is a complicated exercise, characterized by new and rapidly-changing challenges. Partnerships between industry and research will be essential to implement available technologies in minor crops.

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“…The continuous introduction of new Gerbera cultivars with improved and novel traits is important for the global floral industry. Development of new cultivars in Gerbera has predominantly relied on conventional breeding (Deng and Bhattarai, 2018). Increased breeding efficiency is much needed to meet the demands of the floral industry and consumers of new Gerbera cultivars with better disease resistance.…”

Section: Introductionmentioning

confidence: 99%

“…Powdery mildew (PM) is one of the most devastating pathogens infecting many row crops, vegetables, fruits, and ornamental/specialty crops worldwide. Numerous ornamental crops including Gerbera, rose, hydrangea, dahlia, and poinsettia can be severely infected by PM in the absence of resistant cultivars (Price, 1970;Benson et al, 2002;Braun et al, 2002;Deng and Bhattarai, 2018;Li et al, 2018). PM, caused by Podosphaera xanthii (syn.…”

Section: Introductionmentioning

confidence: 99%

Construction of a genome-wide genetic linkage map and identification of quantitative trait loci for powdery mildew resistance in Gerbera daisy

Bhattarai¹,

Sharma²,

Verma³

et al. 2023

Front. Plant Sci.

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Powdery mildew (PM) is a common fungal disease in many important crops. The PM caused by Podosphaera xanthii has been the most challenging problem in commercial Gerbera (Gerbera hybrida) production globally, often leading to severe losses of crop yield and quality. A small number of PM-resistant breeding lines and cultivars have been reported in Gerbera, but the underlying genetics for PM resistance in Gerbera is largely unknown. Scarcity of genomic resources such as genetic linkage maps and molecular markers has severely hindered the effort to understand the genetic basis and locate loci controlling PM resistance in Gerbera. This study aimed to construct a genome-wide genetic linkage map, identify quantitative trait loci (QTL), and molecular markers for PM resistance in Gerbera. A segregating mapping population was developed by crossing PM-resistant and -susceptible Gerbera breeding lines, genotyped by sequencing, and phenotyped for PM resistance. A genome-wide genetic linkage map constructed with 791 single polymorphic site (SNP) markers spans 1912.30 cM across 27 linkage groups (LG) and reaches a density of 1 marker per 2.42 cM. One major consistent QTL was discovered in LG16, explaining more than 16.6% of the phenotypic variance for PM resistance. The QTL was tagged with two flanking SNP markers. The availability of this genetic linkage map will be very useful for locating and tagging QTLs for other important traits in Gerbera, and the newly discovered QTL and SNP markers will enable development of molecular markers for improving Gerbera for resistance to PM.

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Gerbera

Cited by 11 publications

References 56 publications

Sequencing and analysis of gerbera daisy leaf transcriptomes reveal disease resistance and susceptibility genes differentially expressed and associated with powdery mildew resistance

Sequencing and analysis of gerbera daisy leaf transcriptomes reveal disease resistance and susceptibility genes differentially expressed and associated with powdery mildew resistance

Ornamental plant breeding: entering a new era?

Construction of a genome-wide genetic linkage map and identification of quantitative trait loci for powdery mildew resistance in Gerbera daisy

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