Geographical classification of <i>Epimedium</i> based on HPLC fingerprint analysis combined with multi‐ingredients quantitative analysis

Xu, Ning; Zhou, Guofu; Li, Xiaojuan; Lu, Heng; Meng, Fanyun; Zhai, Huaqiang

doi:10.1002/bmc.3871

Cited by 11 publications

(3 citation statements)

References 38 publications

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“…EBM is a traditional Chinese medicine, mainly used to treat various diseases such as sexual dysfunction and osteoporosis [17][18][19]. Furthermore, due to the protective effects of EBM on the reproductive system, immunity, cardiovascular, bone and nervous system, EBM has been added to various beverages in China.…”

Section: Discussionmentioning

confidence: 99%

Network Pharmacology-based Research of the Therapeutic Effects of Epimedium Brevicornu Maxim on Diminished Ovarian Reserve

He¹,

Yan²,

Jiang³

et al. 2020

Preprint

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Background: Diminished ovarian reserve (DOR) is a common gynecological disease. As a traditional Chinese medicine, Epimedium brevicornu maxim (EBM) is often used to treat sexual dysfunction and irregular menstruation. But whether EBM could alleviate the symptoms of DOR is unclear.Methods: We constructed the DOR rat models and determined the effect of EBM on the development of DOR. Next, we used the network pharmacology to analyze the active ingredients of EBM and the targeted genes of these ingredients. And the critical genes were selected for the next research. ELISA were performed to detect the expression of these genes in the serum of rats.Results: We found that the EBM treatment suppressed the secretion of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in the ovary tissue of rats. Meanwhile, the levels of estradiol (E2), anti-mullerian hormone (AMH) and Inhibin B (INHB) was promoted after the EBM treatment. The HE staining showed that the treatment of EBM enhanced the formation of follicles. Based on the network pharmacology analysis, we found that EBM has the potential to relieve symptoms of DOR by modulating the levels of steroid hormone receptor.Conclusion: In conclusion, EBM relieved the symptoms of DOR by modulating the steroid hormone receptors, such as promoting the secretion of androgen receptor (AR) and estrogen receptor (ESR1).

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Section: Discussionmentioning

confidence: 99%

Network Pharmacology-based Research of the Therapeutic Effects of Epimedium Brevicornu Maxim on Diminished Ovarian Reserve

He¹,

Yan²,

Jiang³

et al. 2020

Preprint

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show abstract

“…Some isolated studies indicated that the compounds of Epimedium from neighboring locations were similar (Huang et al, 2007;Xu et al, 2013;Xu et al, 2017). This study included samples from a wide geographical area and from different species (Table 1; Figure 1), as a way to offer a more comprehensive view of how location and species may affect biomass quality.…”

Section: Pca Analysismentioning

confidence: 99%

HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species

Lima

Nie

et al. 2021

Front. Pharmacol.

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The growth location and plant variety may influence the active components and biological activities of plants used in phytomedicine. In this study, nine sets of different Epimedii Folium, from different representative cultivation locations and Epimedium species, were collected for comparison, using HPLC-DAD combined with multivariate analysis. The objective was to investigate the influence of geographical origin and Epimedium species on the quality of Epimedii Folium, and provide applicable guidance for cultivation and quality control of Epimedii Folium. Several Epimedium spp. sets were used to establish the HPLC-DAD fingerprints and 91 peaks (compounds) were selected for the multivariate analysis. Major compounds were analyzed by HPLC-DAD combined with principal component analysis (PCA). HPLC quantitative analysis of known bioactive compounds was performed. Application of PCA to HPLC data showed that Epimedium samples sharing the same geographical origin or species clustered together, indicating that both species and geographical origin have impacts on the quality of Epimedii Folium. The major bioactive flavonoid compounds, epimedin C, icariin and baohuoside I, were identified and quantified. The concentration of bioactive compounds was significantly influenced both by species and geographical origin. E. sagittatum from Sichuan showed the highest content of bioactive compounds. The results showed that both Epimedium species and geographical origin have strong impact into quality of Epimedii Folium. HPLC data combined with multivariate analysis is a suitable approach to inform the selection of cultivation areas and choose Epimedium spp. most suitable for different geographical areas, resulting in improved quality of Epimedii Folium.

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“…[10][11][12] Herba Epimedii contains various active ingredients [13][14][15] which dominantly include Epimedin C, Icariin > Epimedin B > Baohuoside I, Epimedin A. 16 However, the toxicity shows Epimedin C > Baohuoside I, Icariin, Epimedin A, Epimedin B as revealed by studies on laval and adult zebrafish. 17 Given the advantages of high content and hypotoxicity, Epimedin B might be a promising component to ameliorate osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

The mechanism of Epimedin B in treating osteoporosis as revealed by RNA sequencing‐based analysis

Diao

Wang

Zhou

et al. 2021

Basic Clin Pharma Tox

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With the ageing of populations, the management of osteoporosis is a priority of society in general. Epimedin B, a major ingredient of Herba Epimedii, which has the advantages of high content and hypotoxicity has been proved to be effective in preventing osteoporosis in vitro. However, the efficacy and mechanism of Epimedin B on osteoporosis in vivo have not been well elucidated yet. This study aimed to investigate the effects and the potential mechanisms of 8-week repeated oral administration of Epimedin B (10 and 20 mg/ kg/day) on a mouse osteoporosis model. Effects of Epimedin B were evaluated by examinations of serum bone turnover markers, bone mineral density, bone microstructure parameters and histopathological section. Epimedin B significantly rose N-terminal propeptide of type I procollagen (P1NP) and dropped C-telopeptide of type I collagen (CTX1). Connectivity density (Conn.D) increased significantly while structure model index (DA) decreased significantly after treated by Epimedin B. Meanwhile, Epimedin B administration significantly increased the number of trabecular bones while significantly decreased the gap between them. Overall, Epimedin B showed beneficial effects on osteoporosis. Furthermore, RNA sequencing-based analysis revealed 5 significantly down-regulated transcripts and 107 significantly up-regulated transcripts between the Epimedin B administration group and the model group. These transcripts were mapped to 15 pathways by KEGG enrichment analysis, of which PI3K-Akt signalling pathway, MAPK signalling pathway and PPAR signalling pathway were most connected to osteoporosis. To conclude, Epimedin B is effective in treating osteoporosis in mice via regulating PI3K-Akt, MAPK and PPAR signalling pathway.

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Geographical classification of Epimedium based on HPLC fingerprint analysis combined with multi‐ingredients quantitative analysis

Cited by 11 publications

References 38 publications

Network Pharmacology-based Research of the Therapeutic Effects of Epimedium Brevicornu Maxim on Diminished Ovarian Reserve

Network Pharmacology-based Research of the Therapeutic Effects of Epimedium Brevicornu Maxim on Diminished Ovarian Reserve

HPLC-DAD Fingerprints Combined With Multivariate Analysis of Epimedii Folium From Major Producing Areas in Eastern Asia: Effect of Geographical Origin and Species

The mechanism of Epimedin B in treating osteoporosis as revealed by RNA sequencing‐based analysis

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