Geraniin extracted from the rind of Nephelium lappaceum binds to dengue virus type-2 envelope protein and inhibits early stage of virus replication

Ahmad, Siti Aisyah Abdul; Palanisamy, Uma Devi; Tejo, Bimo Ario; Chew, Miaw Fang; Tham, Hong Wai; Hassan, Sharifah Syed

doi:10.1186/s12985-017-0895-1

Cited by 54 publications

(36 citation statements)

References 44 publications

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“…Compounds with antiviral properties against dengue serve as a promising alternative to dengue therapy. In our previous in vitro study, we showed that geraniin inhibits early stage of DENV-2 replication, particularly the attachment process, by binding to the viral E protein, specifically at the Domain III region [28]. In our current study, we further prove the inhibitory potential of geraniin by quantifying the DNA production in geraniin treated-infected Vero cells.…”

Section: Discussionsupporting

confidence: 58%

Efficacy of geraniin on dengue virus type-2 infected BALB/c mice

Ahmad

Palanisamy

Khoo

et al. 2019

Virol J

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Background Dengue continues to be a major international public health concern. Despite that, there is no clinically approved antiviral for treatment of dengue virus (DENV) infections. In this study, geraniin extracted from the rind of Nephelium lappaceum was shown to inhibit the replication of DENV-2 in both in vitro and in vivo experiments. Methods The effect of geraniin on DENV-2 RNA synthesis in infected Vero cells was tested using quantitative RT-PCR. The in vivo efficacy of geraniin in inhibiting DENV-2 infection was then tested using BALB/c mice with geraniin administered at three different times. The differences in spleen to body weight ratio, DENV-2 RNA load and liver damage between the three treatment groups as compared to DENV-2 infected mice without geraniin administration were determined on day eight post-infection. Results Quantitative RT-PCR confirmed the decrease in viral RNA synthesis of infected Vero cells when treated with geraniin. Geraniin seemed to provide a protective effect on infected BALB/c mice liver when given at 24 h pre- and 24 h post-infection as liver damage was observed to be very mild even though a significant reduction of DENV-2 RNA load in serum was not observed in these two treatment groups. However, when administered at 72 h post-infection, severe liver damage in the form of necrosis and haemorrhage had prevailed despite a substantial reduction of DENV-2 RNA load in serum. Conclusions Geraniin was found to be effective in reducing DENV-2 RNA load when administered at 72 h post-infection while earlier administration could prevent severe liver damage caused by DENV-2 infection. These results provide evidence that geraniin is a potential candidate for the development of anti-dengue drug.

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Section: Discussionsupporting

confidence: 58%

Efficacy of geraniin on dengue virus type-2 infected BALB/c mice

Ahmad

Palanisamy

Khoo

et al. 2019

Virol J

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“…The objectives of this study were to investigate the cytotoxicity effect of betacyanin fractions from red pitahaya and red spinach on Vero cells, followed by investigating the antiviral activity of betacyanin fractions against dengue virus serotype 2 (DENV-2) via virus yield inhibition assay and virucidal assay. Only DENV-2 was investigated because it is the most lethal among four serotypes [ 3 ] and it has low efficacy for Dengvaxia vaccine especially for seronegative individuals [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Antiviral activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against dengue virus type 2 (GenBank accession no. MH488959)

Chang

Pong

Hassan

et al. 2020

Access Microbiology

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This study investigated the antiviral activity of betacyanins from red pitahaya ( Hylocereus polyrhizus ) and red spinach ( Amaranthus dubius ) against dengue virus type 2 (DENV-2). The pulp of red pitahaya and the leaves of red spinach were extracted using methanol followed by sub-fractionation and Amberlite XAD16N column chromatography to obtain betacyanin fractions. The half maximum cytotoxicity concentration for betacyanin fractions from red pitahaya and red spinach on Vero cells were 4.346 and 2.287 mg ml −1 , respectively. The half-maximal inhibitory concentration (IC 50 ) of betacyanin fraction from red pitahaya was 125.8 μg ml −1 with selectivity index (SI) of 5.8. For betacyanin fraction from red spinach, the IC 50 value was 14.62 µg ml −1 with SI of 28.51. Using the maximum non-toxic betacyanin concentration, direct virucidal effect against DENV-2 was obtained from betacyanin fraction from red pitahaya (IC 50 of 126.70 μg ml −1 ; 95.0 % virus inhibition) and red spinach (IC 50 value of 106.80 μg ml −1 ; 65.9 % of virus inhibition). Betacyanin fractions from red pitahaya and red spinach inhibited DENV-2 in vitro .

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“…GN is one of the ellagitannins which contain up to 10% of GH. GN is present in different parts of various types of plants and exhibits antiviral activity against HSV-1 and -2 30 , HIV-1 38 , HBV 39 , human enterovirus 71, and dengue virus type-2 40 . However, to date, there has been no report on the specific uses of GN in the inhibition of the NA activity of influenza viruses.…”

Section: Discussionmentioning

confidence: 99%

Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition

Choi

Kim

et al. 2019

Sci Rep

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Influenza viruses are a serious threat to human health, causing numerous deaths and pandemics worldwide. To date, neuraminidase (NA) inhibitors have primarily been used to treat influenza. However, there is a growing need for novel NA inhibitors owing to the emergence of resistant viruses. Geranii Herba ( Geranium thunbergii Siebold et Zuccarini), which is edible, has long been used in a variety of disease treatments in Asia. Although recent studies have reported its various pharmacological activities, the effect of Geranii Herba and its components on influenza viruses has not yet been reported. In this study, Geranii Herba ethanol extract (GHE) and its component geraniin showed high antiviral activity against influenza A strain as well as influenza B strain, against which oseltamivir has less efficacy than influenza A strain, by inhibiting NA activity following viral infection in Madin–Darby canine kidney cells. Thus, GHE and its components may be useful for the development of anti-influenza drugs.

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Geraniin extracted from the rind of Nephelium lappaceum binds to dengue virus type-2 envelope protein and inhibits early stage of virus replication

Cited by 54 publications

References 44 publications

Efficacy of geraniin on dengue virus type-2 infected BALB/c mice

Efficacy of geraniin on dengue virus type-2 infected BALB/c mice

Antiviral activity of betacyanins from red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius) against dengue virus type 2 (GenBank accession no. MH488959)

Antiviral activity of ethanol extract of Geranii Herba and its components against influenza viruses via neuraminidase inhibition

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