Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand

Chaiphongpachara, Tanawat; Changbunjong, Tanasak; Laojun, Sedthapong; Sumruayphol, Suchada; Suwandittakul, Nantana; Kuntawong, Kewarin; Pimsuka, Siripong

doi:10.1016/j.heliyon.2022.e11261

Cited by 6 publications

(12 citation statements)

References 50 publications

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“…This relationship pattern of Lutzia mosquitoes is similar to those found in studies that examined three cryptic species of the An. barbirostris complex in Thailand [ 24 ]. Nevertheless, this correlation has no effect on species identification (inter-species investigations) based on the wing shape analyses [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

“…Eighteen landmarks on the wing vein structure of the Lutzia mosquitoes were identified and digitized for the GM analyses ( Figure 3 ). This study’s landmark placements are based on earlier research that successfully identified certain mosquito species [ 23 , 24 , 35 ].…”

Section: Methodsmentioning

confidence: 99%

“…Previously, this technique was applied to identify cryptic species of the Anopheles dirus and the An. barbirostris complexes in Thailand, and it was found that GM had a high level of discriminating efficiency in many member species [ 23 , 24 ]. In addition, this modern technique was also proven to be effective with several insects of medical and veterinary significance [ 25 , 26 , 27 , 28 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Modern Techniques for Species Identification of Lutzia Mosquitoes (Diptera: Culicidae) in Thailand: Geometric Morphometrics and DNA Barcoding

Laojun

Changbunjong

Chaiphongpachara

2023

Insects

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There are four species of Lutzia mosquitoes in Thailand, including Lutzia chiangmaiensis, Lt. fuscana, Lt. halifaxii, and Lt. vorax. The accurate species identification of adult Lutzia mosquitoes based on morphological features requires many body parts, including the abdominal terga and wing. However, species identification is difficult in the case of damaged specimens when some of their morphological character is missing due to transit or gathering in the field. Thus, we evaluated the efficacy of the landmark-based geometric morphometric (GM) approach for the discrimination of Lutzia species in Thailand. In addition, DNA barcoding was also used in parallel with the GM approach to identify the species. Larvae of Lutzia were collected, raised into adults, and identified based on their morphological characteristics. The validated reclassification test results clearly demonstrated that wing shape resulted in a high level of success in identification (correct identifications ranged from 92.50% to 100%); however, based on the DNA barcoding analyses, our results showed that it was poorly effective in identifying Lt. fuscana and Lt. halifaxii based on an overlap between the intraspecific and interspecific divergence. Moreover, our survey results provide updates on the distribution of Lt. chiangmaiensis and Lt. vorax in Thailand. This research will help medical entomologists more efficiently identify mosquitoes in the genus Lutzia, resulting in more effective mosquito control and surveillance.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of Modern Techniques for Species Identification of Lutzia Mosquitoes (Diptera: Culicidae) in Thailand: Geometric Morphometrics and DNA Barcoding

Laojun

Changbunjong

Chaiphongpachara

2023

Insects

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“…In this study, we used XYOM (freely available at (accessed on 4 March 2022)) for GM analysis [ 32 ]. Eighteen anatomical landmarks on the wing veins were digitized ( Figure 2 ) according to criteria used in previous studies [ 17 , 18 , 33 ].…”

Section: Methodsmentioning

confidence: 99%

“…However, this method is limited by the need for complete morphological specimens and practitioner expertise [ 10 ]. Currently, effective alternative methods for species identification include geometric morphometrics and DNA barcoding; both may support the standard method to accurately identify mosquito samples that are morphologically similar [ 16 , 17 , 18 , 19 , 20 , 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

Species Identification of the Major Japanese Encephalitis Vectors within the Culex vishnui Subgroup (Diptera: Culicidae) in Thailand Using Geometric Morphometrics and DNA Barcoding

Saiwichai

Laojun

Chaiphongpachara

et al. 2023

Insects

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Japanese encephalitis (JE) is a viral infection of the brain caused by the Japanese encephalitis virus, which spreads globally, particularly in 24 countries of Southeast Asia and the Western Pacific region. In Thailand, the primary vectors of JE are Cx. pseudovishnui, Cx. tritaeniorhynchus, and Cx. vishnui of the Cx. vishnui subgroup. The morphologies of three mosquito species are extremely similar, making identification challenging. Thus, geometric morphometrics (GM) and DNA barcoding were applied for species identification. The results of cross-validation reclassification revealed that the GM technique based on wing shape analysis had relatively high potential for distinguishing Cx. pseudovishnui, Cx. tritaeniorhynchus, and Cx. vishnui (total performance = 88.34% of correctly assigned individuals). While the DNA barcoding yielded excellent results in identifying these Culex species based on the DNA barcode gap (average intraspecific genetic distance = 0.78% ± 0.39% and average interspecific genetic distance = 6.14% ± 0.79%). However, in the absence of the required facilities for DNA barcoding, GM techniques can be employed in conjunction with morphological methods to enhance the reliability of species identification. Based on the results of this study, our approach can help guide efforts to identify members of the Cx. vishnui subgroup, which will be useful for the effective vector control of JE in Thailand.

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Molecular and morphometric differentiation of secondary filariasis vector Coquillettidia mosquitoes (Diptera: Culicidae) in Thailand

Laojun¹,

Changbunjong²,

Sumruayphol³

et al. 2023

Infection, Genetics and Evolution

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Geometric morphometric and molecular techniques for discriminating among three cryptic species of the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand

Cited by 6 publications

References 50 publications

Evaluation of Modern Techniques for Species Identification of Lutzia Mosquitoes (Diptera: Culicidae) in Thailand: Geometric Morphometrics and DNA Barcoding

Evaluation of Modern Techniques for Species Identification of Lutzia Mosquitoes (Diptera: Culicidae) in Thailand: Geometric Morphometrics and DNA Barcoding

Species Identification of the Major Japanese Encephalitis Vectors within the Culex vishnui Subgroup (Diptera: Culicidae) in Thailand Using Geometric Morphometrics and DNA Barcoding

Molecular and morphometric differentiation of secondary filariasis vector Coquillettidia mosquitoes (Diptera: Culicidae) in Thailand

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