Geometric-Optical Model of Digital Holographic Particle Recording System and Features of Its Application

Abstract

The paper proposes an equivalent optical scheme of an in-line digital holographic system for particle recording and a mathematical model that establishes a one-to-one correspondence between the dimensional and spatial parameters of a digital holographic image of a particle and the imaged particle itself. The values of the model coefficients used to determine the real size and longitudinal coordinate of a particle according to its holographic image are found by calibration. The model was tested in field and laboratory conditions to calibrate a submersible digital holographic camera designed to study plankton in its habitat. It was shown that four calibration measurements are sufficient enough to determine the model coefficients, and the developed design of the submersible digital holographic camera makes it possible to perform these measurements during the recording of each hologram. In addition, this neither requires data on the refractive index of the medium with particles nor on the parameters of the optical elements of the scheme. The paper presents the results of marine experiments in the Kara Sea and the Laptev Sea, as well as in fresh water in laboratory conditions and in Lake Baikal. The error in measuring the particle size in seawater without the use of the model is 53.8%, while the error in determining their longitudinal coordinates is 79.3%. In fresh water, the same errors were 59% and 54.5%, respectively. The error in determining the position of a particle with the use of the designed mathematical model does not exceed 1.5%, and the error in determining the size is 4.8%. The model is sensitive to changes in the optical properties of the medium, so it is necessary to perform calibration in each water area, and one calibration is quite sufficient within the same water area. At the same time, the developed design of the submersible holographic camera allows, if necessary, calibration at each holographing of the medium volume with particles.

Analysis of the Image Magnification Produced by Inline Holographic Systems Based on the Double-Sideband Filter

“…Holography is an optical tool widely used for imaging microscopic objects in a wide range of applications and research disciplines [1][2][3][4][5][6][7]. In 1948, Dennis Gabor introduced a novel microscope that utilized the interference between the diffracted wavefront from microscopic objects and the illumination wavefront [8].…”