Geographical Variations in Chromosomes of the Greater Japanese Shrew-Mole, Urotrichus talpoides (Mammalia: Insectivora)

Harada, Masashi; Ando, Akiro; Tsuchiya, Koichiro; Koyasu, Kazuhiro

doi:10.2108/zsj.18.433

Cited by 22 publications

(19 citation statements)

References 21 publications

(22 reference statements)

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“…The diploid chromosome number of D. pilirostris and U. talpoides was confirmed to be 2n = 34, as indicated in previous studies (Hamada and Yosida, 1980;Kawada and Obara, 1999;Harada et al, 2001). The karyotypes presented common features between D. pilirostris and U. talpoides, in which chromosome 1 was the largest metacentric element, chromosome 5 had a secondary constriction adjacent to the centromeric region on its short arm, and the Y chromosome was dot-like (Fig.…”

Section: Karyotypes Of D Pilirostris and U Talpoidessupporting

confidence: 84%

“…Karyological studies in Scalopinae have been performed using conventional and/or differential staining techniques (Tsuchiya and Yosida, 1971;Hamada and Yosida, 1980;Tsuchiya, 1988;Kawada and Obara, 1999;Harada et al, 2001). U. talpoides consists of two chromosomal races, due to a pericentric inversion of chromosome 14.…”

confidence: 99%

“…U. talpoides consists of two chromosomal races, due to a pericentric inversion of chromosome 14. Both races are distributed parapatrically forming a boundary, the "Kurobe-Fuji line", which divides U. talpoides into the western and eastern populations at the central region of Honshu, the mainland of Japan (Harada et al, 2001). According to these cytogenetic analyses, chromosome 14 of U. talpoides is metacentric, and D. pilirostris and the western race of U. talpoides share an almost identical karyotype with a subtelocentric chromosome 14.…”

confidence: 99%

“…According to these cytogenetic analyses, chromosome 14 of U. talpoides is metacentric, and D. pilirostris and the western race of U. talpoides share an almost identical karyotype with a subtelocentric chromosome 14. Thus, the inversion of chromosome 14 might cause the intraspecific differentiation of U. talpoides into two chromosomal races after speciation (Kawada and Obara, 1999;Harada et al, 2001). On the basis of this hypothesis, the pericentric inversion of chro-mosome 14 might not be a cue to advance the speciation within the common ancestor of the two species of shrew-moles (Kawada and Obara, 1999).…”

confidence: 99%

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Karyotypic evolution and organization of the highly repetitive DNA sequences in the Japanese shrew-moles, Dymecodon pilirostris and Urotrichus talpoides

Nakata

Yoshimura²,

Kuro-o³

et al. 2005

Cytogenet Genome Res

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The karyological relationship and organization of highly repetitive DNA sequences in Japanese shrew-moles were studied by zoo-blot hybridization and fluorescence in situ hybridization (FISH). When the genomic DNA of the eastern race of Urotrichus talpoides was digested with PstI, three fragments of highly repetitive DNA sequences, approximately 0.7, 0.9, and 1.4 kb in length, were observed as distinct bands. The results of FISH in the eastern race of U. talpoides using these three fragments separately as probes showed that the 0.7-kb PstI fragment was distributed in the centromeric regions of most chromosomes, and that the 0.9- and 1.4-kb fragments were predominantly located in the C-heterochromatin region of chromosome 13p. Although the western race of U. talpoides also had three PstI fragments, 0.9- and 1.4-kb PstI fragments were more ambiguous than those of the eastern race. The PstI- digested genomic DNA in Dymecodonpilirostris produced only a faint 0.9-kb band, and its signal patterns obtained by zoo-blot hybridization were clearly different from those of U. talpoides. The 0.7-kb fragment of U. talpoides hybridized strongly with the 0.9-kb fragment of D. pilirostris. In a FISH analysis, the 0.9-kb fragment of D. pilirostris hybridized with highly repetitive DNA in the centromeric regions of most chromosomes from both D. pilirostris and U. talpoides. Zoo-blot hybridization and FISH analyses suggest that the 0.9- and 1.4-kb PstI fragments were generated specifically in the genome of U. talpoides after the common ancestor differentiated into two extant shrew-mole species. A difference in the length of the centromeric elements between U. talpoides and D. pilirostris might be observed due to certain modifications of the repeating unit.

