Geographic variation of the predictive values of genomic mutations associated with streptomycin resistance inMycobacterium tuberculosis

Dobner, Petra; Bretzel, Gisela; Rüsch-Gerdes, Sabine; Feldmann, Knut; Rifai, Mohammed; Löscher, Thomas; Rinder, Heinz

doi:10.1006/mcpr.1996.0086

Cited by 31 publications

(32 citation statements)

References 13 publications

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“…The prevalence of rpsL codon 43 substitutions in SM resistant M. tuberculosis can be estimated from eight previous reports, in each of which 25 or more SM resistant isolates (cultures) were examined. 4,[6][7][8][9][10][11][12] In total, the mutation was found in 146 (42%) of 345 SM resistant cultures. It was not found in any of the 228 SM sensitive isolates investigated.…”

Section: Resultsmentioning

confidence: 99%

“…It has been noted earlier that there is a geographic variation in the prevalences of mutations associated with SM resistance. 4 Keeping this in mind, it appears safe to say that, at least on average, approximately one in three SM resistant isolates can be expected to have a rpsL codon 43 substitution.…”

Section: Resultsmentioning

confidence: 99%

“…The outer primers MYC-21 (5Ј-CGC TGC AGC AGC TGG T(A,C)C GCA A-3Ј) and MYC-22 (5Ј-CGG AAT TCG GTT CTT (G,C)AC ACC CTG-3Ј) have been described previously. 4 The inner primers MYC-36 (5Ј-AAG GTC AAG ACC GCG GC-3Ј) and MYC-37 (5Ј-TGA TCT TGT AGC GCA CAC-3Ј) were constructed to asymmetrically encompass codon 43 (AAG) with its overlapping MboII restriction enzyme site (GAA-GA). 5 For the first PCR, 1 l each of 50 M solutions of the outer primers MYC-21 and MYC-22, and for the second (nested) PCR equal amounts and concentrations of the inner primers MYC-36 and MYC-37 were used.…”

Section: Methodsmentioning

confidence: 99%

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Rapid, simple, and culture-independent detection of rpsL codon 43 mutations that are highly predictive of streptomycin resistance in Mycobacterium tuberculosis.

Mieskes

Rüsch-Gerdes²,

Truffot-Pernot³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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Abstract. The substitution of codon 43 in the gene rpsL is the single most common mutation found in streptomycin-resistant Mycobacterium tuberculosis. The characterization of this mutation has been hampered by the need for prior cultivation of the mycobacteria, the need for DNA sequencing, or both. In this report we describe a simple and culture-independent technique to detect this mutation directly from sputum samples, requiring little more than a polymerase chain reaction (PCR) machine and a simple agarose minigel. There is no need for labeled probes or DNA sequencing. In a preliminary test of feasibility, interpretable results were obtained from all of 16 smear-positive and 1 of 4 smear-negative, culture-positive samples. Two of two samples containing M. tuberculosis with rpsL codon 43 mutations were correctly identified.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Rapid, simple, and culture-independent detection of rpsL codon 43 mutations that are highly predictive of streptomycin resistance in Mycobacterium tuberculosis.

Mieskes

Rüsch-Gerdes²,

Truffot-Pernot³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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“…From these studies, it became clear that there are geographic variations in the mutations that are associated with drug resistance in Mycobacterium tuberculosis (6). Especially in South America, there is a paucity of information on the distribution and types of gene mutations associated with drug resistance (7).…”

Section: N June 2008 the World Health Organization (Who) Endorsedmentioning

confidence: 99%

Predictive Value of Molecular Drug Resistance Testing of Mycobacterium tuberculosis Isolates in Valle del Cauca, Colombia

et al. 2013

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Previous evaluations of the molecular GenoType tests have promoted their use to detect resistance to first-and second-line antituberculosis drugs in different geographical regions. However, there are known geographic variations in the mutations associated with drug resistance in Mycobacterium tuberculosis, and especially in South America, there is a paucity of information regarding the frequencies and types of mutations associated with resistance to first-and second-line antituberculosis drugs. We therefore evaluated the performance of the GenoType kits in this region by testing 228 M. tuberculosis isolates in Colombia, including 134 resistant and 94 pansusceptible strains. Overall, the sensitivity and specificity of the GenoType MTBDRplus test ranged from 92 to 96% and 97 to 100%, respectively; the agreement index was optimal (Cohen's kappa, >0.8). The sensitivity of the GenoType MTBDRsl test ranged from 84 to 100% and the specificity from 88 to 100%. The most common mutations were katG S315T1, rpoB S531L, embB M306V, gyrA D94G, and rrs A1401G. Our results reflect the utility of the GenoType tests in Colombia; however, as some discordance still exists between the conventional and molecular approaches in resistance testing, we adhere to the recommendation that the GenoType tests serve as early guides for therapy, followed by phenotypic drug susceptibility testing for all cases.

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“…To be cost-effective in resource-poor countries, where most MDR-TB patients reside, it is crucial that molecular genetic tests fulfill the criteria of accuracy, speed, and simplicity. Evaluation of the frequency distribution of various mutations in clinical isolates originating from different geographical regions is essential for the selection of a limited number of target mutations to enable the detection of the majority of drug resistance (3,16).…”

Section: Correct and Rapid Diagnosis Is Essential In The Management Omentioning

confidence: 99%

Analysis for a Limited Number of Gene Codons Can Predict Drug Resistance of Mycobacterium tuberculosis in a High-Incidence Community

Rie

Warren²,

Mshanga³

et al. 2001

J Clin Microbiol

148

123

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Correct and rapid diagnosis is essential in the management of multidrug-resistant tuberculosis (MDR-TB).In this population-based study of 61 patients with drug-resistant tuberculosis, we evaluated the frequency of mutations and compared the performance of genotypic (mutation analysis by dot blot hybridization) and phenotypic (indirect proportion method) drug resistance tests. Three selected codons (rpoB531, rpoB526, and katG315) allowed identification of 90% of MDR-TB cases. Ninety percent of rifampin, streptomycin, and ethambutol resistance and 75% of isoniazid resistance were detected by screening for six codons: rpoB531, rpoB526, rrs-513, rpsL43, embB306, and katG315. The performance (reproducibility, sensitivity, and specificity) of the genotypic method was superior to that of the routine phenotypic method, with the exception of sensitivity for isoniazid resistance. A commercialized molecular genetic test for a limited number of target loci might be a good alternative for a drug resistance screening test in the context of an MDR "DOTS-plus" strategy.

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Geographic variation of the predictive values of genomic mutations associated with streptomycin resistance inMycobacterium tuberculosis

Cited by 31 publications

References 13 publications

Rapid, simple, and culture-independent detection of rpsL codon 43 mutations that are highly predictive of streptomycin resistance in Mycobacterium tuberculosis.

Rapid, simple, and culture-independent detection of rpsL codon 43 mutations that are highly predictive of streptomycin resistance in Mycobacterium tuberculosis.

Predictive Value of Molecular Drug Resistance Testing of Mycobacterium tuberculosis Isolates in Valle del Cauca, Colombia

Analysis for a Limited Number of Gene Codons Can Predict Drug Resistance of Mycobacterium tuberculosis in a High-Incidence Community

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