Geraniol Is a Potent Inducer of Apoptosis-like Cell Death in the Cultured Shoot Primordia of Matricaria chamomilla

Methylcrotonyl-CoA and geranyl-CoA carboxylases are involved in leucine/isovalerate utilization (Liu) and acyclic terpene utilization (Atu), and are encoded by liuB/liuD and atuC/atuF, in Pseudomonas aeruginosa

“…Geraniol is structurally related to citronellol, and differs only by the presence of an additional double bond. Recently, it was found that geraniol and related compounds have significant effects on eukaryotic and human cells, and are able to induce apoptosis in vitro in pancreatic cancer cells (Burke et al, 1997(Burke et al, , 2002Carnesecchi et al, 2002aCarnesecchi et al, , b, 2004Duncan et al, 2004;Izumi et al, 1999). The intensity and variety of biological activities of monoterpenes and related compounds have not been fully investigated.…”

Molecular Cloning and mRNA Expression of Geraniol-inducible Genes in Cultured Shoot Primordia ofMatricaria chamomilla

“…4) Recently, we reported that geraniol induces apoptosis-like cell death, including DNA and nuclei fragmentation, in cultured shoot primordia of Matricaria chamomilla. 5) When we investigated gene expression in apoptosis-like cell death after geraniol treatment in soybean cells, we found that glutathione S-transferase (GST) mRNA was increased transiently by the treatment of the cells with geraniol. 6) In continuation of our studies on the defense reaction of plant cells to chemical stress, we examined potential regulatory genes relating to responses to geraniol treatment.…”

“…Shoot primordia of M. chamomilla were grown in Murashige and Skoog's medium as previously described 5) and treated with 5 mM geraniol as a chemical stress. The concentration was su‹cient to increase DNA fragmentation.…”

Geraniol-inducible GlutathioneS-Transferase in Cultured Soybean Cells

“…1,2) On the other hand, we puriˆed and characterized a 20-kDa protein (p20) with trypsin inhibitory and GTP-binding activities from the cultured cells of Glycine max (soybean), 3) and have also cloned and characterized the p20 cDNA from soybeans. 4) In the investigation of a function of p20 in the signal transduction of plant cells against chemical stress, we have now found that glutathione S-transferase (GST) is a kind of geraniolresponsive factor in the cultured soybean cells.…”

“…For the treatment experiment, geraniol was added to suspension soybean cells 3) to a concentration of 5 mM 2) and the cultures were incubated at 259 C. At regular intervals, part (0.5 g) of the cultures wasˆltered out and powdered in liquid nitrogen. Total RNA was isolated from the powder with the guanidium thiocyanate-phenol-chloroform extraction method.…”