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Section: Karyotypes Of D Pilirostris and U Talpoidessupporting

confidence: 84%

confidence: 99%

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Karyotypic evolution and organization of the highly repetitive DNA sequences in the Japanese shrew-moles, Dymecodon pilirostris and Urotrichus talpoides

Nakata

Yoshimura²,

Kuro-o³

et al. 2005

Cytogenet Genome Res

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“…As determined by cytochrome b mtDNA and nuclear recombination activating gene-1 (RAG1) sequence analyses, the greater and lesser Japanese shrew moles are closely related, but their evolutionary origins and biogeography remain unresolved (31,32). The existence of two distinct chromosomal races of U. talpoides, geographically separated by the Fuji and Kurobe rivers in central Honshu (33,34), provides an opportunity to further clarify the evolutionary origins of shrew mole-borne hantaviruses in Japan. Studies, now underway, will examine whether ASAV is harbored by U. talpoides in locations east of Mie Fig.…”

Section: Discussionmentioning

confidence: 99%

Molecular phylogeny of a newfound hantavirus in the Japanese shrew mole ( Urotrichus talpoides )

Arai

Ohdachi

Asakawa

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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Recent molecular evidence of genetically distinct hantaviruses in shrews, captured in widely separated geographical regions, corroborates decades-old reports of hantavirus antigens in shrew tissues. Apart from challenging the conventional view that rodents are the principal reservoir hosts, the recently identified soricidborne hantaviruses raise the possibility that other soricomorphs, notably talpids, similarly harbor hantaviruses. In analyzing RNA extracts from lung tissues of the Japanese shrew mole (Urotrichus talpoides), captured in Japan between February and April 2008, a hantavirus genome, designated Asama virus (ASAV), was detected by RT-PCR. Pairwise alignment and comparison of the S-, M-, and L-segment nucleotide and amino acid sequences indicated that ASAV was genetically more similar to hantaviruses harbored by shrews than by rodents. However, the predicted secondary structure of the ASAV nucleocapsid protein was similar to that of rodent-and shrew-borne hantaviruses, exhibiting the same coiledcoil helix at the amino terminus. Phylogenetic analyses, using the maximum-likelihood method and other algorithms, consistently placed ASAV with recently identified soricine shrew-borne hantaviruses, suggesting a possible host-switching event in the distant past. The discovery of a mole-borne hantavirus enlarges our concepts about the complex evolutionary history of hantaviruses.host switching ͉ talpid ͉ evolution ͉ Japan

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Colonization history of the Japanese water shrewChimarrogale platycephala, in the Japanese Islands

Iwasa

Abe²

2006

Acta Theriol

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Ja pan con sists of four main is lands: Hokkaido, Hon shu, Shikoku, and Kyushu, and the pe riph eral small is lands (Fig. 1). These is lands have many al pine re gions show ing top o graph ical com plex i ties. Fur ther more, the cli mates of the Jap a nese Is lands vary from north to south, from east to west, and from lower al ti tudes to higher ones. Such geo log i cal fea tures with clima tic vari a tions in the Jap a nese Is lands act as geo graphic bar ri ers and have split small mammal dis tri bu tions. For ex am ple, there are lo cal relic pop u la tions of small ter res trial mam mals, such as the Azumi shrew (Sorex hosonoi), moles (Euroscaptor mizura and Mogera imaizumii), the Jap a nese lesser shrew-mole (Dymecodon [29] Acta Theriologica 51 (1): 29-38, 2006. PL ISSN 0001-7051 Colonization history of the Japanese water shrew Chimarrogale platycephala, in the Japanese Islands Masahiro A. IWASA and Hisashi ABE Iwasa M. A. and Abe H. 2006. Col o ni za tion his tory of the Jap a nese wa ter shrew Chimarrogale platycephala, in the Jap a nese Is lands. Acta Theriologica 51: 29-38.Lab o ra tory of Wild life Sci ence, De part ment of An i mal Sci ence and Re sources, Col lege of Bioresource Sci ences, Nihon Uni ver sity, Fujisawa, Kanagawa 252-8510, Ja pan, e-mail: an der son@brs.nihon-u.ac.jp (MAI); Katsuraoka 26-17, Otaru, Hokkaido 047-0264, Ja pan (HA)

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Geographical Variations in Chromosomes of the Greater Japanese Shrew-Mole, Urotrichus talpoides (Mammalia: Insectivora)

Cited by 22 publications

References 21 publications

Karyotypic evolution and organization of the highly repetitive DNA sequences in the Japanese shrew-moles, <i>Dymecodon pilirostris</i> and <i>Urotrichus talpoides</i>

Karyotypic evolution and organization of the highly repetitive DNA sequences in the Japanese shrew-moles, <i>Dymecodon pilirostris</i> and <i>Urotrichus talpoides</i>

Molecular phylogeny of a newfound hantavirus in the Japanese shrew mole ( Urotrichus talpoides )

Colonization history of the Japanese water shrewChimarrogale platycephala, in the Japanese Islands

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