Geographical distribution and molecular epidemiology of cutaneous leishmaniasis in Fars Province, southern Iran

Davoodi, T; Khamesipour, Ali; Shahabi, Saeed; Gholizadeh, Fatemeh; Pourkamal, Daniel; Hatam, Gholamreza

doi:10.1007/s11356-021-15865-4

Cited by 7 publications

(11 citation statements)

References 25 publications

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“…For amplifcation of a specifc fragment of the kDNA gene in Leishmania, the two primers of LIN4R (F): 5′-GGG GTT GGT GTA AAA TAG GG -3′ and LIN17 (R): 5′-TTT GAA CGG GAT TTC TG -3′ were used. Te primers amplifed 650-bp and 760-bp kDNA fragments of L. major and L. tropica, respectively [7].…”

Section: Dna Extraction and Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

“…Fars Province, located in the south of Iran, is one of the main foci of visceral and cutaneous leishmaniasis. Based on a recent study about the geographical distribution and molecular epidemiology of CL in Fars Province [7], both anthroponotic cutaneous leishmaniasis (ACL) and zoonotic cutaneous leishmaniasis (ZCL) were detected in Shiraz city, while in most other counties of the Province only ZCL has been reported based on molecular data [7]. Terefore, Shiraz city, which has a population of over 1.5 million people, is of special importance as one of the foci of leishmaniasis.…”

Section: Introductionmentioning

confidence: 99%

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Calomyscid Rodents (Rodentia: Calomyscidae) as a Potential Reservoir of Zoonotic Cutaneous Leishmaniasis in a Mountainous Residential Area in the Plateau of Iran: Inferring from Molecular Data of kDNA and ITS2 Genes of Leishmania Major

Shahabi

Azizi

Asgari

et al. 2023

Journal of Tropical Medicine

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Cutaneous leishmaniasis (CL), a neglected tropical disease, is an important health problem in Fars Province, southern Iran. Fars, the fourth most populous Province in Iran, is the center of both anthroponotic and zoonotic cutaneous leishmaniasis (ZCL). Rodents, the reservoir of Leishmania major, play an important role in transmitting ZCL. In the present study, we report Leishmania infection in calomyscid rodents for the first time in mountainous residential areas of Shiraz, the capital of Fars Province, in southern Iran. Rodents were trapped in urban mountainous areas. The skin, liver, and spleen of rodents were examined microscopically for Leishmania infection. In addition, DNA was extracted from the tissues and they were evaluated for Leishmania infection by targeting the kDNA and subsequent sequencing of the nuclear rDNA internal transcribed spacer two (ITS2) region. DNA of L. major was detected in the spleen and liver of calomyscid rodents. Molecular evolution based on DNA-sequencing of the ITS2 gene confirmed the taxonomic situation of the parasite as L. major. Our findings suggest the eco-epidemiological importance of calomyscid rodents in the foci of leishmaniasis in the mountainous residential area on the plateau of Iran. These rodents may play a role in the transmission of leishmaniasis in a residential area and could be considered a potential reservoir for CL.

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Section: Dna Extraction and Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Calomyscid Rodents (Rodentia: Calomyscidae) as a Potential Reservoir of Zoonotic Cutaneous Leishmaniasis in a Mountainous Residential Area in the Plateau of Iran: Inferring from Molecular Data of kDNA and ITS2 Genes of Leishmania Major

Shahabi

Azizi

Asgari

et al. 2023

Journal of Tropical Medicine

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“…The agent of CL is L. major in rural areas and L. tropica in urban areas. CL remains an important disease and widely distributed in Iran and has been reported in central, west, southwest, south, southeast, east, and northeast region ( 7 ). It has been reported in 25 of 31 Iran provinces.…”

Section: Introductionmentioning

confidence: 99%

Cutaneous Leishmaniasis Lesion on the Ear from Kashan, Central Iran: A Case Report

Hooshyar¹,

Rasti²,

Rostamkhani³

2023

IJPA

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Cutaneous leishmaniasis (CL) frequently occurs in many rural and urban areas of Iran. Leishmania major and L. tropica are principally two causative agents of CL in Iran. We report here a case of leishmaniasis of the ear in a 61-year-old man referred to the Reference laboratory, Kashan, central Iran, in Jan 2022. He suffered from a 2-month history of a 1×3 cm lesion on the left ear. In the microscopy examination, amastigotes forms of Leishmania spp. were observed. L. tropica was confirmed using a single PCR with species-specific primers. The patient was introduced to a physician to begin the treatment protocol. It is recommended that physicians, especially in an endemic area, investigate any atypical lesion for CL.

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“…CL holds particular significance in Fars Province, located in southern Iran. This region has consistently reported a high number of cases, making it one of the most affected areas in the country [ 8 , 17 , 18 ]. The prevalence of the disease in Fars Province is attributed to several factors, including the suitable climate and environmental conditions that favor the breeding and survival of sandflies and the vector responsible for transmitting the parasite [ 17 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Leishmania major Infection in Synanthropic Rodents: Evidence for the Urbanization of Zoonotic Cutaneous Leishmaniasis (ZCL) in Southern Iran

Shahabi,

Azizi,

Asgari

et al. 2024

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Cutaneous leishmaniasis is of particular importance in southern Iran. This study aimed to investigate the infection of rodents with Leishmania major in an urban area of Fars Province, located in southern Iran. Rodents were trapped and samples from the liver, spleen, and skin were collected. Impression smears were prepared from these tissues and any skin lesions and were examined microscopically. In addition, a portion of the samples were preserved for subsequent DNA extraction. A total of 41 rodents belonging to three species were caught from 10 trapping stations in gardens or houses within the area. The caught rodent species were Rattus rattus (n = 25, 60.97%), Mus musculus (n = 15, 36.58%), and Meriones persicus (n = 1, 2.5%). Leishmania amastigotes were seen in the spleen tissue smear of 6 (2.43%) of the rodents, including 4 of R. rattus and 2 of M. musculus. Skin lesions were observed on the muzzles of two R. rattus and one M. musculus. Samples taken from these lesions tested positive for Leishmania infection. Leishmania DNA was detected in 18 (43.9%) rodents, including 11 R. rattus, 6 M. musculus, and one M. persicus, based on DNA sequencing of the ITS2 gene and PCR of the kDNA. Phylogenetic reconstruction revealed that the parasite infecting the rodents was L. major. The detection of Leishmania infection in these rodents in urban areas raises concerns about the urbanization of cutaneous leishmaniasis caused by L. major. This urbanization poses unique challenges for control and prevention efforts.

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Geographical distribution and molecular epidemiology of cutaneous leishmaniasis in Fars Province, southern Iran

Cited by 7 publications

References 25 publications

Calomyscid Rodents (Rodentia: Calomyscidae) as a Potential Reservoir of Zoonotic Cutaneous Leishmaniasis in a Mountainous Residential Area in the Plateau of Iran: Inferring from Molecular Data of kDNA and ITS2 Genes of Leishmania Major

Calomyscid Rodents (Rodentia: Calomyscidae) as a Potential Reservoir of Zoonotic Cutaneous Leishmaniasis in a Mountainous Residential Area in the Plateau of Iran: Inferring from Molecular Data of kDNA and ITS2 Genes of Leishmania Major

Cutaneous Leishmaniasis Lesion on the Ear from Kashan, Central Iran: A Case Report

Leishmania major Infection in Synanthropic Rodents: Evidence for the Urbanization of Zoonotic Cutaneous Leishmaniasis (ZCL) in Southern Iran

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