Studies of factors influencing spatial variation in flower size offer insights into floral evolution. We investigated altitudinal variations in five flower dimensions of two native Japanese Impatiens species (I. textori and I. noli-tangere) and their interactions with their faunal visitors. These two species have similar floral traits, including flower shape, flowering time, and pollinator species; both species are pollinated mainly by Bombus diversus. In I. textori, all measured flower dimensions were negatively correlated with altitude. In contrast, in I. noli-tangere, no measured flower dimensions correlated with altitude. Thus, the altitudinal pattern of flower size variation differed between these congeneric co-habiting herbaceous plant species. The different patterns suggest that the factors (e.g. altitudinal variations of abiotic factors) that cause variation of flower size differ between these two Impatiens species even though focal species have similar floral traits (e.g. flower shape, flowering time, and pollinator species).ARTICLE HISTORY