The northern margin of the East Junggar terrane is highly prospective for Fe-Cu-Au mineralization. However, due to the lack of precise mineralization ages, intensive debates regarding ore genesis existed. In this study, pyrite and magnetite Re-Os isotopes are first used to determine the metallogenic ages and possible genetic links with magmatism at the Laoshankou deposit, the most important Fe-Cu-Au deposit in the region. Magnetites from Fe mineralization stage yield an 187 Re/ 188 Os versus 187 Os/ 188 Os isochron age of 391 ± 55 Ma, which is in broad agreement with ages of the Beitashan Formation volcanic rocks (~390 Ma). The low initial 187 Os/ 188 Os ratio of −0.08-0.16, depleted γOs values of −161-27 and low Re/Os (common) indicated a mantle source composition, implying Fe metals are from the Beitashan Formation Fe-rich basalts and basaltic breccias. Pyrites from sulfide mineralization stage yield a weighted average Re-Os model age of 390 ± 17 Ma with a matched 187 Re versus 187 Os r (radiogenic 187 Os) isochron age of 391 ± 9 Ma, consistent with the symbiotic molybdenite Re-Os model age of 383.2 ± 4.5 Ma, indicating a timing of~380 Ma for sulfide-Cu-Au mineralization.The high initial 187 Os/ 188 Os ratio of 0.53-3.6, large positive γOs values of 323-2,754 and higher Re/Os (common) indicated the increase of crustal materials, which is further limited to the Beitashan Formation S-rich marine sedimentary rocks.In summary, based on the significant two-stage mineralization, that is, Fe (~390 Ma) and sulfide-Cu-Au (~380 Ma), and the different nonmagmatic fluid and metal sources, we prefer considering the Laoshankou Fe-Cu-Au deposit as an IOCG-type. KEYWORDS multistage mineralization, ore genesis, Re-Os geochronology, the northern margin of the East Junggar, the Laoshankou Fe-Cu-Au deposit