Geometric Control of Cell Alignment and Spreading within the Confinement of Antiadhesive Poly(Ethylene Glycol) Microstructures on Laser-Patterned Surfaces

Strehmel, Christine; Perez-Hernandez, Heidi; Zhang, Zhenfang; Löbus, Axel; Lasagni, Andrés Fabían; Lensen, Marga C.

doi:10.1021/ab5001657

Cited by 12 publications

(8 citation statements)

References 26 publications

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“…PEG is a very popular biomaterial, due to its non-toxicity, non-fouling characteristics, and non-immunogenicity and has been widely used for drug delivery in medicine, in pharmacy, cell biological research, and in industry mainly as care products [31]. Nevertheless, even though PEG hydrogels are renowned for their intrinsically anti-adhesive properties toward proteins or cells, surface modifications such as nano-or micro-sized topography, gradient or patterned elasticity or chemical alterations do allow cell adhesion, as we and others have found [32][33][34][35][36][37][38][39][40].…”

Section: Biomaterialsmentioning

confidence: 99%

Nano- and Micro-Patterning of Gold Nanoparticles on PEG- Based Hydrogels for Controlling Cell Adhesion

Yesildag¹,

Zhang²,

Ren³

et al. 2018

Noble and Precious Metals - Properties, Nanoscale Effects and Applications

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Gold nanoparticles (Au NPs) have unique and tunable size-and shape-dependent optical and chemical properties and little toxicity. In this chapter, we describe results on Au NPs employed as cell-binding entities at biomaterials' interfaces. Hereby, Au NPs with different sizes and shapes were nano-or micro-patterned on the surface of poly(ethylene glycol) (PEG)-based hydrogels by using our recently developed patterning strategies based on soft lithography. These hybrid biomaterials can be applied in various biological or biomedical applications, such as for fundamental cell studies considering adhesion and migration, tissue engineering, drug delivery, or as biosensors by using surface plasmon resonance (SPR) or surface-enhanced Raman spectroscopy (SERS).

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Section: Biomaterialsmentioning

confidence: 99%

Nano- and Micro-Patterning of Gold Nanoparticles on PEG- Based Hydrogels for Controlling Cell Adhesion

Yesildag¹,

Zhang²,

Ren³

et al. 2018

Noble and Precious Metals - Properties, Nanoscale Effects and Applications

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“…We have fabricated topographic, elastic, and chemical patterns on the hydrogels’ surface, both at the micro- and nano-scale and achieved great control over the cellular responses. 34 − 44 …”

Section: Introductionmentioning

confidence: 99%

Micropatterning of Au NPs on PEG Hydrogels Using Different Silanes To Control Cell Adhesion on the Nanocomposites

2018

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Amino-silanization of silica-based substrates has proven to be effective in guiding the immobilization of citrate-stabilized Au NPs in a good, homogeneous fashion. This accomplishment has formed the basis of fabricating micropatterns of Au NPs on such substrates by patterning of oxidized silicon wafers with (3-aminopropyl)trimethoxysilane (amino-silane) using the microcontact printing (μCP) process. This micropattern of amino-silane is used to specifically adsorb Au NPs. To avoid unspecific adsorption to the nonsilanized areas on the silicon wafers, the nonstamped areas were backfilled with self-assembled monolayers of organosilanes, for example, with methyl- or perfluoro-end-groups. Finally, after having fabricated a micropattern of Au NPs on silicon wafers, the Au NP patterns were transferred onto poly(ethylene glycol) hydrogels by our newly developed procedures, and on these nanocomposite materials, controlled cell adhesion has been achieved. Furthermore, these materials are great candidates for plasmon-based biosensor applications and also for various medical applications, such as for drug delivery systems or photothermal therapies.

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“…9 Surface functionalization strategies based on the use of poly(ethylene glycol) (PEG) polymers are extensively studied to impart adhesion resistance. [10][11][12][13][14] Among these are selfassembling monolayers (SAMs), 5,12,15 polymer brushes based on PEG and zwitterionic moieties immobilized on different substrates [16][17][18][19][20] and the covalent introduction of PEG polymers based on click chemistry. 21,22 Moreover, PEG polymers can be anchored at different materials and substrates via the introduction of triblock copolymers, [23][24][25] in a supramolecular fashion by the post-modification of polymeric membranes based on cyclodextrin host-guest chemistry 26 or introduced at the surface of electrospun polyurethane (PU) fibers.…”

Section: Introductionmentioning

confidence: 99%

Introduction of anti-fouling coatings at the surface of supramolecular elastomeric materials via post-modification of reactive supramolecular additives

2017

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Protein repellent coatings have been extensively studied to introduce anti-fouling properties at material surfaces. Here we introduce a covalent anti-fouling coating at the surface of supramolecular ureidopyrimidinone (UPy) based materials introduced via post-modification of reactive UPy-functionalized tetrazine additives incorporated into the supramolecular polymer material. After material formulation, an antifouling coating comprised of bicyclononyne (BCN) functionalized poly(ethylene glycol) (PEG) polymers was reacted. This coating was covalently attached to the surface via a highly selective electron-demand Diels-Alder cycloaddition between tetrazine and BCN. The anti-fouling properties of three different BCN-PEG polymers, mono-functional-PEG-BCN, bi-functional-PEG-BCN and star-PEG-BCN, respectively, were systematically studied. The mono-functional-PEG-BCN showed minor reduction in both protein adsorption and cell adhesion, whereas the bi-functional-PEG-BCN and the star-PEG-BCN polymer coating demonstrated complete anti-fouling performance, both towards protein adhesion as well as cell adhesion. Additionally, a bioorthogonal ligation strategy was performed in culture medium in the presence of cells showing a similar behavior for the three anti-fouling coatings, which indicates that this strategy can be applied for post-modification reactions in a complex environment.

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Geometric Control of Cell Alignment and Spreading within the Confinement of Antiadhesive Poly(Ethylene Glycol) Microstructures on Laser-Patterned Surfaces

Cited by 12 publications

References 26 publications

Nano- and Micro-Patterning of Gold Nanoparticles on PEG- Based Hydrogels for Controlling Cell Adhesion

Nano- and Micro-Patterning of Gold Nanoparticles on PEG- Based Hydrogels for Controlling Cell Adhesion

Micropatterning of Au NPs on PEG Hydrogels Using Different Silanes To Control Cell Adhesion on the Nanocomposites

Introduction of anti-fouling coatings at the surface of supramolecular elastomeric materials via post-modification of reactive supramolecular additives

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